基于HPLC指纹图谱和有效成分含量评价不同产地草豆蔻质量
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篇名: | 基于HPLC指纹图谱和有效成分含量评价不同产地草豆蔻质量 |
TITLE: | Evaluation of the quality of Alpinia katsumadai from different habitats based on HPLC fingerprint and determina-tion of active ingredient content |
摘要: | 目的 评价不同产地草豆蔻药材的质量差异。方法应用高效液相色谱(HPLC)法建立18批不同产地草豆蔻药材的指纹图谱,通过相似度评价、聚类分析(CA)、主成分分析(PCA)、正交偏最小二乘法判别分析(OPLS-DA),以及药材中山姜素、乔松素、小豆蔻明、桤木酮的含量测定结果来综合评价不同产地草豆蔻药材的质量。结果18批草豆蔻药材HPLC指纹图谱的相似度均>0.9;从中标定出了11个共有峰,指认了其中4个。CA与PCA均将18批草豆蔻药材聚为3类,提取了2个主成分(累计方差贡献率达89.798%);OPLS-DA筛选出了9个质量差异标志物,分别为4、9、3、2、7(乔松素)、8(小豆蔻明)、6(山姜素)、10、11(桤木酮)号峰对应的成分。18批样品中山姜素、乔松素、小豆蔻明、桤木酮的含量分别为4.5071~11.5797、5.1544~14.1833、5.1095~13.5883、4.4946~11.2772mg/g。结论不同产地草豆蔻药材的质量差异较大,其中以海南产草豆蔻药材质量最佳。 |
ABSTRACT: | OBJECTIVE To evaluate the quality differences of Alpinia katsumadai from different habitats. METHODS High- performance liquid chromatography(HPLC) was used to establish the fingerprints of A. katsumadai from 18 batches of different habitats, and the quality of A. katsumadai from different habitats was comprehensively evaluated by similarity evaluation, cluster analysis (CA), principal component analysis (PCA), orthogonal partial least squares-discriminant analysis (OPLS-DA) and the content determination results of alpinetin, pinocembrin, cardamonin and alnustone in A. katsumadai. RESULTS The similarity of HPLC fingerprints for 18 batches of A. katsumadai was >0.9. Eleven common peaks were identified from the chromatogram, and four of them were specifically characterized. Both CA and PCA grouped 18 batches of A. katsumadai into 3 categories, extracting 2 principal components (the cumulative variance contribution rate reached 89.798%). OPLS-DA identified 9 quality difference markers, namely the components corresponding to peaks 4, 9, 3, 2, 7 (pinocembrin), 8 (cardamonin), 6 (alpinetin), 10 and 11 (alnustone). The content of alpinetin, pinocembrin, cardamonin, and alnustone ranged from 4.507 1-11.579 7, 5.154 4-14.183 3, 5.109 5-13.588 3 and 4.494 6-11.277 2 mg/g, respectively. CONCLUSIONS The quality of A. katsumadai from different habitats is quite different, and the quality of A. katsumadai from Hainan is the best. |
期刊: | 2025年第36卷第18期 |
作者: | 廖延武;汪英俊;刘顺;邱明鸣;朱育凤 |
AUTHORS: | LIAO Yanwu,WANG Yingjun,LIU Shun,QIU Mingming,ZHU Yufeng |
关键字: | 草豆蔻;HPLC指纹图谱;化学模式识别;含量测定;产地;质量控制 |
KEYWORDS: | Alpinia katsumadai; HPLC fingerprint; chemical pattern recognition; content determination; habitats; quality control |
阅读数: | 6 次 |
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