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白花丹素对AECOPD大鼠炎症反应及氧化应激的影响及机制
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| 篇名： | 白花丹素对AECOPD大鼠炎症反应及氧化应激的影响及机制 |
| TITLE： | Effects and mechanism of plumbagin on the inflammatory response and oxidative stress in rats with AECOPD |
| 摘要： | 目的 基于Notch1/GATA3信号通路探究白花丹素对慢性阻塞性肺疾病急性加重期（AECOPD）大鼠炎症反应及氧化应激的影响及潜在机制。方法随机选择10只大鼠，作为对照组；另取65只大鼠，通过吸入香烟烟雾+气管滴注内毒素+鼻腔给菌的方式构建AECOPD模型，并将造模成功的50只大鼠随机分为AECOPD组、白花丹素低剂量组（10mg/kg）、白花丹素高剂量组（50mg/kg）、阳性对照组（地塞米松0.09mg/kg）、白花丹素高剂量+Jagged1（Notch1激活剂）组（50mg/kg+25mg/kg），每组10只。各组大鼠灌胃和腹腔注射相应药液或生理盐水，每天1次，连续28d。末次给药后，检测各组大鼠的肺功能指标（呼气流量峰值、0.3s用力呼气容积与用力肺活量比值）、支气管肺泡灌洗液中的炎症细胞数（白细胞总数及淋巴细胞数、中性粒细胞数、巨噬细胞数）和炎症因子[白细胞介素6（IL-6）、IL-10、肿瘤坏死因子α（TNF-α）]水平以及肺组织中氧化应激指标[超氧化物歧化酶（SOD）、丙二醛（MDA）]含量，观察肺组织病理学变化并进行病理学评分，检测肺组织中黏蛋白5ac（Muc5ac）、Notch1、GATA3蛋白的表达情况。结果与对照组比较，AECOPD组大鼠肺泡壁结构严重受损，可见大量炎症细胞浸润，并伴有管壁增厚等病理学改变；肺功能指标、IL-10水平、SOD含量均显著降低；各炎症细胞数，IL-6、TNF-α水平，MDA含量，病理学评分，以及Muc5ac、Notch1、GATA3蛋白的表达均显著升高或上调（P＜0.05）；与AECOPD组比较，白花丹素各剂量组大鼠肺组织病理学变化均明显减轻，上述定量指标均显著改善，且高剂量组的改善更明显（P＜0.05）；Jagged1可显著逆转高剂量白花丹素对AECOPD大鼠肺损伤及相关指标的改善作用（P＜0.05）。结论白花丹素可抑制AECOPD大鼠肺部的炎症反应及氧化应激，减轻肺损伤，改善肺功能，上述作用可能与抑制Notch1/GATA3信号通路有关。 |
| ABSTRACT： | OBJECTIVE To explore the effects and potential mechanism of plumbagin on the inflammatory response and oxidative stress in rats with acute exacerbation of chronic obstructive pulmonary disease （AECOPD） based on Notch1/GATA3 signaling pathway. METHODS Ten rats were randomly selected as the control group； another 65 rats were used to establish the AECOPD model by inhaling cigarette smoke， intratracheal administration of endotoxin， and nasal inoculation of bacteria. The 50 successfully modeled rats were randomly divided into the AECOPD group， plumbagin low-dose group （10 mg/kg）， plumbagin high-dose group （50 mg/kg）， positive control group （dexamethasone 0.09 mg/kg）， and high-dose plumbagin+Jagged1 （Notch1 activator） group （50 mg/kg+25 mg/kg）， with 10 rats in each group. Each group was administrated intragastrically or intraperitoneally with the corresponding drug solution or normal saline， once a day for 28 consecutive days. After the last administration， the lung function indicators （peak expiratory flow， the ratio of forced expiratory volume in 0.3 seconds to forced vital capacity）， the number of inflammatory cells （white blood cells， lymphocytes， neutrophils， macrophages） in bronchoalveolar lavage fluid， the levels of inflammatory factors [interleukin-6 （IL-6）， IL-10， tumor necrosis factor-α （TNF-α）] in lung tissue， and the contents of oxidative stress indicators [superoxide dismutase （SOD）， malondialdehyde （MDA）] in lung tissue were all determined in each group； the pathological changes of lung tissue and the pathological scores， as well as protein expressions of mucin 5ac （Muc5ac）， Notch1 and GATA3 in lung tissue were also detected. RESULTS Compared with the control group， the lung tissue of the AECOPD group rats showed severe damage to the alveolar wall structure， with a large number of inflammatory cells infiltration and accompanied by pathological changes such as thickening of the airway wall； their lung function indicators， IL-10 level， and SOD content were significantly decreased； while the number of various inflammatory cells， IL-6 and TNF-α levels， MDA content， pathological score， as well as protein expressions of Muc5ac， Notch1 and GATA3 were significantly increased or upregulated （P＜0.05）. Compared with the AECOPD group， the pathological changes in the lung tissue of the rats in each plumbagin dose group were significantly alleviated， and the above quantitative indicators were significantly improved， and the improvement was more obvious in the plumbagin high- dose group （P＜0.05）. Jagged1 significantly reversed the protective effect of high-dose plumbagin on lung injury and related indicators in AECOPD rats （P＜0.05）. CONCLUSIONS Plumbagin can inhibit the inflammatory response and oxidative stress in the lungs of AECOPD rats， alleviate lung damage， and improve lung function. The above effects may be related to the inhibition of the Notch1/GATA3 signaling pathway. |
| 期刊： | 2025年第36卷第18期 |
| 作者： | 王亚茹；许佩佩；李士荣 |
| AUTHORS： | WANG Yaru，XU Peipei，LI Shirong |
| 关键字： | 白花丹素；慢性阻塞性肺疾病急性加重期；炎症反应；氧化应激；Notch1/GATA3信号通路 |
| KEYWORDS： | plumbagin； acute exacerbation of chronic obstructive pulmonary disease； inflammatory response； oxidative stress； |
| 阅读数： | 7 次 |
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