返回 
加入收藏
当归有机酸的低共熔溶剂提取工艺优化及机制研究
x
请在关注微信后，向客服人员索取文件
| 篇名： | 当归有机酸的低共熔溶剂提取工艺优化及机制研究 |
| TITLE： | Deep eutectic solvents-based extraction of organic acids from Angelica sinensis：process optimization and mechanistic insights |
| 摘要： | 目的 优化当归有机酸的低共熔溶剂（DESs）提取工艺，并进行表征、抗氧化活性评价和提取机制分析。方法采用基于片段活度系数的真实溶剂化模拟的类导体屏蔽模型（COSMO-SAC）筛选DESs种类；以总有机酸含量为响应值，通过单因素实验和Box-Behnken响应面法对提取工艺进行优化。以当归饮片和（或）当归甲醇提取物为参照，利用扫描电子显微镜、傅里叶变换红外光谱（FTIR）仪对产物进行表征，并进行1，1-二苯基-2-苦肼基（DPPH）、2，2′-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基清除能力检测。利用量子化学密度泛函理论分析DESs对阿魏酸、绿原酸的提取机制。结果与结论最适DESs为氧化胆碱-丙二醇；当归有机酸最优提取工艺为氯化胆碱-丙二醇摩尔比为1∶1、DESs含水量70%、料液比1∶10、加热温度57℃、加热搅拌时间8min。3次验证实验的总有机酸含量为2.92mg/g，与预测值（2.91mg/g）的相对误差为0.34%。与当归饮片、甲醇提取物相比，DESs提取物的团粒结构几乎消失，表面有类似肠壁的褶皱；与甲醇提取物相比，其FTIR特征峰强度及峰面积积分更高，对DPPH、ABTS自由基的清除能力均更强。DESs对当归有机酸的提取，是极性匹配、氢键作用及结构适配共同作用的结果。 |
| ABSTRACT： | OBJECTIVE To optimize the extraction process of organic acids from Angelica sinensis using deep eutectic solvents （DESs）， and conduct characterization， antioxidant activity evaluation， and extraction mechanism analysis. METHODS The conductor-like screening model for realistic solvation with segment activity coefficients （COSMO-SAC） was employed to screen the types of DESs. With total organic acid content as the response value， single-factor experiments and Box-Behnken response-surface methodology were used to optimize the extraction conditions. Using A. sinensis decoction pieces and/or A. sinensis methanol extract as references， scanning electron microscope and Fourier transform infrared spectrometer （FTIR） were applied to characterize the products. Additionally， the 1，1-diphenyl-2-picrylhydrazyl （DPPH） and 2，2′-azino-bis（3-ethylbenzothiazoline-6- sulfonic acid） （ABTS） free radical scavenging capacities were determined. Density functional theory （DFT） was used to analyze the extraction mechanism of ferulic acid and chlorogenic acid by the DESs. RESULTS & CONCLUSIONS The optimal DESs was choline chloride-propanediol. The optimal extraction conditions for organic acids from A. sinensis were as follows： choline chloride- propanediol molar ratio of 1∶1， DESs water content of 70%， solid-liquid ratio of 1∶10， heating temperature of 57 ℃， and heating and stirring time of 8 min. In three validation experiments， the total organic acid content was 2.92 mg/g， yielding a relative error of 0.34% compared to the predicted value （2.91 mg/g）. Compared with A. sinensis decoction pieces and methanol extracts， the agglomerated structure of the DESs extract powder almost disappeared， showing the presence of lamellar structures similar to those of the intestinal wall. Compared with the methanol extract， the DES extract exhibited higher FTIR characteristic peak intensity and peak area integration， as well as stronger scavenging capacities against DPPH and ABTS free radicals. The extraction of organic acids from A. sinensis by DESs is the result of the combined effects of polarity matching， hydrogen bonding， and structural adaptation. |
| 期刊： | 2025年第36卷第22期 |
| 作者： | 陈丽元；陈志松；钱加芬；陈新利；常伟宇；吴晖 |
| AUTHORS： | CHEN Liyuan，CHEN Zhisong，QIAN Jiafen，CHEN Xinli，CHANG Weiyu，WU Hui |
| 关键字： | 当归；低共熔溶剂；阿魏酸；绿原酸；COSMO-SAC；Box-Behnken响应面法；工艺优化 |
| KEYWORDS： | Angelica sinensis； deep eutectic solvents； |
| 阅读数： | 1 次 |
| 本月下载数： | 0 次 |
* 注：未经本站明确许可，任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作！
