返回 
加入收藏
当归-茯苓药对干预过敏性鼻炎的作用机制预测及验证
x
请在关注微信后，向客服人员索取文件
| 篇名： | 当归-茯苓药对干预过敏性鼻炎的作用机制预测及验证 |
| TITLE： | Prediction and verification of the mechanism of action of herb pair of Angelica sinensis-Poria cocos in intervening in allergic rhinitis |
| 摘要： | 目的 研究当归-茯苓药对干预过敏性鼻炎的作用机制。方法通过网络药理学预测当归-茯苓药对干预过敏性鼻炎的核心靶点。通过腹腔注射氢氧化铝与卵清蛋白的混合液建立过敏性鼻炎大鼠模型，将建模成功的大鼠随机分为模型组、氯雷他定组（阳性对照组，0.9mg/kg）和当归-茯苓药对低、中、高剂量组（3、6、12g/kg），每组10只；另取10只健康大鼠为正常组。各组大鼠灌胃相应药液或生理盐水，每日1次，连续30d。末次给药后，计算各组大鼠的行为学评分，检测血清中免疫球蛋白E（IgE）、组胺、白细胞介素4（IL-4）水平和鼻黏膜组织中IL-6、肿瘤坏死因子α（TNF-α）、前列腺素内过氧化物合成酶2（PTGS2）蛋白表达水平，观察大鼠的鼻黏膜组织病理形态变化。结果网络药理学分析发现，IL-6、TNF、PTGS2可能在当归-茯苓药对干预过敏性鼻炎中发挥关键作用，IL-17、TNF信号通路可能是当归-茯苓药对干预过敏性鼻炎的典型炎症信号通路。动物实验结果显示，与模型组比较，各给药组大鼠的行为学评分和IgE、组胺、IL-4水平以及IL-6、TNF-α、PTGS2蛋白表达水平均显著降低（P＜0.05），且当归-茯苓药对作用呈剂量依赖性（P＜0.05）。结论当归-茯苓药对可有效改善过敏性鼻炎大鼠症状，其机制可能与抑制IL-6、TNF-α、PTGS2表达有关。 |
| ABSTRACT： | OBJECTIVE To study the mechanism of action of herb pair of Angelica sinensis-Poria cocos in intervening in allergic rhinitis. METHODS The core targets of herb pair of A. sinensis-P. cocos in intervening in allergic rhinitis were predicted through network pharmacology. Allergic rhinitis model of rats was established by intraperitoneal injection of aluminum hydroxide and ovalbumin mixture， and the rats were randomly divided into model group， loratadine group （positive control group， 0.9 mg/kg）， and low-， medium-， high-dose groups of herb pair of A. sinensis-P. cocos （3， 6 and 12 g/kg）， with 10 rats in each group. Another 10 healthy rats were used as the normal group. Each group of rats was intragastrically administered the corresponding liquid or normal saline once a day for 30 consecutive days. After the last administration， the behavioral scores of each group of rats were calculated， the levels of immunoglobulin E （IgE）， histamine， interleukin-4 （IL-4） in serum， and the protein expression levels of IL-6， tumor necrosis factor-α （TNF-α）， and prostaglandin-endoperoxide synthase 2 （PTGS2） in nasal mucosa tissue were detected. The pathological morphology changes of nasal mucosa tissue were observed. RESULTS Network pharmacology analysis revealed that IL-6， TNF and PTGS2 might play a key role in the intervention of allergic rhinitis by herb pair of A. sinensis-P. cocos， and the IL-17 and TNF signaling pathways might be the typical inflammatory signaling pathways intervened by herb pair of A. sinensis-P. cocos in allergic rhinitis. The results of animal experiments showed that compared with the model group， the behavioral score， the levels of IgE， histamine and IL-4， and the protein expressions of IL-6， TNF-α and PTGS2 in each administration group were decreased significantly （P＜0.05）， and there was a dose-dependent relationship with the herb pair of A. sinensis-P. cocos （P＜ 0.05）. CONCLUSIONS The herb pair of A. sinensis-P. cocos can effectively alleviate the symptoms of rats with allergic rhinitis， the mechanism of which may be related to the inhibition of the expressions of IL-6， TNF-α and PTGS2. |
| 期刊： | 2025年第36卷第22期 |
| 作者： | 程静凯；朱珊；田新磊；胡文杰；史兴婵；乔淑楠 |
| AUTHORS： | CHENG Jingkai，ZHU Shan，TIAN Xinlei，HU Wenjie，SHI Xingchan，QIAO Shunan |
| 关键字： | 当归；茯苓；过敏性鼻炎；网络药理学；作用机制 |
| KEYWORDS： | Angelica sinensis； Poria cocos； allergic |
| 阅读数： | 0 次 |
| 本月下载数： | 0 次 |
* 注：未经本站明确许可，任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作！
