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基于定性、定量及化学模式识别分析的蒙药森登-4质量评价
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| 篇名： | 基于定性、定量及化学模式识别分析的蒙药森登-4质量评价 |
| TITLE： | Quality evaluation of Mongolian medicine Sendeng-4 based on qualitative and quantitative analysis combined with chemical pattern recognition |
| 摘要： | 目的 基于定性、定量及化学模式识别分析对蒙药森登-4进行质量评价，以期为其质量控制提供参考。方法采用高效液相色谱-四极杆/静电场轨道阱高分辨质谱（HPLC-Q-Exactive-MS）技术对森登-4中化学成分进行定性分析；采用高效液相色谱串联质谱（HPLC-MS/MS）技术定量分析15批森登-4样品（S1～S15）中16个成分（没食子酸甲酯、没食子酸乙酯、表儿茶素、二氢杨梅素、京尼平-1-O-β-D-龙胆双糖苷、咖啡酸、儿茶素、柯里拉京、去乙酰车叶草苷酸甲酯、芦丁、栀子苷、木犀草素、杨梅素、槲皮素、阿魏酸、川楝素）的含量；对15批样品进行聚类分析、主成分分析、正交偏最小二乘-判别分析，并以变量重要性投影（VIP）值大于1为标准筛选差异性成分。结果在森登-4中共鉴定出73个化学成分，包括20个黄酮类、16个鞣质类、14个有机酸类成分等。定量分析结果显示，上述16个成分的含量分别为3.683～7.730、2.391～6.952、2275.538～4377.491、2699.188～3537.924、858.266～1377.393、3.366～11.003、140.624～315.683、414.629～978.334、285.501～1510.457、27.799～48.325、3625.415～6309.563、0.506～0.656、442.337～649.283、47.093～59.736、12.942～15.822、127.738～326.649μg/g。聚类分析和主成分分析结果显示，15批森登-4样品可聚为2类：S1～S3、S5～S6、S9～S10、S13样品聚为一类，S4、S7～S8、S11～S12、S14～S15样品聚为另一类；表儿茶素、京尼平-1-O-β-D-龙胆双糖苷、去乙酰车叶草苷酸甲酯、栀子苷的VIP值大于1。结论本研究采用HPLC-Q-Exactive-MS和HPLC-MS/MS技术对森登-4进行了定性、定量分析，并结合化学模式识别分析筛选出了栀子苷、表儿茶素、去乙酰车叶草苷酸甲酯、京尼平-1-O-β-D-龙胆双糖苷4个差异性成分。 |
| ABSTRACT： | OBJECTIVE To evaluate the quality of Mongolian medicine Sendeng-4 based on qualitative and quantitative analysis combined with chemical pattern recognition， in order to provide the reference for its quality control. METHODS The chemical components in Sendeng-4 were analyzed qualitatively by HPLC-Q-Exactive-MS. The contents of 16 components （methyl gallate， ethyl gallate， epicatechin， dihydromyricetin， genipin-1-O-β-D-gentiobioside， caffeic acid， catechin， corilagin， deacetylasperulosidic acid methyl ester， rutin， geniposide， luteolin， myricetin， quercetin， ferulic acid， and toosendanin） in 15 batches of Sendeng-4 （sample S1-S15） were quantitatively analyzed by HPLC-MS/MS. Cluster analysis （CA）， principal component analysis （PCA）， and orthogonal partial least squares discriminant analysis were conducted and variable importance projection （VIP） value greater than 1 was used as the index to screen the differential components. RESULTS A total of 73 chemical components were identified in Sendeng-4， including 20 flavonoids， 16 tannins， 14 organic acids， etc. According to the quantitative analysis， the results exhibited that the average contentsthe of above 16 components in 15 batches of Sendeng-4 were 3.683-7.730， 2.391-6.952， 2 275.538-4 377.491， 2 699.188-3 537.924， 858.266-1 377.393， 3.366-11.003， 140.624-315.683，414.629-978.334， 285.501-1 510.457， 27.799-48.325， 3 625.415-6 309.563， 0.506-0.656， 442.337-649.283， 47.093-59.736， 12.942-15.822， 127.738-326.649 μg/g， respectively. According to the results of CA and PCA， 15 batches of samples could be clustered into two categories: S1-S3， S5-S6， S9-S10 and S13 were clustered into one category； S4， S7-S8， S11-S12， S14-S15 were clustered into one category. VIP values of geniposide， epicatechin， deacetylasperulosidic acid methyl ester and genipin-1-O- β-D-gentiobioside were all greater than 1. CONCLUSIONS HPLC-Q-Exactive-MS and HPLC-MS/MS techniques are employed for the qualitative and quantitative analysis of Sendeng-4. Through chemical pattern recognition analysis， four differential components are identified: geniposide， epicatechin， deacetylasperulosidic acid methyl ester， and genipin-1-O-β-D-gentiobioside. |
| 期刊： | 2025年第36卷第09期 |
| 作者： | 周枫叶；李君；张谦；李荣杰；张微；刘静；王芳；李胜男 |
| AUTHORS： | ZHOU Fengye，LI Jun，ZHANG Qian，LI Rongjie，ZHANG Wei，LIU Jing，WANG Fang，LI Shengnan |
| 关键字： | 森登-4；定性分析；定量分析；化学模式识别分析；含量测定；质量评价；差异性成分 |
| KEYWORDS： | Sendeng-4； qualitative analysis； quantitative analysis； chemical pattern recognition analysis； content |
| 阅读数： | 7 次 |
| 本月下载数： | 0 次 |
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