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甲基阿魏酸对乙型肝炎病毒转基因小鼠的抗病毒作用研究
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| 篇名： | 甲基阿魏酸对乙型肝炎病毒转基因小鼠的抗病毒作用研究 |
| TITLE： | |
| 摘要： | 目的：研究甲基阿魏酸（MFA）对乙型肝炎病毒（HBV）转基因小鼠的抗病毒作用。方法：将SPF级HBV转基因BALB/c小鼠随机分为模型对照组、阳性药物组（拉米夫定100 mg/kg）、MFA组（100 mg/kg），每组8只；另取同种同龄非转基因BALB/c小鼠为正常对照组。除模型对照组和正常对照组小鼠ig等剂量蒸馏水外其余各组ig相应药物，每日1次。观察各组小鼠体质量，分别于给药前和给药后7、14、21 d，以及停药3 d采集全血约0.25 ml；检测血清中丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）活性，实时荧光定量-聚合酶链式反应检测血清中HBV-DNA的拷贝数，酶联免疫吸附法检测血清中HBV外膜大蛋白（HBV-LP）的相对含量。结果：各组小鼠体质量及ALT、AST活性比较差异无统计学意义（P＞0.05）。与模型对照组比较，MFA组小鼠给药后各时间点及停药后血清中HBV-DNA和HBV-LP水平均降低（P＜0.05或P＜0.01）；拉米夫定组小鼠给药后各时间点血清中HBV-DNA的拷贝数降低（P＜0.05或P＜0.01），而HBV-LP的相对含量仅在药后21 d时降低（P＜0.05），停药则立即回升。结论：MFA可有效抑制HBV转基因小鼠血清中HBV-DNA和HBV-LP水平，未见明显反跳现象；对小鼠血清转氨酶和体质量未见明显影响。 |
| ABSTRACT： | OBJECTIVE： To study the antivirus effects of methyl ferulic acid （MFA） in HBV transgenic mice. METHODS： Specific pathogen frce（SPF）level HBV transgenic BALB/c mice were randomly divided into model control group， positive drug group （lamivudine 100 mg/kg）and MFA group （100 mg/kg） with 8 mice in each group； non-transgenic BALB/c mice with the same age and congener were included in normal control group. Mice were given relevant medicine intragastrically once a day except model control group and normal control group were given constant volume of solution intragastrically. Blood 0.25 ml were collected respectively before treatment and at the 7th， 14th， 21st day during treatment， and at 3rd day after drug withdrawal. Body weight， serum levels of ALT and AST were observed in each group. The serum copies of HBV-DNA were detected by RT fluorescent quantitative-PCR， and relative serum content of HBV large envelope protein （HBV-LP） was detected by ELISA. RESULTS： There was no statistical significance in body weight， the activity of ALT and AST among those groups （P＞0.05）. Compared with model control group， the level of HBV-DNA and HBV-LP in serum of mice decreased in MFA group at each time point after medication and after drug withdrawal （P＜0.05 or P＜0.01）； serum copies of HBV-DNA in mice decreased in lamivudine group at different time points after medication （P＜0.05 or P＜0.01）， and relative content of HBV-LP decreased only at 21st day after medication （P＜0.05）， but increased immediately after drug withdrawal. CONCLUSIONS： MFA can effectively inhibit the level of HBV-LP and HBV-DNA in serum without obvious bounce phenomenon in HBV transgenic mice； it shows no obvious effects on serum transaminase and body weight. |
| 期刊： | 2016年第27卷第13期 |
| 作者： | 杨成芳，李丽，李勇文，容明智，庞勇军，方舒萍，韦冰华 |
| AUTHORS： | YANG Chengfang，LI Li，LI Yongwen，RONG Mingzhi，PANG Yongjun，FANG Shuping，WEI Binghua |
| 关键字： | 甲基阿魏酸；乙型肝炎病毒；转基因小鼠；乙型肝炎病毒外膜大蛋白；脱氧核糖核酸 |
| KEYWORDS： | Methyl ferulic acid； HBV； Transgenic mice； HBV large envelope protein； DNA |
| 阅读数： | 179 次 |
| 本月下载数： | 3 次 |
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