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健脾和胃方的指纹图谱、化学模式识别分析及多指标含量测定
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| 篇名： | 健脾和胃方的指纹图谱、化学模式识别分析及多指标含量测定 |
| TITLE： | Fingerprints，chemical pattern recognition analysis，and multi-index content determination of Jianpi hewei formula |
| 摘要： | 目的 建立健脾和胃方的高效液相色谱（HPLC）指纹图谱，并进行化学模式识别分析和其中7种成分的含量测定，为健脾和胃方的质量控制及后续制剂开发提供参考。方法以15批健脾和胃方标准汤剂为样品，采用《中药色谱指纹图谱相似度评价系统（2012版）》建立HPLC指纹图谱，并进行相似度评价、色谱峰的指认和归属分析。以15批样品的共有峰峰面积为变量，采用层次聚类分析、主成分分析、正交偏最小二乘-判别分析对样品进行化学模式识别分析。采用HPLC法测定15批样品中腺嘌呤、5-羟甲基糠醛、延胡索乙素、柚皮苷、脱氢紫堇碱、新橙皮苷、甘草酸的含量。结果15批样品的HPLC指纹图谱中共标定出19个共有峰，各批次样品的相似度均大于0.95。化学模式识别分析结果显示，15批样品共聚为3类，筛选出的差异性成分有3个[峰7（5-羟甲基糠醛）、峰17（新橙皮苷）和峰15（柚皮苷）]。7种成分在各自进样质量浓度范围内线性关系均良好（R2≥0.9994）；精密度、稳定性、重复性试验的RSD均小于2%（n＝6）；平均加样回收率为98.95%～103.81%，RSD为0.61%～2.75%（n＝6）；含量分别为0.031～0.106、0.267～0.824、0.089～0.144、1.344～2.091、0.089～0.178、1.328～2.028、0.040～0.150mg/g。结论所建立的HPLC指纹图谱及多指标成分含量测定方法准确、可靠，结合化学模式识别分析可用于健脾和胃方的质量控制。 |
| ABSTRACT： | OBJECTIVE To establish HPLC fingerprint for Jianpi hewei formula （JPHWF）， conduct chemical pattern recognition analysis， and determine the contents of seven components in the formula， aiming to provide a scientific basis for quality control and further research of JPHWF. METHODS Taking 15 batches of standard decoctions of JPHWF as samples， the HPLC fingerprint was established using the Similarity Evaluation System of TCM Chromatographic Fingerprint （2012 edition）. Subsequently， similarity evaluation， as well as identification and attribution analysis of chromatographic peaks， were conducted. Using the common peak areas from the 15 batches of samples as variables， chemical pattern recognition analyses were performed on the samples through hierarchical cluster analysis， principal component analysis， and orthogonal partial least squares-discriminant analysis. The contents of adenine， 5-hydroxymethylfurfural， tetrahydropalmatine， naringin， dehydrocorydaline， neohesperidin and glycyrrhizic acid in 15 batches of samples were determined by HPLC. RESULTS There were 19 common peaks in the characteristic chromatograms for 15 batches of samples with the similarities of more than 0.95. Results of chemical pattern recognition analysis showed that 15 batches of samples could be clustered into 3 categories， and 3 differential compounds were found [peak 7 （5- hydroxymethylfurfural）， peak 17 （neohesperidin）， and peak 15 （naringin）]. The 7 components were linearly good in the respective concentration ranges （R2≥0.999 4）； RSDs of precision， stability and repeatability tests were less than 2% （n＝6）； the average recovery rate of 98.95%-103.81%， RSD of 0.61%-2.75% （n＝6）； the contents of them were 0.031-0.106， 0.267-0.824， 0.089- 0.144， 1.344-2.091， 0.089-0.178， 1.328-2.028， 0.040-0.150 mg/g， respectively. CONCLUSIONS Established HPLC fingerprinting method coupled with multi-index content determination is validated to be accurate and reliable， and its combination with chemical pattern recognition analysis can be applied to the quality control of JPHWF. |
| 期刊： | 2025年第36卷第15期 |
| 作者： | 何东栋；宗慧；王重阳；宛娟娟；毛学璞；黄川生；王新春；王立萍 |
| AUTHORS： | HE Dongdong，ZONG Hui， WANG Chongyang，WAN Juanjuan，MAO Xuepu，HUANG Chuansheng，WANG Xinchun，WANG Liping |
| 关键字： | 健脾和胃方；指纹图谱；化学成分；含量测定；层次聚类分析；主成分分析；正交偏最小二乘-判别分析 |
| KEYWORDS： | Jianpi hewei formula； fingerprint； chemical |
| 阅读数： | 3 次 |
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