补肾宁神膏的指纹图谱建立、含量测定及体外抗氧化作用的谱效关系研究
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篇名: | 补肾宁神膏的指纹图谱建立、含量测定及体外抗氧化作用的谱效关系研究 |
TITLE: | Study on fingerprint establishment,content determination,and spectrum-effect relationship of in vitro antioxidant activity for Bushen ningshen ointment |
摘要: | 目的 建立补肾宁神膏的指纹图谱,测定其指标成分的含量,并考察其体外抗氧化活性。方法建立10批补肾宁神膏的高效液相色谱(HPLC)指纹图谱,并进行相似度评价和共有峰指认;同法测定其中红景天苷等10个成分的含量;以2,2′-联氨(-3-乙基苯并噻唑啉-6-磺酸)二氨盐(ABTS)和1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除率以及铁离子还原能力(FRAP)为指标考察其抗氧化活性,采用SIMCA14.1软件进行灰色关联度分析和偏最小二乘回归分析以建立谱效关系。结果10批补肾宁神膏共有24个共有峰,相似度均大于0.96;指认了11个共有峰,分别为腺苷(峰1)、红景天苷(峰4)、莫诺苷(峰6)、儿茶素(峰7)、芍药苷(峰10)、斯皮诺素(峰11)、阿魏酸(峰12)、异槲皮苷(峰13)、毛蕊花糖苷(峰14)、丹皮酚(峰23)及大黄素(峰24)。含量测定结果显示,红景天苷、莫诺苷、儿茶素、芍药苷、斯皮诺素、阿魏酸、异槲皮苷、毛蕊花糖苷、丹皮酚及大黄素的平均含量分别为0.725、1.962、0.214、3.395、0.124、0.107、0.286、0.019、0.034、0.067mg/g。10批补肾宁神膏的平均综合指数为85.08%~96.35%。谱效关系分析结果显示,24个共有峰与其抗氧化能力均呈正相关,其中17个共有峰的变量重要性投影值>1,分别为峰2、5~7、9、10、13~21、23、24。结论本研究成功建立了补肾宁神膏的HPLC指纹图谱及含量测定方法;莫诺苷等17个共有峰代表的化合物可能是其发挥抗氧化作用的药效成分。 |
ABSTRACT: | OBJECTIVE To establish the fingerprint of Bushen ningshen ointment, determine the contents of its major constituents, and investigate its in vitro antioxidant activity. METHODS High performance liquid chromatography (HPLC) fingerprints of 10 batches of Bushen ningshen ointment were established. Similarity evaluation and identification of common peaks were subsequently performed. The contents of 10 components such as salidroside were determined using the same HPLC method. Using the scavenging rates against 2,2′-azino bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 1,1-diphenyl-2- picrylhydrazyl (DPPH) radicals, as well as ferric ion reducing antioxidant power (FRAP) as indicators, the anti-oxidant activity of the ointment was evaluated; grey relational analysis and partial least squares regression were conducted using SIMCA 14.1 software to establish the spectrum-effect relationship. RESULTS The fingerprint chromatogram of 10 batches of Bushen ningshen ointment contained 24 common peaks, with similarity values all exceeding 0.96. Eleven peaks were identified as adenosine (peak 1), salidroside (peak 4), morroniside (peak 6), catechin (peak 7), paeoniflorin (peak 10), spinosin (peak 11), ferulic acid (peak 12), isoquercitrin (peak 13), E-mail:wli1743@163.com verbascoside (peak 14), paeonol (peak 23), and emodin (peak 24). Content determination results showed that the average contents of salidroside, morroniside, catechin, paeoniflorin, spinosin, ferulic acid, isoquercitrin, verbascoside, paeonol, and emodin were 0.725, 1.962, 0.214, 3.395, 0.124, 0.107, 0.286, 0.019, 0.034 and 0.067 mg/g, respectively. The antioxidant potency composite index (APC) for the 10 batches ranged from 85.08% to 96.35%. Spectrum-effect relationship analysis indicated that all 24 common peaks were positively correlated with the antioxidant capacity. Seventeen peaks had variable importance in projection values >1, specitically peaks 2, 5, 6, 7, 9, 10, 13- 21, 23, and 24. CONCLUSIONS This study successfully established the HPLC fingerprint and content determination method for Bushen ningshen ointment. The compounds represented by the 17 common peaks such as morroniside may be the active components contributing to its antioxidant effects. |
期刊: | 2025年第36卷第14期 |
作者: | 王丽;徐文华;杨洋;辛灵怡;张蓁怡;杨光义 |
AUTHORS: | WANG Li,XU Wenhua,YANG Yang,XIN Lingyi,ZHANG Zhenyi,YANG Guangyi |
关键字: | 补肾宁神膏;指纹图谱;谱效关系;抗氧化活性;含量测定 |
KEYWORDS: | Bushen ningshen ointment; fingerprint; spectrum-effect relationship; antioxidant activity; content determination |
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