目的：研究黄芩苷对人骨肉瘤MG63 细胞凋亡及基质金属蛋白酶（MMPs）表达的影响。方法：以0（空白对照）、5、10、20、

40、80、160、320 μg/ml 黄芩苷处理MG63 细胞24 h 后，检测细胞存活率和细胞中MMP-2、MMP-9 的表达量，计算半数抑制浓度

（IC50），观察细胞凋亡情况。结果：与空白对照比较，黄芩苷处理MG63 细胞后细胞存活率降低，凋亡细胞数量增加，细胞中

MMP-2 和MMP-9 表达量降低，并以320 μg/ml 黄芩苷处理时细胞中MMP-2 和MMP-9 表达量下降具有统计学意义（P＜0.05）；IC50

为（40.21±9.20）μg/ml，各作用效应均呈浓度依赖性。结论：黄芩苷可抑制MG63 细胞增殖，诱导其凋亡，较高质量浓度时可抑制

细胞中MMP-2、MMP-9 的表达。

OBJECTIVE：To study the effects of baicalin on human osteosarcoma MG63 cells apoptosis and the expression of

MMPs. METHODS：Treated with 0（blank control），5，10，20，40，80，160，320 μg/ml baicalin for 24 h，the survival rate，the

expression amount of MMP-2 and MMP-9 were detected，and IC50 was calculated. Cell apoptosis was observed. RESULTS：Compared

with blank control group，after treated with baicalin，survival rate of MG63 cells decreased，while apoptotic amount increased，

the expression amount of MMP-2 and MMP-9 decreased；there was statistical significance in the expression amount decrease

of MMP-2 and MMP-9 in MG63 cells after treated with 320 μg/ml baicalin（P＜0.05）；IC50 was（40.21±9.20）μg/ml，all

responses were in concentration-dependent manner. CONCLUSIONS：Baicalin can inhibit the proliferation of MG63 cells，induce

cell apoptosis，and inhibit the expression of MMP-2 and MMP-9 in cells under high concentration.