米非司酮对子宫内膜异位症模型大鼠炎症因子表达的影响
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篇名: 米非司酮对子宫内膜异位症模型大鼠炎症因子表达的影响
TITLE:
摘要: 目的:研究米非司酮对子宫内膜异位症(Ems)模型大鼠炎症因子表达的影响。方法:取SD大鼠,随机分为正常(生理盐水)组、模型(生理盐水)组和米非司酮低、中、高剂量[0.65、1.30、2.60 mg/(kg·d)]组,每组10只。除正常组外,其余各组大鼠复制Ems模型,各组大鼠ig相应剂量的药物,连续4周。比较各组大鼠治疗前后异位病灶体积,酶联免疫吸附法检测各组大鼠血清中肿瘤坏死因子α(TNF-α)、白细胞介素(IL)-6、IL-8含量,Western blot法分析各组大鼠异位病灶组织中环氧化酶2(COX-2)、前列腺素E2(PGE2)蛋白表达及核因子κB(NF-κB)p65磷酸化水平,实时定量-聚合酶链反应法检测各组大鼠异位病灶组织中NF-κB p65 mRNA表达水平。结果:与正常组比较,模型组大鼠异位病灶体积和血清TNF-α、IL-6、IL-8含量增加,病灶组织中COX-2、PGE2蛋白和NF-κB p65磷酸化水平及mRNA表达增强(P<0.01);与模型组比较,米非司酮各剂量组大鼠异位病灶体积减小,血清TNF-α、IL-6、IL-8含量降低,病灶组织中COX-2、PGE2和NF-κB p65磷酸化水平及mRNA表达减弱(P<0.01),且呈剂量依赖性。结论:米非司酮能减小Ems异位病灶体积,可能与抑制NF-κB信号通路和降低炎症因子表达有关。
ABSTRACT: OBJECTIVE: To study the effects of mifepristone on the expression of inflammatory factors in endometriosis (Ems) model rats. METHODS: SD rats were randomly divided into normal group (normal saline), model group (normal saline), mifepristone low-dose, medium-dose and high-dose groups [0.65, 1.30, 2.60 mg/(kg·d)], with 10 rats in each group. Except for normal group, Ems model was induced in other groups, and they were given relevant medicine intragastrically for consecutive 4 weeks. The volume of the ectopic focus was compared before and after treatment. The serum contents of TNF-α, IL-6 and IL-8 were detected by ELISA method. The phosphorylation of NF-κB p65 and the expression of COX-2 and PGE2 were detected by Western blot. The expression of NF-κB p65 mRNA in ectopic focus was detected by RT-PCR. RESULTS: Compared with normal group, the volume of the ectopic focus, the serum contents of TNF-α, IL-6 and IL-8, the expression of COX-2 and PGE2 protein, the phosphorylation of NF-κB p65 and the expression of NF-κB p65 mRNA in ectopic focus were increased in model group (P<0.01). Compared with model group, the volume of the ectopic focus, the serum contents of TNF-α, IL-6 and IL-8, the expression of COX-2 and PGE2 protein, the phosphorylation of NF-κB p65 and the expression of NF-κB p65 mRNA in ectopic focus were decreased in mifepristone groups (P<0.01), in dose-dependent manner. CONCLUSIONS: Mifepristone can reduce the volume of the Ems ectopic focus, via blocking NF-κB signaling pathway and reducing the expression of inflammatory factors.
期刊: 2016年第27卷第10期
作者: 马永萍,张青雯
AUTHORS: MA Yongping,ZHANG Qingwen
关键字: 米非司酮;子宫内膜异位症;大鼠;炎症因子
KEYWORDS: Mifepristone; Endometriosis; Rats; Inflammatory factor
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