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烟叶中茄尼醇的超声提取及纯化工艺研究
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| 篇名： | 烟叶中茄尼醇的超声提取及纯化工艺研究 |
| TITLE： | |
| 摘要： | 目的：优化烟叶中茄尼醇的超声提取及纯化工艺。方法：分别以茄尼醇提取率和转移率为指标，采用单因素试验对提取工艺中的料液比、超声提取温度、超声提取时间、超声功率、提取次数，以及纯化工艺中的皂化液用量（皂化液与提取液体积比）、酸化液用量（酸化液与皂化后提取液体积比）、萃取次数等因素水平进行考察，对优化后工艺进行验证，计算茄尼醇纯度；另将本法与传统浸提法（耗时30 h）和溶剂连续循环浸提法（耗时5～6 h）比较茄尼醇的提取量。结果：优化的提取工艺为料液比1 ∶ 14、超声提取温度70 ℃、超声提取时间60 min、超声功率120 W、提取3次，纯化工艺为皂化液与提取液体积比2 ∶ 35、酸化液与皂化后提取液体积比2 ∶ 14、萃取4次；验证试验中提取率、转移率和纯度均值分别为92.45％、79.88％、55.86％（RSD分别为0.46％、0.30％、0.40％，n＝3）；3种提取方法茄尼醇的提取量分别为52.22、45.22、26.10 mg/g。结论：优化工艺操作简单、稳定，提取耗时更短，更节约能源，且提取量和纯度均较高，适合用于烟叶中茄尼醇的生产提取及纯化。 |
| ABSTRACT： | OBJECTIVE： To optimize ultrasonic extraction and purification technology of solanesol from tobacco leaf. METHODS： Using extraction rate and transport rate of solanesol as indexes， single factor test was used to investigate liquid-solid ratio， ultrasonic extraction temperature and time， ultrasonic power and extraction times， and the amount of soap alkali lye （volume ratio of soap alkali lye to extraction liquid）， acidizing fluids （volume ratio of acidizing fluids to soap alkali lye extract）， extraction times of purification technology. Optimized technology was validated， and the purity of solanesol was calculated； the amount of extracted solanesol was compared between this method and traditional extraction method （spending 30 h）， solvent continuous cyclic extraction （spending 5-6 h）. RESULTS： Optimized extraction technology was as follows as volume ratio of soap alkali lye to extraction liquid 1 ∶ 14， ultrasonic extraction temperature 70 ℃， ultrasonic extraction time 60 min， ultrasonic power 120 W， extracting for 3 times； optimized purification technology was as follows as volume ratio of soap alkali lye to extraction liquid 2 ∶ 35， volume ratio of acidizing fluids to soap alkali lye extract 2 ∶ 14， extracting for 4 times. In validation test， extraction rate， transport rate and purity were 92.45％ （RSD＝0.46％， n＝3）， 79.88％ （RSD＝0.30％， n＝3） and 55.86％ （RSD＝0.40％， n＝3）. The amount of solanesol extracted with 3 methods were 52.22， 45.22 and 26.10 mg/g. CONCLUSIONS： The optimized technology is simple and stable， costs less time and saves source with high extraction amount and purity， which is suitable for production， extraction and purification of solanesol from tobacco leaf. |
| 期刊： | 2016年第27卷第7期 |
| 作者： | 张艺，王刚，陈振斌，邸多隆 |
| AUTHORS： | ZHANG Yi，WANG Gang，CHEN Zhenbin，DI Duolong |
| 关键字： | 烟叶；茄尼醇；单因素试验；超声提取；纯化；工艺优化；提取率；转移率；纯度 |
| KEYWORDS： | Tobacco leaf； Solanesol； Single factor test； Ultrasonic extraction； Purification； Technology optimization； Extraction rate； Transport rate； Purity |
| 阅读数： | 237 次 |
| 本月下载数： | 3 次 |
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