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三化汤的化学成分、入血原型成分及代谢成分分析
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| 篇名： | 三化汤的化学成分、入血原型成分及代谢成分分析 |
| TITLE： | Analysis of chemical components，prototype components migrating to the blood and metabolites of Sanhua decoction |
| 摘要： | 目的 研究三化汤的化学成分及其在大鼠体内的入血原型和代谢成分。方法将雄性Wistar大鼠分为给药组和空白组，每组6只。给药组大鼠每天早晚各灌胃13.3g/kg的三化汤冻干粉药液1次，空白组大鼠每天早晚各灌胃等体积生理盐水1次，连续3d。收集两组大鼠末次给药后的血浆样品，采用超高效液相色谱-四极杆飞行时间串联质谱（UHPLC-Q-TOF/MS）技术，结合自建天然产物高分辨质谱数据库和相关文献，对三化汤化学成分及入血原型成分、代谢产物进行鉴定。结果与结论从三化汤冻干粉样品中鉴定出69个成分，29个来源于药用大黄，16个成分来源于厚朴，22个成分来源于枳实，10个成分来源于羌活，其中3个成分（柠檬酸、L-酪氨酸、腺苷）在4味药材中均存在；另有1个成分（阿魏酰葡萄糖酸）尚需确定具体归属。共鉴定出43个入血原型成分，包括黄酮类、酚类、蒽醌类、苯丙素类、生物碱类、三萜类成分。共鉴定出61个代谢产物，以黄酮类、蒽醌类、苯丙素类成分为主；代谢途径包括去甲基化等Ⅰ相代谢反应和硫酸化、葡萄糖醛酸化等Ⅱ相代谢反应。 |
| ABSTRACT： | OBJECTIVE To study the chemical components， components migrating to the blood and metabolites of Sanhua decoction in rats. METHODS Male Wistar rats were divided into administration group and blank group， with 6 rats in each group. The rats in the administration group were given 13.3 g/kg of Sanhua decoction lyophilized powder solution by gavage once a day in the morning and evening， and the rats in the blank group were given an equal volume of saline by gavage once a day in the morning and evening， both for 3 consecutive days. Plasma samples were collected from the two groups of rats after the last administration. The chemical components， prototype components migrating to the blood and metabolites of Sanhua decoction were analyzed by UHPLC-Q-TOF/MS technique. The structures were identified combined with the self-built natural product high- resolution mass spectrometry database and relevant literature. RESULTS & CONCLUSIONS Totally 69 compounds were identified from the lyophilized powder of Sanhua decoction， including 29 components from Rheum officinale， 16 components from Magnoliae Officinalis Cortex， 22 components from Aurantii Fructus Immaturus， and 10 components from Notopterygii Rhizoma et Radix. Among them， 3 components （citric acid， L-tyrosine， adenosine） were present in all 4 medicinal herbs. Another component （feruloylgluconic acid） still needed to be specifically attributed. A total of 43 prototype components migrating to the blood were identified， including flavonoids， phenols， anthraquinones， phenylpropanoids， alkaloids and triterpenoids. A total of 61 metabolites were identified， predominantly consisting of flavonoids， anthraquinones and phenylpropanoids. The metabolic pathways mainly involved phase Ⅰ metabolic reactions such as demethylation and phase Ⅱ metabolic reactions like sulfation and glucuronidation. |
| 期刊： | 2025年第36卷第14期 |
| 作者： | 刘艳平；陈业；陆涛；陶娟敏；邱彦 |
| AUTHORS： | LIU Yanping，CHEN Ye，LU Tao，TAO Juanmin，QIU Yan |
| 关键字： | 三化汤；化学成分；入血原型成分；代谢产物；UHPLC-Q-TOF/MS技术 |
| KEYWORDS： | Sanhua decoction； chemical components； prototype components migrating to the blood； metabolites； UHPLC-Q- |
| 阅读数： | 3 次 |
| 本月下载数： | 0 次 |
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