蒙药肋柱花水提物对D-GlaN和CCl4致小鼠急性肝损伤的保护作用研究
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篇名: 蒙药肋柱花水提物对D-GlaN和CCl4致小鼠急性肝损伤的保护作用研究
TITLE:
摘要: 目的:研究蒙药肋柱花水提物对D-氨基半乳糖胺(D-GlaN)和四氯化碳(CCl4)致小鼠急性肝损伤的保护作用。方法:取60只小鼠随机分为正常(生理盐水)组、D-GlaN模型(生理盐水)组、阳性对照(葵花护肝片,0.56 g/kg)组和肋柱花水提物高、中、低剂量[3、1.5、0.75 g(生药)/kg]组,每组10只,ig给药,qd,连续23 d;末次给药30 min后,除正常组外,其余各组小鼠ip D-GlaN复制急性肝损伤模型。检测小鼠血清中天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、碱性磷酸酶(ALP)活性和胆碱酯酶(CHE)含量,计算肝脏指数,对肝组织病理形态进行观察并评分。另取60只小鼠,除造模方法(ig 0.1% CCl4)不同外,按相同分组、给药及指标检测方法进行实验。结果:与正常组比较,两种模型组小鼠肝细胞明显坏死或变性,病理评分升高;血清中AST、ALT、ALP活性升高, CHE含量减少;且D-GlaN模型组肝脏指数升高、CCl4模型组肝脏指数降低(P<0.05)。与D-GlaN模型组比较,肋柱花水提物各剂量组小鼠血清AST、ALT、ALP活性及肝脏指数降低,CHE含量增加(P<0.05);肝组织病理形态改善,评分降低。与CCl4模型组比较,肋柱花水提物低剂量组小鼠血清ALT活性降低、CHE含量增加,肝脏指数升高;肋柱花水提物中剂量组小鼠血清CHE  含量减少,肝脏指数升高;肋柱花水提物高剂量组小鼠血清AST、ALT、ALP活性降低,CHE含量增加(P<0.05)。肋柱花水提物各剂量组小鼠肝组织病理形态都得到一定改善,评分降低。结论:肋柱花水提物对D-GlaN或CCl4致小鼠急性肝损伤有一定保护作用。
ABSTRACT: OBJECTIVE: To study the protective effects of Mongolian medicine Lomatogonium rotatum water extract against acute liver injury in mice caused by D-GlaN and CCl4. METHODS: 60 mice were randomly divided into normal group (normal saline), D-GlaN model group (normal saline), positive control group (Kuihua hugan tablet, 0.56 g/kg) and L. rotatum water extract high-dose, medium-dose and low-dose groups [3, 1.5, 0.75 g(crude drug)/kg] 10 rats in each group. They were given relevant medicine intragastrically once a day for consecutive 23 days. 30 min after last administration, those group were given D-GlaN ip to induce acute liver injury model except normal group. The activity of AST, ALT, ALP, and acetylcholinesterase (CHE) content in serum were detected, and liver index was calculated. Liver histopathology was observed and scored. Other 60 mice were selected, and then grouped, given medicine and detected in lab index with same method with above group, except for modeling method (ig, 0.1% CCl4). RESULTS: Compared with normal group, necrosis or degeneration of liver cells were obvious in 2 model groups, pathological score, the activity of AST, ALT and ALP increased while CHE content decreased; liver index of D-GlaN model group increased while that of CCl4 model group decreased (P<0.05). Compared with D-GlaN model group, the activity of AST, ALT and ALP and liver index decreased in L. rotatum water extract groups, while CHE content increased (P<0.05); pathological morphology had been improved, and pathological score decreased. Compared with CCl4 model group, ALT activity decreased in L. rotatum water extract low-dose group, while CHE content and liver index increased; CHE content of L. rotatum water extract medium-dose group decreased, while liver index increased; the activity of AST, ALT and ALP decreased in L. rotatum water extract high-dose group, while CHE content increased (P<0.05). Pathological morphology of L. rotatum water extract groups had been improved to certain extent, and pathological score decreased. CONCLUSIONS: L. rotatum water extract could protect acute liver injury in mice induced by D-GlaN and CCl4.
期刊: 2016年第27卷第10期
作者: 包明兰,巴根那,辛颖,白梅荣,何那拉
AUTHORS: BAO Minglan,Bagenna,XIN Ying,BAI Meirong,HE Nala
关键字: 蒙药;肋柱花水提物;D-氨基半乳糖胺;四氯化碳;急性肝损伤;保护作用;小鼠
KEYWORDS: Mongolian medicine; Lomatogonium rotatum water extract; D-GlaN; CCl4; Acute liver injury; Protective effect; Mice
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