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桔梗炒制工艺及其炒制前后对急性肺损伤小鼠的保护作用研究
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| 篇名： | 桔梗炒制工艺及其炒制前后对急性肺损伤小鼠的保护作用研究 |
| TITLE： | Study on stir-frying process of Platycodon grandiflorum and its protective effect on acute lung injury in mice before and after stir-frying |
| 摘要： | 目的 优化桔梗炒制工艺，并比较桔梗炒制前后对小鼠肺损伤的保护作用，初步探索炒桔梗“炮制增效”的特性。方法在单因素实验基础上，以炒制时间、炒制温度、翻动频率为因素，以外观性状评分、桔梗皂苷D含量和醇浸出物含量的综合评分为指标，设计Box-Behnken响应面实验优化桔梗炒制工艺，并进行验证。将小鼠分为空白对照组，模型组，地塞米松组（阳性对照药，5mg/kg），桔梗低、高剂量组（0.6、1.2g/kg），炒桔梗低、高剂量组（0.6、1.2g/kg），每组8只；每天给药1次，连续10d。末次给药后，采用脂多糖气管滴注法建立急性肺损伤模型，检测小鼠血清中白细胞介素6（IL-6）、IL-1β、肿瘤坏死因子α（TNF-α）和肺组织中超氧化物歧化酶（SOD）、髓过氧化物酶（MPO）、丙二醛（MDA）水平，计算肺干湿重比和胸腺指数，观察小鼠肺组织病理学变化。结果炒桔梗的最佳工艺为：炒制温度120℃，炒制时间12min，翻动频率19r/min；3批工艺验证得出的综合评分均大于97分，RSD小于3%（n＝3）。药效学实验结果显示，与空白对照组比较，模型组小鼠肺干湿重比和IL-6、IL-1β、TNF-α、MPO、MDA水平均显著升高（P＜0.01），胸腺指数和SOD水平均显著降低（P＜0.01），肺组织明显损伤；与模型组比较，各给药组小鼠上述指标均显著改善（P＜0.01），肺组织损伤明显减轻；且在相同给药剂量下，除肺干湿重比外，炒桔梗组小鼠上述指标较生品桔梗组改善程度更显著（P＜0.05），肺组织损伤进一步减轻。结论优化的炒制工艺稳定、可行；相同给药剂量下炒桔梗对急性肺损伤小鼠的保护效果优于生品桔梗。 |
| ABSTRACT： | OBJECTIVE To optimize the stir-frying process of Platycodon grandiflorum， compare the protective effect of P. grandiflorum on lung injury in mice before and after stir-frying， and preliminarily explore the characteristics of “enhanced efficacy through processing” of stir-frying P. grandiflorum. METHODS On the basis of single-factor experiment， the stir-frying time， temperature and frequency were taken as factors， and the comprehensive scores of appearance traits， platycodin D content and alcohol extract content were taken as indexes. Box-Behnken response surface experiment was designed to optimize the stir-frying process of P. grandiflorum and verify it. The mice were divided into blank control group， model group， dexamethasone group （positive control drug， 5 mg/kg）， P. grandiflorum low-dose and high-dose groups （0.6，1.2 g/kg）， fried P. grandiflorum low and high dose groups （0.6， 1.2 g/kg）， with 8 mice in each group. The treatment was given once a day for 10 consecutive days. After the last administration， acute lung injury model was established by intratracheal instillation of lipopolysaccharide. The levels of interleukin-6 （IL-6）， IL-1β， tumor necrosis factor-α （TNF-α） in serum and superoxide dismutase （SOD）， myeloperoxidase （MPO） and malondialdehyde （MDA） in lung tissue were detected， lung wet/dry weight ratio and thymus index were calculated， and the pathological changes of lung tissue were observed. RESULTS The optimum processing conditions were as follows：stir- frying temperature of 120 ℃， stir-frying time of 12 min， stir-frying frequency of 19 r/min； the comprehensive scores of the three batches of process verification were all greater than 97 points， RSD＜3% （n＝3）. The results of pharmacodynamic experiments showed that compared with blank control group， the lung dry-wet weight ratio as well as the levels of IL-6， IL-1β， TNF-α， MPO and MDA in the model group were significantly increased （P＜0.01）， the thymus index and SOD level were significantly decreased （P＜0.01）， and the lung tissue was significantly damaged. Compared with model group， above indexes of the mice in each administration group were significantly improved （P＜0.01）， and the lung tissue injury was significantly reduced. At the same dose， except for the lung dry-wet weight ratio， the above indexes of the mice in the stir-fried P. grandiflorum groups were significantly improved compared with P. grandiflorum 涵。E-mail：495758271@qq.com groups （P＜0.05）， and the lung tissue damage was further reduced. CONCLUSIONS The optimized stir-frying process is stable and feasible. The protective effect of stir-fried P.grandiflorum on acute lung injury in mice is better than that of raw products at the same dosage. |
| 期刊： | 2025年第36卷第13期 |
| 作者： | 韩啸天；王雷；王亚萍；杨娅玲；齐滨 |
| AUTHORS： | HAN Xiaotian，WANG Lei，WANG Yaping，YANG Yaling，QI Bin |
| 关键字： | 桔梗；炒桔梗；炒制工艺；肺损伤；药效学；炮制增效 |
| KEYWORDS： | Platycodon grandiflorum； fried Platycodon grandiflorum； stir-frying process； lung injury； pharmacodynamics； |
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