内毒素吸附剂SPV对创伤失血性休克模型大鼠肠黏膜损伤的保护作用-中国药房

内毒素吸附剂SPV对创伤失血性休克模型大鼠肠黏膜损伤的保护作用

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篇名：	内毒素吸附剂SPV对创伤失血性休克模型大鼠肠黏膜损伤的保护作用
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摘要：	目的：研究内毒素吸附剂SPV对创伤失血性休克模型大鼠肠黏膜损伤的保护作用。方法：将0.5 mg多黏菌素B、5 mg维生素B6、0.3 g蒙脱石散溶于生理盐水中制成5 ml溶液，即SPV低剂量；中、高剂量药物成分为低剂量的2、3倍。取大鼠随机分为正常对照组、模型组和SPV低、中、高剂量组，每组10只。除正常对照组外，其余各组大鼠通过股动脉插管放血术复制创伤失血性休克模型，建模前30 min SPV各剂量组大鼠ig相应剂量的SPV。复苏后1、4、8、16 h，检测各组大鼠血清中一氧化氮（NO）、二胺氧化酶（DAO）、内毒素及肿瘤坏死因子α（TNF-α）水平，观察正常对照组、模型组和SPV高剂量组大鼠肠黏膜细胞特征和损伤指数。结果：与正常对照组比较，模型组大鼠肠黏膜细胞出现坏死的病理现象，血清中NO、DAO、内毒素和TNF-α水平均升高（P＜0.05）。与模型组比较，SPV中、高剂量组大鼠复苏后1、4、8、16 h肠黏膜细胞损伤程度明显减轻，血清中NO、DAO、内毒素和TNF-α水平均降低（P＜0.05）；其余各组比较差异无统计学意义（P＞0.05）。结论：内毒素吸附剂SPV能够降低创伤失血性休克模型大鼠血清中NO、DAO、内毒素及TNF-α水平，对创伤失血性休克引起的肠黏膜损伤有保护作用。
ABSTRACT：	OBJECTIVE： To study the protective effects of endotoxin adsorbent SPV on intestinal mucosa injury in rats with traumatic and hemorrhagic shock. METHODS： 0.5 mg polymyxin B， 5 mg vitamine B6 and 0.3 g Montmorillonite powder were dissolved in normal saline to prepare 5 ml solution， i.e. low-dose of SPV. Medium-dose and high-dose drug were 2 and 3 folds of low- dose. Rats were randomly divided into normal control group， model group and SPV low-dose， medium-dose and high-dose groups with 10 rats in each group. Except for normal control group， other groups were given by femoral artery bloodletting to induce traumatic and hemorrhagic shock model. 30 min before modeling， rats of SPV groups were given relevant dose of SPV intragastrically. 1， 4， 8 and 16 h after resuscitation， the serum levels of NO， DAO， endotoxin and TNF-α were detected in all rats. The intestinal mucosa cells characteristics and intestinal mucosa lesion index were observed in normal control group， model group and SPV high-dose group. RESULTS： Compared with normal control group， intestinal mucosa cells necrosis of model group was found， and the serum levels of NO， DAO， endotoxin and TNF-α increased （P＜0.05）. Compared with model group， intestinal mucosa cells injury relieved significantly in SPV high-dose and medium-dose groups 1， 4， 8 and 16 h after resuscitation， and the serum levels of NO， DAO， endotoxin and TNF-α decreased （P＜0.05）； there was no significant difference of the other groups（P＞0.05）. CONCLUSIONS： The endotoxin adsorbent SPV can significantly reduce the serum levels of NO， DAO， endotoxin and TNF-α in rats with traumatic and hemorrhagic shock， it has protective effect on intestinal mucosal injury induced by traumatic and hemorrhagic shock.
期刊：	2016年第27卷第10期
作者：	刘海，赵剑秋，彭晓静，郭平选，王太，张建新，张辉，席文娟
AUTHORS：	LIU Hai， ZHAO Jianqiu， PENG Xiaojing， GUO Pingxuan，WANG Tai， ZHANG Jianxin， ZHANG Hui， XI Wenjuan
关键字：	内毒素吸附剂；创伤失血性休克；内毒素；一氧化氮；二胺氧化酶；肿瘤坏死因子α；大鼠
KEYWORDS：	Endotoxin adsorbent； Traumatic and hemorrhagic shock； Endotoxin； NO； DAO； TNF-α； Rats
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