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枸杞多糖抑制口腔癌细胞增殖、迁移及免疫逃逸的机制研究
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| 篇名： | 枸杞多糖抑制口腔癌细胞增殖、迁移及免疫逃逸的机制研究 |
| TITLE： | Study on the mechanism of Lycium barbarum polysaccharides inhibiting the proliferation，migration and immune escape of oral cancer cells |
| 摘要： | 目的 探索枸杞多糖调节T细胞免疫球蛋白黏蛋白3（TIM3）/半乳糖凝集素9（Gal-9）信号通路对口腔癌细胞增殖、迁移和免疫逃逸的影响。方法将人口腔癌KB、CAL27细胞分为对照组、低浓度枸杞多糖组（200μg/mL）、高浓度枸杞多糖组（400μg/mL）、pcDNA-NC组（转染pcDNA-NC质粒）、pcDNA-TIM3组（转染pcDNA-TIM3质粒）、高浓度枸杞多糖+pcDNA-NC组（400μg/mL枸杞多糖+转染pcDNA-NC质粒）、高浓度枸杞多糖+pcDNA-TIM3组（400μg/mL枸杞多糖+转染pcDNA-TIM3质粒）。测定细胞的增殖、迁移、侵袭能力，T细胞杀伤率，细胞上清液中干扰素γ（IFN-γ）、白细胞介素2（IL-2）水平，以及细胞中TIM3、Gal-9mRNA表达，以及细胞中吲哚胺2，3-双加氧酶1（IDO1）、程序性死亡受体配体1（PD-L1）、TIM3、Gal-9蛋白表达。结果与对照组比较，低、高浓度枸杞多糖组2种细胞的克隆形成率、划痕愈合率、侵袭细胞数以及IDO1、PD-L1蛋白和TIM3、Gal-9mRNA及其蛋白表达水平均显著降低/减少（P＜0.05），增殖抑制率、T细胞杀伤率和IFN-γ、IL-2水平均显著升高（P＜0.05）；与对照组、pcDNA-NC组比较，pcDNA-TIM3组2种细胞的克隆形成率、划痕愈合率、侵袭细胞数以及IDO1、PD-L1蛋白和TIM3、Gal-9mRNA及其蛋白表达水平均显著升高/增加（P＜0.05），增殖抑制率、T细胞杀伤率和IFN-γ、IL-2水平均显著降低（P＜0.05）；与高浓度枸杞多糖组、高浓度枸杞多糖+pcDNA-NC组比较，高浓度枸杞多糖+pcDNA-TIM3组2种细胞的克隆形成率、划痕愈合率、侵袭细胞数以及IDO1、PD-L1蛋白和TIM3、Gal-9mRNA及其蛋白表达水平均显著升高/增加（P＜0.05），增殖抑制率、T细胞杀伤率和IFN-γ、IL-2水平均显著降低（P＜0.05）。结论枸杞多糖可能通过抑制TIM3/Gal-9信号通路来抑制口腔癌细胞增殖、迁移和免疫逃逸。 |
| ABSTRACT： | OBJECTIVE To explore the effects of Lycium barbarum polysaccharides on the proliferation， migration， and immune escape of oral cancer cells by regulating the T cell immunoglobulin and mucin domain-containing protein 3 （TIM3）/ galectin-9 （Gal-9） signaling pathway. METHODS Human oral cancer cells KB and CAL27 were assigned to control group， L. barbarum polysaccharides low-concentration group （200 μg/mL）， L. barbarum polysaccharides high-concentration group （400 μg/mL）， pcDNA-NC group （transfection of pcDNA-NC plasmid）， pcDNA-TIM3 group （transfection of pcDNA-TIM3 plasmid）， high concentration of L. barbarum polysaccharides+pcDNA-NC group （400 μg/mL L. barbarum polysaccharides + transfection of pcDNA-NC plasmid）， and high concentration of L. barbarum polysaccharides+pcDNA-TIM3 group （400 μg/mL L. barbarum polysaccharides + transfection of pcDNA-TIM3 plasmid）. The proliferation， migration and invasion abilities of the cells， T cell killing rate as well as the levels of interferon-γ （IFN-γ） and interleukin-2 （IL-2） in the cell supernatant were measured. mRNA expressions of TIM3 and Gal-9 and protein expressions of indoleamine 2，3-dioxygenase 1 （IDO1）， programmed death-ligand 1 （PD-L1）， TIM3 and Gal-9 in the cells were also determined. RESULTS Compared with control group， the clone formation rate， scratch healing rate， the number of invasive cells， protein expressions of IDO1 and PD-L1， mRNA and protein expressions of TIM3 and Gal-9 in both cell types of L. barbarum polysaccharide low- and high-concentration groups were decreased significantly （P＜0.05）， while the proliferation inhibition rate， T cell killing rate， and the levels of IFN-γ and IL-2 were significantly increased （P＜0.05）. Compared with control group and pcDNA-NC group， the clone formation rate， scratch healing rate， the number of invasive cells， and protein expressions of IDO1 and PD-L1， mRNA and protein expressions of TIM3 and Gal-9 in both cell types of the pcDNA-TIM3 group were all significantly increased （P＜0.05）， while the proliferation inhibition rate， T cell killing rate， IFN-γ and IL-2 levels were significantly decreased （P＜0.05）. Compared with L. barbarum polysaccharides high-concentration group and high concentration of L. barbarum polysaccharides+pcDNA-NC group， the clone formation rate， scratch healing rate， the number of invasive cells， and protein expressions of IDO1 and PD-L1， mRNA and protein expressions of TIM3 and Gal-9 in both cell types of high concentration of L. barbarum polysaccharides+pcDNA-TIM3 group were significantly increased （P＜0.05）， while the proliferation inhibition rate， T cell killing rate， and the levels of IFN-γ and IL-2 were significantly decreased （P＜0.05）. CONCLUSIONS L. barbarum polysaccharides may inhibit the proliferation， migration， and immune escape of oral cancer cells by suppressing TIM3/Gal-9 signaling pathway. |
| 期刊： | 2025年第36卷第17期 |
| 作者： | 李锦宇；徐晓雨；王钰卓 |
| AUTHORS： | LI Jinyu，XU Xiaoyu，WANG Yuzhuo |
| 关键字： | 枸杞多糖；口腔癌；T细胞免疫球蛋白黏蛋白3；半乳糖凝集素9；增殖；迁移；免疫逃逸 |
| KEYWORDS： | Lycium barbarum polysaccharides； TIM3； galectin-9； oral cancer； proliferation； migration； immune escape |
| 阅读数： | 6 次 |
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