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马齿苋乳膏对变应性接触性皮炎小鼠皮肤瘙痒及皮肤屏障功能的影响研究
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| 篇名： | 马齿苋乳膏对变应性接触性皮炎小鼠皮肤瘙痒及皮肤屏障功能的影响研究 |
| TITLE： | Effects of Portulaca oleracea cream on skin pruritus and barrier function in allergic contact dermatitis mice |
| 摘要： | 目的 研究马齿苋乳膏对变应性接触性皮炎（ACD）小鼠皮肤瘙痒及皮肤屏障功能的影响及机制。方法制备马齿苋提取液[1g/mL（以生药量计）]含量分别为10%、20%的马齿苋低、高浓度乳膏。将60只BALB/c小鼠随机分为空白组、模型组、糠酸莫米松乳膏组（阳性对照）、空白基质乳膏组、马齿苋低浓度乳膏组、马齿苋高浓度乳膏组。除空白组外，各组小鼠涂抹2，4-二硝基氯苯溶液诱导建立ACD模型；同时空白组及模型组小鼠涂抹生理盐水，其余各组小鼠涂抹相应药物，每天1次，每次约0.5g，连续14d。末次给药24h后，观察小鼠皮肤损伤情况并评分；观察小鼠皮肤组织病理学形态变化；检测小鼠血清中免疫球蛋白E（IgE）、肿瘤坏死因子α（TNF-α）水平；检测小鼠背根神经节（DRG）中MAS相关G蛋白偶联受体（包括MrgprA3、MrgprC11、MrgprD）mRNA的表达；检测小鼠皮肤组织中皮肤屏障功能相关蛋白Claudin-1、Occludin的表达。结果与空白组比较，模型组小鼠皮肤损伤严重，表皮结构缺失，皮肤组织角化过度，可见大量炎症细胞浸润；皮肤损伤评分，血清中IgE、TNF-α水平，DRG中MrgprA3、MrgprC11、MrgprDmRNA表达水平均显著升高（P＜0.05或P＜0.01），皮肤组织中Claudin-1、Occludin蛋白表达水平均显著降低（P＜0.05）。与模型组比较，马齿苋低、高浓度乳膏组小鼠皮肤损伤明显减轻，表皮增厚及真皮层海绵样变性缓解，炎症细胞浸润减轻，上述定量指标大部分显著逆转（P＜0.05或P＜0.01）；空白基质乳膏组大鼠上述皮肤损伤、病理学形态以及定量指标无显著差异。结论马齿苋能够改善ACD小鼠皮肤瘙痒，并修复皮肤屏障功能，其作用机制可能与下调小鼠DRG中MrgprA3、MrgprC11、MrgprDmRNA的表达，上调皮肤组织中Claudin-1、Occludin蛋白表达相关。 |
| ABSTRACT： | OBJECTIVE To study the effects and mechanism of Portulaca oleracea cream on skin pruritus and barrier function in allergic contact dermatitis （ACD） mice. METHODS Low-concentration and high-concentration P. oleracea creams were prepared， with the P. oleracea extract solution （1 g/mL， calculated by crude drug） concentrations of 10% and 20%. Sixty BALB/c mice were randomly allocated into blank group， model group， Mometasone furoate cream group （positive control）， blank matrix cream group， P. oleracea low-concentration and high-concentration cream groups. Except for blank group， ACD model was induced in each group using 2，4-dinitrochlorobenzene solution. The blank group and model groups received normal saline， while the remaining groups were treated with their respective creams， once a day， at a dose of approximately 0.5 g per application， continuously for 14 days. At 24 h post-final administration， skin lesions of mice were observed and scored； pathological changes of skin tissue were observed； serum levels of immunoglobulin E（IgE） and tumor necrosis factor-α （TNF-α） were quantified. mRNA expression of MAS-related G protein-coupled receptors （including MrgprA3， MrgprC11， and MrgprD） in dorsal root ganglion （DRG） was assessed； while protein expressions of skin barrier function-related proteins Claudin-1 and Occludin in skin tissues were determined. RESULTS Compared with blank group， mice in the model group exhibited severe skin damage， characterized by loss of epidermal architecture， hyperkeratosis of the skin tissue， and the infiltration of a large number of inflammatory cells. The skin injury scores， as well as the serum levels of IgE and TNF-α， and the mRNA expression levels of MrgprA3， MrgprC11， and MrgprD in DRG， were all significantly elevated compared to the blank group （P＜0.05 or P＜0.01）； in contrast， the protein expression levels of Claudin-1 and Occludin in the skin tissue were markedly reduced （P＜0.05）. Compared with model group， mice in P. oleracea low-concentration and high- concentration cream groups demonstrated significant alleviation of skin damage， as evidenced by reduced epidermal hyperplasia， mitigated spongiosis in the dermis， and decreased infiltration of inflammatory cells； these quantitative indicators were almost significantly reversed （P＜0.05 or P＜0.01）. No significant differences were observed in the aforementioned skin injuries， pathological alterations， or quantitative indicators between the blank matrix cream group and the model group. CONCLUSIONS P. oleracea may ameliorate skin lesions and restore skin barrier function of ACD mice， the mechanism of which may be associated with downregulating mRNA expressions of MrgprA3， MrgprC11 and MrgprD in DRG， and up-regulating the protein expressions of Claudin-1 and Occludin in skin tissue. |
| 期刊： | 2025年第36卷第11期 |
| 作者： | 王小雪；陈霞；蒲翔；樊官伟；孔祥艳；唐英；吴娜娜；骆江利 |
| AUTHORS： | WANG Xiaoxue，CHEN Xia，PU Xiang，FAN Guanwei，KONG Xiangyan，TANG Ying，WU Nana，LUO Jiangli |
| 关键字： | 马齿苋；乳膏；变应性接触性皮炎；瘙痒；皮肤屏障；炎症 |
| KEYWORDS： | Portulaca oleracea； cream； allergic contact dermatitis； pruritus； skin barrier； inflammation |
| 阅读数： | 14 次 |
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