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负载盐酸万古霉素的聚多巴胺纳米粒的制备及其联合光热的抗菌作用研究
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| 篇名： | 负载盐酸万古霉素的聚多巴胺纳米粒的制备及其联合光热的抗菌作用研究 |
| TITLE： | Preparation of vancomycin hydrochloride-loaded polydopamine nanoparticles and their antimicrobial effect in combination with photothermal therapy |
| 摘要： | 目的 制备负载盐酸万古霉素（VH）的聚多巴胺（PDA）纳米粒（即VH@PDA纳米粒），并研究其联合光热的抗菌作用。方法以PDA为载体负载VH，制备VH@PDA纳米粒（同法制备PDA纳米粒）。采用激光粒度仪、透射电子显微镜、紫外-可见吸收光谱仪等对VH@PDA纳米粒进行表征，并测定该纳米粒的载药量和包封率。通过近红外激光照射考察该纳米粒的光热性能；以金黄色葡萄球菌为研究对象，采用琼脂平板法、活/死细菌染色法、结晶紫染色法考察该纳米粒联合光热的体外抗菌作用。以L929细胞为研究对象，考察不同质量浓度（0、3.125、6.25、12.5、25、50、100μg/mL）VH@PDA纳米粒对细胞活力的影响，以考察生物相容性。结果VH@PDA纳米粒成功负载VH，其粒径（约为300nm）均一、孔径明显，呈球形结构；载药量为11.34%，包封率为32.00%；光热转化效率为23.55%，且具有稳定的光热性能。联合光热的抗菌作用结果显示，当不以近红外激光照射处理时，VH、PDA纳米粒、VH@PDA纳米粒的抗菌效果不明显，而以近红外激光照射处理时，VH@PDA纳米粒表现出明显的抗菌效果。细胞实验结果显示，不同质量浓度VH@PDA纳米粒处理后，细胞存活率均高于80%。结论本研究成功制备VH@PDA纳米粒，该纳米粒具有稳定的光热性能，联合光热的抗菌作用明显，且具有良好的生物相容性。 |
| ABSTRACT： | OBJECTIVE To prepare vancomycin hydrochloride （VH）-loaded polydopamine （PDA） nanoparticles （VH@PDA nanoparticles）， and study their antimicrobial effect in combination with photothermal therapy. METHODS Using PDA as the carrier， VH was loaded to prepare VH@PDA nanoparticles （PDA nanoparticles were prepared using the same method）. The nanoparticles were characterized with laser particle size analyzer， transmission electron microscope， and UV visible absorption spectrometer； the drug loading capacity and encapsulation efficiency of the nanoparticles were determined. The near-infrared laser irradiation was adopted to determine their photothermal ability. Taking Staphylococcus aureus as the research object， the bactericidal properties of the nanoparticles in combination with photothermal therapy in vitro were clarified through plate colony coating method， live/dead staining analysis， crystal violet staining. Using L929 cells as the research object， the effects of VH@PDA nanoparticles at different mass concentrations （0， 3.125， 6.25， 12.5， 25， 50 and 100 μg/mL） on cell viability were investigated to assess their biocompatibility. RESULTS VH@PDA nanoparticles were successfully loaded with VH， exhibiting uniform particle size （approximately 300 nm）， distinct pore size， and a spherical structure. The drug loading capacity was 11.34%， the encapsulation efficiency was 32.00%， the photothermal conversion efficiency reached 23.55%， and they demonstrated stable photothermal performance. The antibacterial effect results of the combined photothermal therapy demonstrated that without near- infrared laser irradiation， the antibacterial effects of VH， PDA nanoparticles， and VH@PDA nanoparticles were not significant. However， when subjected to near-infrared laser irradiation， VH@PDA nanoparticles exhibited a pronounced antibacterial effect. The results of the cell experiments revealed that after treatment with VH@PDA nanoparticles at various mass concentrations， the cell viability rates remained above 80%. CONCLUSIONS VH@PDA nanoparticles are successfully prepared， which exhibit stable photothermal properties， significant antibacterial effects when combined with photothermal therapy， and good biocompatibility. |
| 期刊： | 2025年第36卷第15期 |
| 作者： | 潘梨花；刘斌；江霞云；周小鹏 |
| AUTHORS： | PAN Lihua，LIU Bin，JIANG Xiayun，ZHOU Xiaopeng |
| 关键字： | 万古霉素；聚多巴胺；纳米粒；光热治疗；抗菌；生物相容性 |
| KEYWORDS： | vancomycin； polydopamine； nanoparticles； photothermal therapy； antibacterial； biocompatibility |
| 阅读数： | 4 次 |
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