目的：建立测定复方亚麻酸软胶囊中大豆卵磷脂和γ-亚麻酸含量的方法。方法：采用高效液相色谱法测定制剂中大豆卵

磷脂的含量：色谱柱为Agilent TC-C18，流动相为甲醇-水（84 ∶16，V/V），流速为1.0 ml/min，检测波长为219 nm，柱温为30 ℃，进样量

为10 μl；采用气相色谱法测定制剂中γ-亚麻酸的含量：色谱柱为DBWAX柱，进样口温度为210 ℃，程序升温，载气为氦气，流速为

1 ml/min，分流比为1 ∶30，进样量为0.02 μl。结果：L-α-卵磷脂和γ-亚麻酸甲酯检测质量浓度线性范围分别为4.64～16.24 μg/ml、

0.093 44～0.327 04 μg/ml（r＝0.999 9、0.999 8）；精密度、稳定性、重复性试验的RSD＜3％；加样回收率分别为96.79％～99.36％

（RSD＝0.93％，n＝6）、96.63％～99.07％（RSD＝1.01％，n＝6）。结论：该两种方法操作简便、稳定、重复性好，可用于复方亚麻酸

软胶囊中大豆卵磷脂和γ-亚麻酸含量的测定。

OBJECTIVE：To establish a method for the contents determination of phospholipid and γ-linolenic acid in Compound

linolenic acid soft capsule. METHODS：HPLC was conducted to determine the content of soybean lecithin；the column was

Agilent TC-C18 with mobile phase of methanol-water（84 ∶16，V/V）at a flow rate of 1.0 ml/min，the detection wavelength was 219

nm，the column temperature was 30 ℃，and the injection volume was 10 μl.Gas chromatography was conducted to determine the

content of γ-linolenic acid in the preparation；the column was DB WAX，injector temperature was 210 ℃ by temperature programmed，

carrier gas was helium at a flow rate of 1 ml/min by split injection（split ratio of 1 ∶30），and the injection volume was

0.02 μl. RESULTS：The linear range was 4.64-16.24 μg/ml for phospholipid（r＝0.999 6）and 0.093 44-0.327 04 μg/ml for γ-linolenic

acid methy ester（r＝0.999 6）；RSDs of precision，stability and reproducibility tests were lower than 3％ ；recoveries were

97.44％-99.36％（RSD＝0.93％，n＝6）and 97.22％-99.07％（RSD＝1.01％，n＝6），respectively. CONCLUSIONS：The method is

simple，stable with good separation，and can be use for the contents determination of phospholipid and γ-linolenic acid in Compound

linolenic acid soft capsule.