乌灵菌粉早期应用对创伤后应激障碍模型大鼠行为及海马组织中IL-1β、IL-6水平的影响-中国药房

乌灵菌粉早期应用对创伤后应激障碍模型大鼠行为及海马组织中IL-1β、IL-6水平的影响

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篇名：	乌灵菌粉早期应用对创伤后应激障碍模型大鼠行为及海马组织中IL-1β、IL-6水平的影响
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摘要：	目的：探讨乌灵菌粉早期应用对创伤后应激障碍（PTSD）模型大鼠行为及海马组织中白细胞介素1β（IL-1β）、IL-6水平的影响。方法：50只SD大鼠随机分为对照组（生理盐水）、模型组（生理盐水）、阳性对照组（帕罗西汀，10 mg/kg）及乌灵菌粉低、高剂量组（0.4、0.8 g/kg）。除对照组外，其他各组大鼠经增强型单次延长应激复制PTSD模型，造模后立即ig相应药物，每天1次，连续7 d。给药完成后第7天进行旷场实验（记录大鼠10 min内进入中央区次数、中央区探索时间百分比）、第8天进行高架十字迷宫实验（记录大鼠5 min内进入开臂次数百分比、开臂停留时间百分比）以观察大鼠的行为；随后处死大鼠，用酶联免疫吸附法（ELISA）测定大鼠海马组织中IL-1β、IL-6的含量。结果：与正常组比较，模型组大鼠行为学指标均显著降低，海马组织中IL-1β和IL-6的含量均显著升高（P＜0.05或P＜0.01）；与模型组比较，阳性对照组、乌灵菌粉高剂量组大鼠行为学指标均显著升高，海马组织中IL-1β和IL-6的含量均显著降低（P＜0.05），但乌灵菌粉低剂量组各指标则无显著变化（P＞0.05）。结论：高剂量乌灵菌粉可改善PTSD大鼠焦虑样行为，且这种作用可能与其降低海马组织中IL-1β、IL-6的水平有关。
ABSTRACT：	OBJECTIVE： To discuss the effect of early usage with Wulingjun powder on the behavior of rats with post-traumatic stress disorder （PTSD） and the level of interleukin-1β （IL-1β） and IL-6 in their hippocampi. METHODS： 50 SD rats were randomized into control group （normal saline）， model group （normal saline）， positive control group （paroxetine， 10 mg/kg） and the groups of low and high-dose （0.4， 0.8 g/kg） Wulingjun powder. The rats in all groups except for control group， were exposed to single prolonged stress for the establishment of PTSD models. Corresponding drugs were given ig immediately after such establishment， qd， for 7 consecutive days. An open field test （the times of the rats’ entering the central area and the percentage of the time the rats spent in exploring the central area within 10 min were recorded） was conducted 7 days after giving drugs； and an elevated plus-maze test （the times of the rats’ in opening arms and the percentage of the time the rats spent in opening arms within 5 min were recorded） was carried out 8 days thereafter， to observe the behavior of the rats. Then the rats were sacrificed to determine the level of IL-1β and IL-6 in their hippocampi by ELISA. RESULTS： Compared to the normal group， the behavioral index of the rats in model group was significantly lower， and the level of IL-1β and IL-6 in their hippocampi was significantly higher （P＜0.05 or P＜0.01）. Compared to model group， the behavioral index of the rats in positive control group and the group of high-dose Wulingjun powder was significantly higher， and the level of IL-1β and IL-6 in their hippocampi was significantly lower （P＜0.05）， but no statistically significant difference was shown in all indexes in the group of low-dose Wulingjun powder （P＞0.05）. CONCLUSIONS： High-dose Wulingjun power can improve the anxiety-like behavior of the rats with PTSD， which may be related to the reduction in the level of IL-1β and IL-6 in the hippocampus.
期刊：	2016年第27卷第25期
作者：	李凤蕾，杜菲，吴迪，彭正午
AUTHORS：	LI Fenglei，DU Fei，WU Di，PENG Zhengwu
关键字：	乌灵菌粉；大鼠；创伤后应激障碍；行为；白细胞介素1β；白细胞介素6
KEYWORDS：	Wulingjun powder； Rats； Post-traumatic stress disorder； Behavior； IL-1β； IL-6
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