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指纹图谱结合药效成分含量综合评价银丹活血通瘀颗粒质量
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| 篇名： | 指纹图谱结合药效成分含量综合评价银丹活血通瘀颗粒质量 |
| TITLE： | Comprehensive evaluation of the quality of Yindan huoxue tongyu granules based on fingerprint combined with content of pharmacodynamic components |
| 摘要： | 目的 评价银丹活血通瘀颗粒的质量。方法采用高效液相色谱-紫外-蒸发光散射联用技术建立15批银丹活血通瘀颗粒的指纹图谱，利用《中药色谱指纹图谱相似度评价系统》（2012版）进行相似度评价并确定共有峰；通过与对照品指纹图谱和单味饮片图谱比对，指认共有峰。运用网络药理学筛选指认成分的作用靶点和通路，构建“成分-靶点-通路”网络图，预测银丹活血通瘀颗粒的药效成分，并以同样方法测定药效成分的含量。结果15批样品的指纹图谱中共标定40个共有峰，指认出丹酚酸B、黄芪甲苷、三七皂苷R1、银杏内酯A等17个成分。网络药理学预测出17个成分主要通过作用于97个靶点和137条通路发挥药效作用。15批样品中毛蕊异黄酮葡萄糖苷、迷迭香酸、芒柄花苷、紫草酸、丹酚酸B、芒柄花素、人参皂苷Rb1、人参皂苷Rd、银杏内酯C、银杏内酯A、银杏内酯B、三七皂苷R1和黄芪甲苷的平均含量分别为0.1268、0.2320、0.0738、0.3532、3.6202、0.1910、0.3333、0.3174、0.7850、0.5382、0.4600、2.4751、0.3477mg/g。结论所建银丹活血通瘀颗粒的指纹图谱可反映制剂整体特点，结合网络药理学建立的其药效成分的含量测定方法准确可靠、重复性好，可用于评价银丹活血通瘀颗粒的质量。 |
| ABSTRACT： | OBJECTIVE To evaluate the quality of Yindan huoxue tongyu granules. METHODS Taking high performance liquid chromatography with ultraviolet and evaporative light scattering detection as method， the fingerprint of 15 batches of Yindan huoxue tongyu granules was established， and similarity evaluation was performed by Similarity Evaluation System of Chromatographic Fingerprint of TCM （2012 edition） to determine common peaks. The common peaks were identified by comparing with reference substance chromatograms and single decoction piece chromatograms. Network pharmacology was used to screen out core targets and pathways of identified components， construct a “component-target-pathway” network diagram， and predict the pharmacodynamic components of Yindan huoxue tongyu granules， and the content determination of these components was carried out by the same method. RESULTS HPLC fingerprints of 15 batches of Yindan huoxue tongyu granules were characterized with 40 common peaks， and 17 components including salvianolic acid B， astragaloside Ⅳ， notoginsenoside R1， and ginkgolide A were identified. Network pharmacology predicted that 17 components mainly acted on 97 core targets and 137 pathways to exert their pharmacological effect. Average contents of 13 bioactive components in 15 batches of samples were 0.126 8， 0.232 0， 0.073 8， 0.353 2， 3.620 2， 0.191 0， 0.333 3， 0.317 4， 0.785 0， 0.538 2， 0.460 0， 2.475 1 and 0.347 7 mg/g， including calycosin-7-O-β-D-glucoside， rosmarinic acid， formononetin， lithospermic acid， salvianolic acid B， ononin， ginsenoside Rb1， ginsenoside Rd， ginkgolide C， ginkgolide A， ginkgolide B， notoginsenoside R1， and astragaloside Ⅳ. CONCLUSIONS The established fingerprint of Yindan huoxue tongyu granules can reflect the overall characteristics of the preparation. The content determination method for its pharmacodynamic components， developed in combination with network pharmacology， is accurate， reliable， and exhibits good repeatability， making it suitable for evaluating the quality of Yindan huoxue tongyu granules. |
| 期刊： | 2025年第36卷第14期 |
| 作者： | 白淑贤；王单单；吴作敏；于晓涛；董敬远；李叶宁 |
| AUTHORS： | BAI Shuxian，WANG Dandan，WU Zuomin，YU Xiaotao，DONG Jingyuan，LI Yening |
| 关键字： | 银丹活血通瘀颗粒；指纹图谱；含量测定；网络药理学；药效成分；质量评价 |
| KEYWORDS： | Yindan huoxue tongyu granules； fingerprint； content determination； network pharmacology； pharmacodynamic |
| 阅读数： | 5 次 |
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