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芍药汤对湿热泄泻大鼠肠道微生态的影响
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| 篇名： | 芍药汤对湿热泄泻大鼠肠道微生态的影响 |
| TITLE： | Effects of Shaoyao decoction on intestinal microecology in rats with damp-heat diarrhea |
| 摘要： | 目的 探讨芍药汤对湿热泄泻大鼠肠道微生态的影响。方法将雄性SD大鼠随机分为生理组、模型组和芍药汤低、中、高剂量组（单次以生药量计分别为5.62、28.10、56.20g/kg），每组7只。除生理组外，其余组均采用高糖高脂饲料、高温高湿环境饲养联合白酒、大肠杆菌菌悬液灌胃的方法构建湿热泄泻大鼠模型。造模成功后第2天，芍药汤各剂量组大鼠分别灌胃相应药液，早晚各1次，连续3d。末次给药后，进行粪便宏基因组测序及生物学信息分析，并检测其肠道细胞色素P450（CYP）酶及肠道菌群代谢酶活性。结果各组大鼠肠道菌群的各项α多样性指标比较，差异均无统计学意义（P＞0.05）。各组大鼠肠道菌群组成相似，主要菌门包括厚壁菌门、放线菌门、疣微菌门等，核心优势菌属包括杜氏杆菌属、乳杆菌属、龙包茨氏菌属；低、中、高剂量芍药汤可不同程度逆转模型组大鼠肠道厚壁菌门、龙包茨氏菌属、双歧杆菌属、单球杆菌属等相对丰度的降低，以及变形菌门、疣微菌门、埃希菌属-志贺菌属、棒状杆菌属等相对丰度的升高（P＜0.05）。芍药汤各剂量组大鼠参与碳代谢、辅因子生物合成等途径的肠道菌群的相对丰度较高。与模型组比较，芍药汤各剂量组大鼠的氨苯酚、咪达唑仑代谢消除率，对硝基苯酚浓度（低剂量组除外）及其代谢率均显著升高（P＜0.05）。结论芍药汤可纠正湿热泄泻大鼠的肠道菌群紊乱，促进肠道CYP代谢酶和菌群代谢酶活性的恢复，从而改善肠道微生态。 |
| ABSTRACT： | OBJECTIVE To explore the impact of Shaoyao decoction on the intestinal microecology of rats with damp-heat diarrhea. METHODS Male SD rats were randomly divided into physiological group， model group， and Shaoyao decoction low-， medium- and high-dose groups （single dose of 5.62， 28.10 and 56.20 g/kg， by raw drugs）， with 7 rats in each group. Except for the physiological group， the remaining groups were all fed with high-sugar and high-fat diet， raised in a high-temperature and high- humidity environment， and administered white liquor and Escherichia coli suspension by gavage to establish a rat model of damp- heat diarrhea. On the second day after modeling， rats in each dosage group of Shaoyao decoction were administered the corresponding solution by gavage， once in the morning and once in the evening， for 3 consecutive days. After the last administration， fecal metagenomic sequencing analysis and biological information analysis were performed to detect the activities of intestinal cytochrome P450 （CYP） enzymes and intestinal flora metabolic enzymes. RESULTS There was no significant difference in the α diversity indexes of intestinal flora among the groups （P＞0.05）. The main phyla of rats in each group included Firmicutes， Actinobacteriota and Verrucomicrobiota， and the core dominant genera included Dubosiella， Lactobacillus and Romboutsia； low- ， medium- and high-dose of Shaoyao decoction could reverse the decrease in the relative abundance of Firmicutes， Romboutsia， Bifidobacterium and Monoglobus， as well as the increase in the relative abundance of Proteobacteria， Verrucomicrobiota， Escherichia-Shigella and Corynebacterium （P＜0.05）. Rats in each dosage group of Shaoyao decoction showed higher relative abundance of intestinal flora involved in carbon metabolism and cofactor biosynthesis pathways. Compared with the model group， the elimination rates of aminophenol and midazolam， as well as the concentration （except for the low-dose group） and metabolic rate of p-nitrophenol in rats of each dose group of Shaoyao decoction were significantly increased （P＜0.05）. CONCLUSIONS Shaoyao decoction can correct intestinal flora disorder in rats with damp-heat diarrhea， promote the recovery of intestinal CYP metabolic enzymes and intestinal flora metabolic enzymes， and thereby comprehensively improve intestinal microecology. Δ 基金项目 国家自然科学基金青年科学基金项目 （No. 82104726）；河南省软科学研究项目（No.252400410455） |
| 期刊： | 2025年第36卷第24期 |
| 作者： | 凌霄；张丽；张佳楠；李春晓；李学林 |
| AUTHORS： | LING Xiao，ZHANG Li，ZHANG Jianan，LI Chunxiao，LI Xuelin |
| 关键字： | 芍药汤；湿热泄泻；肠道菌群；细胞色素P450酶；肠道代谢酶；宏基因组学 |
| KEYWORDS： | Shaoyao decoction； damp-heat diarrhea； intestinal |
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