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黄芪建中汤对腹泻型肠易激综合征大鼠肠道炎症的影响及机制
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| 篇名： | 黄芪建中汤对腹泻型肠易激综合征大鼠肠道炎症的影响及机制 |
| TITLE： | Effects and mechanism of Huangqi jianzhong decoction on intestinal inflammation in the rats of irritable bowel syndrome with diarrhea |
| 摘要： | 目的 基于促分裂原活化的蛋白激酶（MAPK）/核因子κB（NF-κB）信号通路探讨黄芪建中汤对腹泻型肠易激综合征（IBS-D）大鼠肠道炎症的影响及潜在机制。方法取雄性SD大鼠，随机选取10只作为对照组，剩余大鼠采用乙酸灌肠+束缚应激的方式制备IBS-D大鼠模型。将造模成功的大鼠随机分为IBS-D组、中药组（黄芪建中汤15g/kg）、阳性对照药组（利福昔明片150mg/kg）组、激活剂组（p38MAPK激活剂anisomycin125μg/kg）、中药+激活剂组（黄芪建中汤15g/kg+anisomycin125μg/kg），每组10只。各组大鼠灌胃或尾静脉注射相应药液或等体积生理盐水，每天1次，连续2周。末次给药后，收集其24h粪便，进行粪便含水量计算及粪便性状评分评估，并检测最小容量阈值；检测血清中肿瘤坏死因子α（TNF-α）、白细胞介素1β（IL-1β）、IL-6水平；观察结肠组织病理学变化；检测结肠组织中紧密连接蛋白ZO-1、闭合蛋白（occludin）mRNA及MAPK/NF-κB信号通路相关蛋白的表达情况。结果与对照组相比，IBS-D组大鼠结肠组织可见明显水肿和炎症细胞浸润，其粪便含水量及性状评分，血清炎症因子水平，以及结肠组织中p38MAPK、NF-κBp65蛋白的磷酸化水平均显著升高，最小容量阈值和ZO-1、occludinmRNA的表达均显著降低或下调（P＜0.05）；与IBS-D组相比，中药组和阳性对照药组大鼠结肠组织病理学改变有所缓解，上述指标均显著好转（P＜0.05），而激活剂组上述指标均进一步恶化（P＜0.05）；与中药组相比，中药+激活剂组大鼠各定量指标均被显著逆转（P＜0.05）。结论黄芪建中汤可减轻IBS-D大鼠的结肠炎症，缓解内脏高敏性，对其肠道屏障功能具有一定的保护作用；上述作用可能与抑制MAPK/NF-κB信号通路有关。 |
| ABSTRACT： | OBJECTIVE To explore the effects and potential mechanism of Huangqi jianzhong decoction on intestinal inflammation in the rats of irritable bowel syndrome with diarrhea （IBS-D） based on the mitogen-activated protein kinase （MAPK）/ nuclear factor-κB （NF-κB） signaling pathway. METHODS Male SD rats were selected. Ten rats were randomly chosen as the control group， and the remaining rats （50 rats） were used to prepare the IBS-D model by acetic acid enema+restraint stress. The rats with successful modeling were randomly divided into the IBS-D group， the traditional Chinese medicine （TCM） group （Huangqi jianzhong decoction 15 g/kg）， the positive control drug group （Rifaximin tablets 150 mg/kg）， the activator group （anisomycin 125 μg/kg， the activator of p38 MAPK）， and the TCM+activator group （Huangqi jianzhong decoction 15 g/kg+ anisomycin 125 μg/kg）， with 10 rats in each group. Rats in each group were given a gavage or tail vein injection of the corresponding medicine liquid or the same volume of normal saline， once a day for two consecutive weeks. After the last administration， feces within 24 hours were collected for the calculation of fecal water content and fecal trait score， and the minimum volume threshold was detected. The levels of tumor necrosis factor-α （TNF-α）， interleukin-1β （IL-1β）， and IL-6 in serum were detected； the pathological changes of colonic tissue were observed； the expressions of tight junction protein ZO-1， occludin mRNAs and proteins related to the MAPK/NF- κB signaling pathway in colonic tissue were determined. RESULTS Compared with the control group， obvious edema and inflammatory cell infiltration could be observed in the colonic tissue of rats in the IBS-D group. The fecal water content and trait score， serum levels of inflammatory factors， as well as the protein phosphorylation levels of p38 MAPK and NF-κB p65 in the colonic tissue were significantly increased， while the minimum volume threshold and the mRNA expressions of ZO-1 and occludin were significantly decreased or down-regulated （P＜0.05）. Compared with the IBS-D group， the pathological changes of colonic tissue in the TCM group and positive control drug group were alleviated， and the above indicators improved significantly （P＜0.05）， while the above indicators in the activator group deteriorated further （P＜0.05）. Compared with the TCM group， the above indicators in the TCM+activator group were significantly reversed （P＜0.05）. CONCLUSIONS Huangqi jianzhong decoction can alleviate colonic inflammation in IBS-D rats， relieve visceral hypersensitivity， and has a certain protective effect on their intestinal barrier function. The above-mentioned effects may be related to the inhibition of the MAPK/NF- κB signaling pathway. |
| 期刊： | 2025年第36卷第18期 |
| 作者： | 孟欣茹；孟娜；赵莉；刘惠瑾 |
| AUTHORS： | MENG Xinru，MENG Na，ZHAO Li，LIU Huijin |
| 关键字： | 黄芪建中汤；腹泻型肠易激综合征；肠道炎症；MAPK/NF-κB信号通路 |
| KEYWORDS： | Huangqi jianzhong decoction； irritable bowel syndrome with diarrhea； intestinal inflammation； MAPK/NF-κB |
| 阅读数： | 8 次 |
| 本月下载数： | 0 次 |
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