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泽泻汤传统汤剂和配方颗粒降低脂质堆积的作用机制和药效研究
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| 篇名： | 泽泻汤传统汤剂和配方颗粒降低脂质堆积的作用机制和药效研究 |
| TITLE： | Comparative study on the mechanism and efficacy of Zexie tang traditional decoction and formula granules in reducing lipid accumulation |
| 摘要： | 目的 通过网络药理学探究泽泻汤降低脂质堆积的作用机制，并比较泽泻汤传统汤剂、配方颗粒降低脂质堆积的药效差异。方法采用TCMSP、SwissTargetPrediction数据库检索泽泻汤活性成分和靶点；采用GeneCards、OMIM、DisGeNET、TTD数据库分析非酒精性脂肪性肝病（NAFLD）相关靶点；采用String数据库构建交集靶点蛋白质-蛋白质相互作用网络模型；运用Cyto‐Scape软件构建“泽泻汤-NAFLD靶点-通路”网络图；利用Metascape平台进行靶点富集分析。建立人肝癌HepG2细胞脂质堆积模型，以吸光度值反映泽泻汤传统汤剂、配方颗粒对细胞脂质堆积的影响。结果泽泻汤治疗NAFLD的关键活性成分包括泽泻醇B、泽泻醇C、一亚油酸甘油酯、泽泻醇B单乙酸酯等，核心靶点包括MDM2、MAPK1、PIK3CB、PRKCQ、MAPK14等，核心信号通路包括内分泌抵抗、胰岛素抵抗、辅助性T细胞17细胞分化等。与模型组比，除泽泻配方颗粒组、白术配方颗粒组外，各给药组细胞吸光度值均显著降低（P＜0.01）；白术传统汤剂组吸光度值显著高于泽泻汤传统汤剂组（P＜0.01）；泽泻配方颗粒组、白术配方颗粒组吸光度值均显著高于泽泻汤配方颗粒组（P＜0.01）；泽泻配方颗粒组吸光度值显著高于泽泻传统汤剂组（P＜0.01）；白术配方颗粒组吸光度值显著高于白术传统汤剂组（P＜0.01）。结论泽泻汤通过多成分、多靶点、多通路降低人肝癌细胞脂质堆积，其传统汤剂与配方颗粒降脂效果略有差异。 |
| ABSTRACT： | OBJECTIVE To explore the effect and mechanism of Zexie tang （ZXT） on reducing lipid accumulation through network pharmacology， and compare the difference of traditional decoction versus formula granules. METHODS The active components and targets of ZXT were identified using TCMSP and SwissTargetPrediction databases. GeneCards， OMIM， DisGeNET and TTD databases were used to analyze the related targets of non-alcoholic fatty liver disease （NAFLD）； protein-protein interaction network model was constructed by String database；“ ZXT-NAFLD target-pathway” network diagram was constructed by using CytoScape software； target enrichment analysis was performed by using Metascape platform. Fat accumulation model of human hepatocellular carcinoma HepG2 cells was established to observe the effects of traditional decoction and formula granules of ZXT on lipid accumulation of cells. RESULTS Alisol B， alisol C， 1-monolinolein and alisol B monoacetate were the key active components of ZXT in the treatment of NAFLD. The core targets included MDM2， MAPK1， PIK3CB， PRKCQ and MAPK14， etc. The core signaling pathways included endocrine resistance， insulin resistance and Th17 cell differentiation. Compared with model group， except for the Zexie formula granules group and Baizhu formula granules group， the absorbance values in all other administration groups were significantly decreased （P＜0.01）； the absorbance value of Baizhu traditional decoction group was significantly higher than that of ZXT traditional decoction group （P＜0.01）； the absorbance values of Zexie formula granule group and Baizhu formula granule group were significantly higher than that of ZXT formula granule group （P＜0.01）； the absorbance value of Zexie formula granule group was significantly higher than that of Zexie traditional decoction group （P＜0.01）； the absorbance value of Baizhu formula granule group was significantly higher than that of Baizhu traditional decoction group （P＜0.01）. CONCLUSIONS ZXT reduces lipid accumulation of human hepatocellular carcinoma cells through multiple components， multiple target and multiple pathways， and its traditional decoction and formula granules exhibit slightly different lipid-lowering effects. |
| 期刊： | 2025年第36卷第10期 |
| 作者： | 郭媛媛；马丽娜；蔺虎琴；郑长辉；李佳怡；李旨君；曹俊岭 |
| AUTHORS： | GUO Yuanyuan，MA Lina，LIN Huqin，ZHENG Changhui，LI Jiayi，LI Zhijun，CAO Junling |
| 关键字： | 泽泻汤；传统汤剂；配方颗粒；非酒精性脂肪性肝病；人肝癌细胞；脂质堆积 |
| KEYWORDS： | Zexie tang； traditional decoction； formula granules； non-alcoholic fatty liver disease； human hepatocellular |
| 阅读数： | 4 次 |
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