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八正散对慢性尿路感染大鼠的改善作用及机制研究
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| 篇名： | 八正散对慢性尿路感染大鼠的改善作用及机制研究 |
| TITLE： | Improvement effects and mechanism of Bazheng powder on chronic urinary tract infection in rats |
| 摘要： | 目的 探讨八正散对大肠埃希菌致大鼠慢性尿路感染（CUTI）的改善作用及机制。方法将大鼠分为正常对照组、模型组、左氧氟沙星组（45mg/kg）和八正散组（4.95g/kg），每组10只。除正常对照组外，其余各组大鼠经尿道向膀胱内注射大肠埃希菌菌液（1×108cfu/mL）构建CUTI模型。造模同时，各组大鼠灌胃相应药液或水，每天1次，连续4周。末次给药后，检测大鼠血常规（白细胞数量和淋巴细胞百分比）以及血清中炎症因子[白细胞介素1β（IL-1β）、IL-6、IL-8、肿瘤坏死因子α（TNF-α）]、免疫指标[CD4、CD8、分泌型免疫球蛋白A（SIgA）]、肾功能指标[胱抑素C（Cys-C）、α1-微球蛋白（α1-MG）、尿素、肌酐]水平；观察大鼠肾脏和膀胱组织的病理学形态；检测大鼠膀胱组织中Toll样受体4（TLR4）、核因子κB（NF-κB）、核苷酸结合结构域富含亮氨酸重复序列和含热蛋白结构域受体3（NLRP3）蛋白的表达水平。结果与正常对照组比较，模型组大鼠血清中IL-1β、IL-6、IL-8、TNF-α、CD8、Cys-C、α1-MG、尿素、肌酐水平以及膀胱组织中TLR4、NF-κB、NLRP3蛋白的表达水平均显著升高（P＜0.05），CD4、SIgA水平均显著降低（P＜0.05）；肾脏和膀胱组织均可见大量炎症细胞浸润等病理学改变。与模型组比较，八正散组大鼠上述定量指标均显著逆转（P＜0.05），肾脏和膀胱组织均未见明显炎症性病变。结论八正散可减轻CUTI大鼠炎症反应，增强其免疫功能，上述作用可能与抑制TLR4/NF-κB/NLRP3信号通路有关。 |
| ABSTRACT： | OBJECTIVE To investigate the improvement effects and its mechanism of Bazheng powder on chronic urinary tract infection （CUTI） induced by Escherichia coli in rats. METHODS The rats were divided into normal control group， model group， levofloxacin group （45 mg/kg） and Bazheng powder group （4.95 g/kg）， with 10 rats in each group. Except for the normal control group， other groups were administered an intravesical injection of Escherichia coli suspension （1×10⁸ cfu/mL） via the urethra to establish CUTI model； at the same time， rats in each group were administered the corresponding medicinal solution or water by gavage once a day for 4 consecutive weeks. After the last medication， blood routine tests （white blood cell count and lymphocyte percentage）， the levels of serum inflammatory factors [interleukin-1β （IL-1β）， IL-6， IL-8， tumor necrosis factor-α （TNF-α）]， and immune indicators [CD4， CD8， secretory immunoglobulin A （SIgA）]， renal function indicators [cystatin C （Cys-C）， α1- microglobulin （α1-MG）， urea and creatinine] were all determined； the pathological changes in renal and bladder tissues in rats were observed. The protein expressions of Toll-like receptor 4 （TLR4）， nuclear factor-κB （NF-κB）， and nucleotide-binding domain leucine-rich repeat and pyrin domain-containing receptor 3 （NLRP3） in rat bladder tissues were detected. RESULTS Compared with the normal control group， the levels of IL-1β， IL-6， IL-8， TNF-α， CD8， Cys-C， α1-MG， urea and creatinine in serum， as well as the protein expressions of TLR4， NF-κB and NLRP3 in bladder tissues， were significantly elevated in the model group （P＜0.05）. Conversely， the levels of CD4 and SIgA were significantly decreased （P＜0.05）. Pathological changes， such as extensive infiltration of inflammatory cells， were observed in both renal and bladder tissues. Compared with the model group， the above quantitative indicators in the Bazheng powder group were significantly improved （P＜0.05）， with no obvious inflammatory lesions observed in either renal or bladder tissues. CONCLUSIONS Bazheng powder can alleviate inflammatory reaction and improve the immune function of CUTI rats， and its mechanism may be related to the inhibition of TLR4/NF-κB/ NLRP3 signaling pathway. |
| 期刊： | 2025年第36卷第20期 |
| 作者： | 葸慧荣；李小凤；蒋晓磊；李靖；马正安；李喜香 |
| AUTHORS： | XI Huirong，LI Xiaofeng，JIANG Xiaolei，LI Jing，MA Zheng’an，LI Xixiang |
| 关键字： | 八正散；慢性尿路感染；炎症反应；免疫功能；大肠埃希菌；TLR4/NF-κB/NLRP3信号通路 |
| KEYWORDS： | Bazheng powder； chronic urinary tract infection； |
| 阅读数： | 1 次 |
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