星点设计-响应面法优化五味子多糖的酶提工艺
x
请在关注微信后,向客服人员索取文件
篇名: | 星点设计-响应面法优化五味子多糖的酶提工艺 |
TITLE: | |
摘要: | 目的:优化五味子多糖的酶提工艺。方法:采用星点设计-响应面法,以溶液pH、酶用量、提取温度为响应因子,以五味子多糖的提取率为响应值,在单因素试验的基础上,采用3因素5水平星点设计进行试验;同时进行验证试验。结果:优化的最佳提取工艺条件为酶提溶液pH 5.7、酶用量1.3%、提取温度53 ℃;验证试验中五味子多糖的提取率为14.30%(RSD=1.84%,n=6)。结论:优化的提取工艺简便、合理、稳定,可用于五味子多糖的提取。 |
ABSTRACT: | OBJECTIVE: To optimize enzymatic extraction technology of polysaccharide from Schisandra chinensis. METHODS: Using pH value of enzymatic extraction solution, the amount of enzyme, extraction temperature as response factor, S. chinensis polysaccharide as response value, on the basis of single-factor experiments, 3-factor, 5-level central composite experimental design was adopted for the experiment. Validation test was also conducted. RESULTS: The optimal extraction technology was as pH value of 5.7, enzyme dosage of 1.3%, extraction temperature of 53 ℃. In validation test, the extraction rate of S. chinensis polysaccharide was 14.30%(RSD=1.84%, n=6). CONCLUSIONS: The optimized extraction technology is simple, reasonable and stable, and can be used for the extraction of polysaccharide from S. chinensis. |
期刊: | 2016年第27卷第22期 |
作者: | 张楠楠,朱志军,蒋亚超,邓浩,高松,李莹丽 |
AUTHORS: | ZHANG Nannan,ZHU Zhijun,JIANG Yachao,DENG Hao,GAO Song,LI Yingli |
关键字: | 五味子多糖;酶法提取;星点设计-响应面法 |
KEYWORDS: | Schisandra chinensis polysaccharide; Enzymatic extraction technology; Central composite-response surface methodology |
阅读数: | 300 次 |
本月下载数: | 2 次 |
* 注:未经本站明确许可,任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作!