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千年健挥发油对佐剂性关节炎模型大鼠的药效作用及其机制 研究
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| 篇名： | 千年健挥发油对佐剂性关节炎模型大鼠的药效作用及其机制 研究 |
| TITLE： | |
| 摘要： | 目的：研究千年健挥发油对佐剂性关节炎（AA）大鼠的药效作用及其机制。方法：取60只大鼠随机分为正常对照（0.5％聚山梨酯80）组、模型对照（0.5％聚山梨酯80）组、阳性对照（雷公藤多苷片，10 mg/kg）组和千年健挥发油高、中、低剂量（0.08、0.04、0.02 ml/kg）组，除正常对照组外，其余各组大鼠均于右后足跖部ih完全弗氏佐剂复制大鼠AA模型，造模当日开始连续ig给药25 d，每天1次。检测大鼠足关节肿胀度、免疫器官（胸腺、脾脏）脏器指数、组织病理学变化以及血清中白细胞介素1β（IL-1β）、肿瘤坏死因子α（TNF-α）的含量。结果：与正常对照组比较，模型对照组大鼠原发性和继发性关节肿胀度、胸腺指数升高，脾脏指数降低，血清中IL-1β、TNF-α含量增加（P＜0.05或P＜0.01）；足关节可见明显组织肿胀，大量中性粒细胞、淋巴细胞及巨噬细胞浸润关节周围组织，滑膜细胞增生，呈明显关节炎性病变。与模型对照组比较，千年健挥发油各剂量组大鼠原发性和继发性关节肿胀度降低、血清中IL-1β含量减少，千年健挥发油中、低剂量组大鼠胸腺指数升高，千年健挥发油高剂量组大鼠血清中TNF-α含量减少（P＜0.05或P＜0.01）；各给药组大鼠关节周围组织炎性细胞浸润减轻，关节滑膜细胞增生不明显，形态得到改善。结论：千年健挥发油对AA大鼠有一定的药效作用，其机制可能与减少血清中IL-1β、TNF-α含量有关。 |
| ABSTRACT： | OBJECTIVE： To study the pharmacodynamic effects of volatile oil from Homalomena occulta on adjuvant-induced arthritis （AA） model rats and its mechanism. METHODS： 60 rats were randomly divided into normal control group （0.5％ polysorbate 80）， model control group （0.5％ polysorbate 80）， positive control group （Tripterygium glycosides tablets， 10 mg/kg）， high-dose， middle-dose and low-dose （0.08， 0.04 and 0.02 ml/kg） groups of volatile oil from H. occulta. Except for normal control group， other groups were given complete Freund’s adjuvant subcutaneously via right rear foot plantar to induce AA model， and given relevant medicine intragastrically for 25 days， once a day， since modeling. The articular swelling degree， immune organ （thymus gland and spleen） index， pathological change， the contents of IL-1β and TNF-α in serum were detected. RESULTS： Compared with normal control group， the primary and secondary articular swelling degree and thymus gland index of rats and the serum contents of IL-1β and TNF-α increased in model control group， while spleen index decreased （P＜0.05 or P＜0.01）； obvious tissue swelling， large amount of neutrophile granulocyte， leukomonocyte and macrophage infiltrating joint surrounding tissue， the proliferation of synovial cells and obvious osteoarthritic lesion were observed in podarthrum. Compared with model control group， the primary and secondary articular swelling degree and the serum content of IL-1β decreased in the volatile oil from H. occulta groups； thymus gland index increased in middle-dose and low-dose groups of the volatile oil from H. occulta； the content of TNF-α decreased in high-dose group of the volatile oil from H. occulta （P＜0.05 or P＜0.01）. The inflammatory cell infiltration of joint surrounding tissue relieved in treatment groups， synovial cells proliferation was not obvious and synovial cells morphology was improved. CONCLUSIONS： The volatile oil from H. occulta has the pharmacodynamic effects on AA in rat， and its mechanism might be related to the serum content reduction of IL-1β and TNF-α. |
| 期刊： | 2016年第27卷第10期 |
| 作者： | 胡远，李晋奇，张舒涵，何丹 |
| AUTHORS： | HU Yuan，LI Jinqi，ZHANG Shuhan，HE Dan |
| 关键字： | 千年健；挥发油；佐剂性关节炎；药效作用；白细胞介素1β；肿瘤坏死因子α；大鼠 |
| KEYWORDS： | Homalomena occulta； Volatile oil； Adjuvant-induced arthritis； Pharmacodynamic effect； IL-1β； TNF-α； Rat |
| 阅读数： | 198 次 |
| 本月下载数： | 2 次 |
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