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妥洛特罗体内外非特异性抗炎作用研究
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| 篇名： | 妥洛特罗体内外非特异性抗炎作用研究 |
| TITLE： | |
| 摘要： | 目的：研究妥洛特罗体内外非特异性抗炎作用。方法：设立空白对照，采用MTT比色法观察妥洛特罗对小鼠腹腔原代巨噬细胞增殖的影响，Griess法观察妥洛特罗对脂多糖刺激原代巨噬细胞NO释放的影响，酶联免疫吸附法观察妥洛特罗对脂多糖刺激原代巨噬细胞中炎症因子TNF-α、IL-1β、IL-6、IL-17A生成的影响；同时，设立模型组，观察妥洛特罗贴片皮肤局部给药对巴豆油诱导的耳炎模型小鼠耳肿胀度、耳肿胀抑制率的影响和对1％2，4-二硝基氟苯（DNFB）诱导的迟发型过敏（DTH）模型小鼠耳肿胀度、耳肿胀抑制率、体质量和胸腺、脾脏的脏器质量指数的影响。结果：与空白对照比较，妥洛特罗（1×10－4～1×10－3 μmol/L）对原代巨噬细胞的增殖（半数抑制浓度为13.67×10－4 mol/L）、IL-17A的生成无明显影响，可明显抑制NO的释放（半数抑制浓度为5.45×10－4 mol/L）和炎症因子TNF-α、IL-1β、IL-6的生成（P＜0.05或P＜0.01）。与模型组比较，妥洛特罗贴片皮肤局部给药后耳炎模型和DTH模型小鼠的耳肿胀度明显减小（P＜0.05或P＜0.01）、耳肿胀抑制率增加，但体质量和胸腺、脾脏的脏器质量指数无明显变化。结论：妥洛特罗在体内外试验中均表现出一定的抗炎作用。 |
| ABSTRACT： | OBJECTIVE： To study non-specific anti-inflammation effects of tulobuterol in vitro and in vivo. METHODS： By setting up blank control， MTT colorimetry was used to observe the effects of tulobuterol on the proliferation of primary mice peritoneal macrophages； the effects of tulobuterol on NO release in primary macrophages stimulated with lipopolysaccharide were detected by Griess； the effects of tulobuterol on the generation of inflammatory factors TNF-α， IL-1β， IL-6 and IL-17A in primary macrophages stimulated with lipopolysaccharide were determined by ELISA. Meanwhile， by setting up model group， the effects of Tulobuterol patch for local use on ear swelling degree and inhibitory rate of ear swelling in soybean oil induced ear inflammation model mice were observed as well as its effects on ear swelling degree， inhibitory rate of ear swelling and body weight， organ mass index of thymus gland and spleen in 1％ 2,4-dinitrofluorobenzene （DNFB） induced delayedtype hypersensitivity （DTH） model mice. RESULTS： Compared with blank control， tulobuterol （1×10－4-1×10－3 μmol/L） had not significant inhibitory effect on the proliferation of primal macrophages （IC50＝13.67×10－4 mol/L） and generation of IL-17A， but significantly inhibited the generation of NO （IC50＝5.45×10－4 mol/L） and inflammatory factors TNF-α， IL-1β and IL-6 （P＜0.05 or P＜0.01）. Compared with model group， ear swelling degree of ear inflammation model mice and DTH model mice relieved significantly（P＜0.05 or P＜0.01）， while inhibitory rate of ear swelling increased and body weight， mass index of thymus gland and spleen had no significant change after receiving Tulobuterol patch for local use. CONCLUSIONS： Tulobuterol shows a certain anti-inflammatory effect in vitro and in vivo. |
| 期刊： | 2016年第27卷第13期 |
| 作者： | 傅丽霞，张宇佳，庄媛媛，关晶，王霞，高蕊，白金叶，侯琦，郑稳生，林明宝，张建民 |
| AUTHORS： | FU Lixia，ZHANG Yujia，ZHUANG Yuanyuan，GUAN Jing，WANG Xia，GAO Rui，BAI Jinye，HOU Qi，ZHENG Wensheng，LIN Mingbao，ZHANG Jianmin |
| 关键字： | 妥洛特罗；贴片；体内；体外；巨噬细胞；小鼠；抗炎 |
| KEYWORDS： | Tulobuterol； Patch； in vivo； in vitro； Macrophages； Mice； Anti-inflammation |
| 阅读数： | 554 次 |
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