马鞭草总苷对小鼠慢性非细菌性前列腺炎的影响及其抗炎、镇痛作用研究-中国药房

马鞭草总苷对小鼠慢性非细菌性前列腺炎的影响及其抗炎、镇痛作用研究

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篇名：	马鞭草总苷对小鼠慢性非细菌性前列腺炎的影响及其抗炎、镇痛作用研究
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摘要：	目的：研究马鞭草总苷对小鼠慢性非细菌性前列腺炎（CNP）的影响及其抗炎、镇痛作用。方法：取60只小鼠随机分为正常组（生理盐水）、模型组（生理盐水）、阳性组（前列康片1.5 g/kg）和马鞭草总苷高、中、低剂量组（0.2、0.1、0.05 g/kg），每组10只，除正常组外，其余各组小鼠复制CNP模型；从造模第8天开始，各组小鼠ig相应药物，每天1次，连续21 d；观察小鼠前列腺组织白细胞数、卵磷脂小体密度和病理形态变化。分别取50只小鼠随机分为模型组（生理盐水）、阳性组（阿司匹林片0.3 g/kg或醋酸地塞米松片0.01 g/kg）和马鞭草总苷高、中、低剂量组（0.2、0.1、0.05 g/kg），ig给药，每天1次，连续7 d；进行二甲苯致小鼠耳廓肿胀实验（阳性药物为阿司匹林片）和小鼠棉球肉芽肿实验（阳性药物为醋酸地塞米松片），测定小鼠耳廓肿胀度、肉芽肿净质量；进行小鼠醋酸扭体实验和小鼠热板舔足实验（阳性药物均为阿司匹林片），测定小鼠扭体潜伏期、20 min内扭体次数、痛阈值。结果：与模型组比较，各给药组小鼠前列腺组织白细胞数减少、卵磷脂小体密度升高，阳性组和马鞭草总苷高、中剂量组小鼠病理形态明显改善，多集中于0、Ⅰ级；阳性组和马鞭草总苷高、中剂量组小鼠耳廓肿胀度降低、肉芽肿净质量减少；各给药组小鼠20 min内扭体次数减少，阳性组和马鞭草总苷高剂量组小鼠扭体反应潜伏期延长、痛阈值提高，以上差异均有统计学意义（P＜0.05或P＜0.01）。结论：马鞭草总苷对小鼠CNP具有改善作用，并表现出良好的抗炎、镇痛作用。
ABSTRACT：	OBJECTIVE： To study the effects of total glucosides of Verbena officinalis on chronic nonbacterial prostatitis （CNP） in mice and its anti-inflammatory and analgesic effects. METHODS： 60 mice were randomized into normal group （normal saline）， model group （normal saline）， positive group （Qianliekang tablet 1.5 g/kg） and total glucosides of V. officinalis high-dose， medium-dose and low-dose groups （0.2， 0.1， 0.05 g/kg）， with 10 mice in each group. CNP model was induced in those groups except for normal group； mice were given relevant medicine once a day for consecutive 21 days since 8th day of modeling. The white blood cell count， lecithin corpuscle density and pathological changes of prostate samples were observed. 50 mice were randomly divided into model group （normal saline）， positive group （Aspirin tablet 0.3 g/kg or Dexamethasone acetate tablet 0.01 g/kg） and total glucosides of V. officinalis high-dose， medium-dose and low-dose groups （0.2， 0.1， 0.05 g/kg）； they were given relevant medicine intragastrically once a day for consecutive 7 days. The xylene-induced ear swelling test （Aspirin tablet as positive drug） and cotton-pellet granuloma test （Dexamethasone acetate tablet as positive drug） were conducted to determine the the degree of ear swelling and the net weight of granuloma. The acetic acid writhing test and hot plate test （Aspirin tablet as positive drug） were carried out to determine the latency period of writhing， the times of writhing within 20 min and pain threshold. RESULTS： Compared with model group， white blood cells count of prostate tissue were lowered while the density of lecithin corpuscles were increased significantly in medicine groups； the pathology of prostate tissue were improved significantly in positive group and total glucosides of V. officinalis high-dose and medium-dose groups， and mostly were 0 and Ⅰ grade. The degree of ear swelling and the net weight of granuloma were markedly reduced in positive group and total glucosides of V. officinalis high-dose and medium-dose groups； the times of writhing within 20 min decreased in medicine groups， and the latency period of writhing prolonged and pain threshold increased in positive group and total glucosides of V. officinalis high-dose group. All of the results showed statistical difference（P＜0.05 or P＜0.01）. CONCLUSIONS： Total glucosides of V. officinalis can improve CNP in mice， and shows good anti-inflammatory and analgesic effects.
期刊：	2016年第27卷第19期
作者：	王琳琳，王灿，苗明三
AUTHORS：	WANG Linlin，WANG Can，MIAO Mingsan
关键字：	马鞭草总苷；慢性非细菌性前列腺炎；抗炎；镇痛；小鼠
KEYWORDS：	Total glucosides of Verbena officinalis； Chronic nonbacterial prostatitis； Anti-inflammatory； Analgesic； Mice
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