目的：建立同时测定儿泻停糖浆中葛根素、盐酸小檗碱、黄芩苷含量的方法。方法：采用高效液相色谱法。色谱柱为

Kromasil C18，流动相为甲醇-0.2％磷酸水溶液（梯度洗脱），流速为1.0 ml/min，柱温为30 ℃，检测波长为275 nm，进样量为10 μl。

结果：黄芩苷、盐酸小檗碱、葛根素的检测质量浓度线性范围分别为10.43～83.44、10.71～85.68、12.58～100.64 μg/ml；精密度、稳

定性、重复性试验的RSD＜2％；加样回收率分别为98.25％～100.87％、99.41％～101.40％、98.13％～99.76％，RSD 分别为

0.92％、0.68％、0.57％（n＝9）。结论：该方法操作简便，结果可靠，可用于儿泻停糖浆的质量控制。

OBJECTIVE：To establish a method for simultaneous determination of puerarin，berberine hydrochloride and baicalin

in Erxieting syrup. METHODS：HPLC was performed on the column of Kromasil-C18 with mobile phase of methanol-0.2％

Phosphoric acid solution（gradient elution）at flow rate of 1.0 ml/min，column temperature was 30 ℃，detection wavelength was

275 nm，and the volume injection was 10 μl. RESULTS：The linear was 10.43-83.44 μg/ml for puerarin，10.71-85.68 μg/ml for berberine

hydrochloride and 12.58-100.64 μg/ml for baicalin（r≥0.999 3）；RSDs of precision，stability and reproduciblity tests were

lower than 2％；recoveries were 98.25％-100.87％（RSD＝0.92％，n＝6），99.41％-101.40％（RSD＝0.68％，n＝6）and 98.13％-99.76％

（RSD＝0.57％，n＝6），respectively. CONCLUSIONS：The method is simple，reliable，can be used for the quality control of Erxieting

syrup.