目的：阐明芹菜素在大鼠体内的生物转化形式，推测其可能的代谢途径。方法：取大鼠分为空白组和给药组（灌胃给予芹

菜素，200 mg/kg），每组6 只。给药24 h 内分别收集两组的尿液和粪便，进行相应处理后同时在正离子模式和负离子模式下采用高

效液相串联离子阱飞行时间多级质谱法进行分析检测。结果：从给药组的大鼠尿液中鉴定出9 个代谢产物，分别为芹菜素发生2，

3 位双键还原（U1、U7、U8、U9）、与葡萄糖醛酸结合（U2、U3、U4）、与硫酸酯结合（U5、U6、U7、U8、U9）、与葡萄糖结合的产物（U2）；

从给药组的大鼠粪便样品中鉴定出4 个代谢产物，分别为芹菜素发生2，3 位双键还原（代谢产物F3）、与葡萄糖醛酸结合（F2）、与

葡萄糖结合的产物（F1）。结论：芹菜素在大鼠体内主要仍以原型药物存在，推测在肠道细菌的作用下可发生2，3 位双键的还原，

在肠道与体内排泄时均可形成葡萄糖醛酸或葡萄糖结合产物，而形成硫酸酯结合产物则仅在体内排泄时发生。

OBJECTIVE：To clarify the bio-transformation form of apigenin in rats，and to speculate its possible metabolic pathway.

METHODS：Rats were divided into blank group and medication group（apigenin 200 mg/kg，i.g.）with 6 rats in each group.

Urine and feces samples were collected from 2 groups within 24 h after medication. After corresponding treatment，urine and feces

samples were analyzed and detected by HPLC-IT-TOF-MSn under cation mode and anion mode. RESULTS：9 metabolites were identified

in urine sample of rats from medication group，i.e. 2，3-double bond reduction of apigenin（U1，U7，U8，U9），bonded to

glucuronic acid（U2，U3，U4），bonded to sulphate（U5，U6，U7，U8，U9）and bonded to glucose（U2）. 4 metabolites were identified

in feces sample of rats from medication group，i.e. 2，3-double bond reduction of apigenin（F3），bonded to glucuronic acid

（F2）and bonded to glucose（F1）. CONCLUSIONS：Apigenin mainly exists in form of prototype drug in rats. The reduction happens

on 2，3-double bond by the intestinal bacteria，and the product of apigenin boned to glucuronic acid or glucose can be formed

when excreting in intestinal tract and rats in vivo，while the product of apigenin boned to sulphate can be formed only when excreting

in rats in vivo.