HPLC法测定脑塞通蜜丸中毛蕊花糖苷的含量
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篇名: HPLC法测定脑塞通蜜丸中毛蕊花糖苷的含量
TITLE:
摘要: 目的:建立脑塞通蜜丸中毛蕊花糖苷含量测定的方法。方法:采用高效液相色谱法。色谱柱为Zorbax ODX,流动相为乙腈-0.1%磷酸溶液(16 ∶ 84,V/V),流速为1.0 ml/min,检测波长为334 nm,柱温为30 ℃,进样量为10 μl。结果:毛蕊花糖苷检测进样量线性范围为0.200~1.000 μg(r=0.999 8);精密度、稳定性、重复性试验的RSD<1%;加样回收率为95.16%~99.78%,RSD=1.80(n=6)。结论:该方法操作简便、结果准确,适用于脑塞通蜜丸中毛蕊花糖苷的含量测定。
ABSTRACT: OBJECTIVE: To establish a method for the content determination of verbascoside in Naosaitong honeyed pill. METHODS: HPLC was performed on the column of Zorbax ODX with mobile phase of acetonitrile -0.1% phosphoric acid(16 ∶ 84, V/V) at a flow rate of 1.0 ml/min, the detection wavelength was 334 nm, column temperature was 30 ℃, and the injection volume was 10 μl. RESULTS: The linear range of verbascoside was 0.200-1.000 μg(r=0.999 8); RSDs of precision, stability and reproducibility tests were lower than 1%; recovery was 95.16%-99.78%(RSD=1.80,n=6). CONCLUSIONS: The method is simple and accurate, and suitable for the content determination of verbascoside in Naosaitong honeyed pill.
期刊: 2016年第27卷第21期
作者: 边帅,于士婷,白雪媛,赵大庆,赵雨,王丽丽
AUTHORS: BIAN Shuai,YU Shiting,BAI Xueyuan,ZHAO Daqing,ZHAO Yu,WANG Lili
关键字: 脑塞通蜜丸;毛蕊花糖苷;高效液相色谱法
KEYWORDS: Naosaitong honeyed pill; Verbascoside; HPLC
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