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虎杖在急性痛风性关节炎大鼠中的入血成分及代谢产物分析
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| 篇名： | 虎杖在急性痛风性关节炎大鼠中的入血成分及代谢产物分析 |
| TITLE： | Analysis of components migrating to blood and metabolites of Polygonum cuspidatum in rats with acute gouty arthritis |
| 摘要： | 目的 分析虎杖在急性痛风性关节炎（AGA）大鼠中的入血成分及代谢产物。方法将SD大鼠随机分为空白组、模型组和虎杖给药组（10g/kg，以生药量计），每组6只。除空白组外，其余各组大鼠通过注射氧嗪酸钾和尿酸钠复制AGA模型，同时灌胃相应药液或水，每天1次，连续10d。观察大鼠膝关节组织病理学形态；收集大鼠血清样品，采用超高效液相色谱串联四极杆静电场轨道阱质谱法分析虎杖的入血成分和代谢产物。结果经虎杖干预后，AGA大鼠膝关节滑膜组织病理形态明显改善，炎症细胞浸润和增生减少，蜂窝状结构完整。在正、负离子模式下，虎杖给药组大鼠血清中共检测出67个化学成分，包括25个原型成分和42个代谢产物，涉及的化合物类型有二苯乙烯类、蒽醌类、萘酚类、黄酮类等，涉及的代谢反应有甲基化、乙酰化、硫酸酯化、葡萄糖醛酸化，其中虎杖苷、白藜芦醇、大黄素等化合物均能以原型入血并发生体内代谢。结论虎杖苷、白藜芦醇、大黄素等化合物可能是虎杖抗AGA的活性成分。 |
| ABSTRACT： | OBJECTIVE To analyze the components migrating to blood and metabolites of Polygonum cuspidatum in rats with acute gouty arthritis （AGA）. METHODS SD rats were randomly divided into blank group， model group and P. cuspidatum group （10 g/kg， by raw material）， with 6 rats in each group. Except for blank group， AGA model was induced in the remaining groups by injecting potassium oxonate and sodium urate； meanwhile， they were administered corresponding drug solutions or water intragastrically， once a day， for 10 consecutive days. The histopathological morphology of the knee joint tissues in rats was observed；rat serum samples were collected， and the components migrating to blood and metabolites of P. cuspidatum were analyzed by using UPLC-Q-Exactive-Orbitrap-MS. RESULTS Following the intervention with P. cuspidatum， the histopathological morphology of the knee joint synovial tissue in AGA rats showed significant improvement， with reduced inflammatory cell infiltration and hyperplasia， and the preservation of the honeycomb-like structure integrity. In both positive and negative ion modes， a total of 67 chemical components were detected in the serum of rats from P. cuspidatum group， including 25 prototype components and 42 metabolites. The involved compound types encompassed stilbenes， anthraquinones， naphthols， and flavonoids， among others. The metabolic reactions identified included methylation， acetylation， sulfation， and glucuronidation. Notably， compounds such as polydatin， resveratrol and emodin were capable of entering the bloodstream in their prototype forms and undergoing in vivo metabolism. CONCLUSIONS Compounds such as polydatin， resveratrol and emodin are likely to be the active components responsible for the anti-AGA effects of P. cuspidatum. |
| 期刊： | 2025年第36卷第13期 |
| 作者： | 柯彩依；沈蒙；吉莉；王雪纯；朱雨晴；陈西；王铖维琦；马群 |
| AUTHORS： | KE Caiyi，SHEN Meng，JI Li，WANG Xuechun，ZHU Yuqing，CHEN Xi，WANG Chengweiqi，MA Qun |
| 关键字： | 虎杖；急性痛风性关节炎；入血成分；代谢产物；药效；活性成分；超高效液相色谱串联四极杆静电场轨道阱质谱法 |
| KEYWORDS： | Polygonum cuspidatum； acute gouty arthritis； components migrating to blood； metabolites； pharmacokinetics； |
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