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超氧化物歧化酶抑制AFP表达对肝癌细胞PLC/PRF/5恶性生物学行为的影响
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| 篇名： | 超氧化物歧化酶抑制AFP表达对肝癌细胞PLC/PRF/5恶性生物学行为的影响 |
| TITLE： | Effects of superoxide dismutase inhibition of AFP expression on the malignant biological behavior of PLC/PRF/5 liver cancer cells |
| 摘要： | 目的 探索超氧化物歧化酶（SOD）给药对肝癌细胞PLC/PRF/5恶性生物学行为的影响，并分析SOD与甲胎蛋白（AFP）表达量的相关性，以期为将SOD作为肝细胞癌用药靶向至AFP提供新思路。方法以人正常肝细胞L-02、AFP表达阴性的人肝癌细胞HLE、AFP表达阳性的人肝癌细胞PLC/PRF/5为实验细胞。采用Westernblot法和SOD活性检测试剂盒检测改变AFP表达前后L-02、HLE、PLC/PRF/5细胞中的AFP和SOD表达及SOD活性；采用细胞划痕实验和CCK-8实验检测不同浓度SOD[0（对照）、0.188、0.375、0.75、1.5、3U/mL]给药对PLC/PRF/5细胞迁移和增殖的影响；采用Westernblot法检测上调SOD表达对PLC/PRF/5细胞恶性生物学行为相关蛋白AFP、肉瘤病毒蛋白（Src）表达的影响。结果与L-02组和HLE组比较，PLC/PRF/5组细胞中的SOD1、SOD2表达量以及SOD活性均显著降低（P＜0.05）。下调PLC/PRF/5细胞中的AFP表达后，与PLC/PRF/5组比较，PLC/PRF/5-shAFP组（即低表达）细胞中的SOD1、SOD2表达量以及SOD活性均显著升高（P＜0.05）。SOD作用48h时，与对照组比较，0.375、0.75、1.5、3U/mLSOD组的PLC/PRF/5细胞划痕愈合率均显著降低（P＜0.05）；SOD作用72h时，与对照组比较，0.375、0.75、1.5U/mLSOD组的PLC/PRF/5细胞划痕愈合率显著降低（P＜0.05或P＜0.01）。与对照组比较，0.375、0.75、1.5、3U/mLSOD组的PLC/PRF/5细胞增殖率显著降低（P＜0.05或P＜0.01）。与上调SOD1、SOD2表达前的PLC/PRF/5组比较，PLC/PRF/5-oeSOD1组和PLC/PRF/5-oeSOD2组（即过表达）细胞中的AFP、Src表达量均显著降低（P＜0.05）。结论一定浓度的SOD可抑制PLC/PRF/5细胞的迁移和增殖等恶性行为，SOD的表达量和活性与AFP呈负相关。 |
| ABSTRACT： | OBJECTIVE To explore the effect of superoxide dismutase （SOD） administration on the malignant behavior of PLC/PRF/5 liver cancer cells， and analyze the correlation between SOD and alpha-fetoprotein （AFP） expression， to provide new ideas for targeting AFP with SOD as a drug for hepatocellular carcinoma. METHODS Normal human liver cells L-02， AFP- negative human liver cancer cells HLE， and AFP-positive human liver cancer cells PLC/PRF/5 were used as experimental cells. Western blot assay and SOD activity detection kit were used to detect the expression of AFP， SOD and activity of SOD in cells before and after changing AFP expression； the effects of different concentrations of SOD [0 （control）， 0.188， 0.375， 0.75， 1.5， 3 U/mL] administration on the migration and proliferation of PLC/PRF/5 cells were detected using cell scratch assay and CCK-8 assay. The effects of SOD overexpression on the expression of malignant biological behavior-related proteins AFP and sarcoma virus protein （Src） in PLC/PRF/5 cells were detected using Western blot. RESULTS Compared with L-02 group and HLE group， the expression levels of SOD1 and SOD2， and SOD activity in PLC/PRF/5 cells were significantly reduced （P＜0.05）. After down-regulating AFP expression in PLC/PRF/ 5 cells， compared with PLC/PRF/5 group， the expression levels of SOD1 and SOD2， as well as SOD activity， were significantly increased in the PLC/PRF/5-shAFP group （low-expression） （P＜0.05）. After 48 hours of SOD treatment， compared with control group， the scratch healing rates of PLC/PRF/5 cells in the 0.375， 0.75， 1.5 and 3 U/mL SOD groups were significantly reduced （P＜0.05）； after 72 hours of SOD treatment， compared with control group， the scratch healing rates of PLC/PRF/5 cells in the 0.375， 0.75， and 1.5 U/mL SOD groups were significantly reduced （P＜0.05 or P＜0.01）. Compared with control group， proliferation rates of PLC/PRF/5 cells were significantly reduced in the 0.375， 0.75， 1.5 and 3 U/mL SOD groups （P＜0.05 or P＜0.01）. Compared with the PLC/PRF/5 group before up-regulating SOD1 and SOD2 expression， the expression levels of AFP and Src in the PLC/PRF/5-oeSOD1 and PLC/PRF/5-oeSOD2 groups （over-expression） after up-regulating SOD1 and SOD2 expression were significantly reduced （P＜0.05）. CONCLUSIONS A certain concentration of SOD can inhibit malignant behavior such as migration and proliferation of PLC/PRF/5 cells， and the expression level and activity of SOD are negatively correlated with AFP. |
| 期刊： | 2025年第36卷第17期 |
| 作者： | 陈栘；陈宝莹；周育丽；徐海霞；曹钰；谷悦；朱明月；李孟森 |
| AUTHORS： | CHEN Yi，CHEN Baoying，ZHOU Yuli，XU Haixia，CAO Yu，GU Yue，ZHU Mingyue，LI Mengsen |
| 关键字： | 超氧化物歧化酶；甲胎蛋白；肝细胞癌；恶性生物学行为；抗癌药物 |
| KEYWORDS： | superoxide dismutase； alpha fetoprotein； hepatocellular carcinoma； malignant behavior； anti-cancer drugs |
| 阅读数： | 3 次 |
| 本月下载数： | 0 次 |
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