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复方芩柏颗粒对溃疡性结肠炎大鼠的改善作用及机制研究
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| 篇名： | 复方芩柏颗粒对溃疡性结肠炎大鼠的改善作用及机制研究 |
| TITLE： | Study on the improvement effects of Compound qinbai granules on ulcerative colitis in rats and its mechanism |
| 摘要： | 目的 基于短链脂肪酸（SCFA）及其作用靶点G蛋白偶联受体（GPR），探究复方芩柏颗粒对溃疡性结肠炎（UC）大鼠的改善作用及潜在机制。方法将雄性SD大鼠随机分为正常组（12只）和模型制备组（30只），模型制备组大鼠通过饮用5%葡聚糖硫酸钠溶液构建UC模型。将造模成功的大鼠分为模型组、阳性对照组[美沙拉秦肠溶片270mg/（kg·d）]、复方芩柏颗粒组[2.52g/（kg·d）]，每组9只。各组大鼠每天分2次灌胃生理盐水或相应药液，连续3周。灌胃期间，观察各组大鼠一般情况，于末次给药后进行疾病活动性指数（DAI）评分，并检测其血清中促炎因子（肿瘤坏死因子α、白细胞介素6）和抑炎因子（转化生长因子β1、白细胞介素10）水平，观察其结肠组织病理变化并进行病理学评分，检测其粪便中SCFA（乙酸、丙酸、丁酸）含量以及结肠组织中GPR41、GPR43、GPR109A蛋白及mRNA的表达情况。结果与正常组比较，模型组大鼠精神萎靡，粪便带血明显，结肠组织结构受损严重，可见明显的腺体缺失、水肿、炎症细胞浸润等病理改变；其血清促炎因子水平、DAI评分、结肠病理学评分均显著升高，而抑炎因子水平，SCFA含量以及GPR41、GPR43、GPR109A蛋白及mRNA的表达均显著降低或下调（P＜0.01）。与模型组比较，各药物组大鼠一般情况及结肠组织病理改变均有好转，上述定量指标均明显逆转（P＜0.05或P＜0.01）。结论复方芩柏颗粒能减轻UC大鼠的肠道炎症及肠道黏膜病理损伤，上述作用可能与其恢复肠道SCFA的含量及其靶点GPR的表达有关。 |
| ABSTRACT： | OBJECTIVE To investigate the improvement effects of Compound qinbai granules on ulcerative colitis （UC） in rats and its mechanism based on short-chain fatty acid （SCFA） and their targets G protein-coupled receptor （GPR）. METHODS Male SD rats were randomly divided into normal group （12 rats） and model group （30 rats）； the model group was given 5% dextran sulfate sodium solution to induce the UC model. Model rats were divided into the model group， positive control group [Mesalazine enteric-coated tablets 270 mg/（kg·d）] and Compound qinbai granules group [2.52 g/（kg·d）]， with 9 rats in each group. Rats in each group were orally administered with normal saline or corresponding medication twice a day， for three consecutive weeks. During intragastric administration， the general conditions of rats in each group were observed， and the disease activity index （DAI） scores were assessed after the last administration. Serum levels of pro-inflammatory cytokines （tumor necrosis factor-α， interleukin-6） and anti-inflammatory cytokines （transforming growth factor-β1， interleukin-10） were measured. Pathological changes in their colonic tissues were observed and scored. Additionally， the content of SCFA （acetic acid， propionic acid and butyric acid） in their feces as well as the protein and mRNA expressions of GPR41， GPR43 and GPR109A in colonic tissues were detected. RESULTS Compared with the normal group， rats in the model group exhibited lethargy and obvious blood in their feces； the colonic tissue structure was severely damaged， with pathological changes such as notable glandular loss， edema， and inflammatory cell infiltration visible； the serum levels of pro- inflammatory cytokines， DAI score and colonic pathology score were significantly increased， while the levels of anti-inflammatory cytokines， SCFA content， and protein and mRNA expressions of GPR41， GPR43 and GPR109A were significantly decreased or down-regulated （P＜0.01）. Compared with the model group， the general condition and pathological changes of colonic tissue in each administration group showed improvement， with significant reversal observed in the aforementioned quantitative indicators （P＜0.05 or P＜0.01）. CONCLUSIONS Compound qinbai granules can alleviate intestinal inflammation and intestinal mucosal damage in UC rats. These effects may be related to its ability to restore intestinal SCFA levels and the expression of their target GPR. |
| 期刊： | 2025年第36卷第06期 |
| 作者： | 贺守炎；罗雯鹏；潘燎；肖金银；王真权 |
| AUTHORS： | HE Shouyan，LUO Wenpeng，PAN Liao，XIAO Jinyin，WANG Zhenquan |
| 关键字： | 复方芩柏颗粒；溃疡性结肠炎；短链脂肪酸；G蛋白偶联受体 |
| KEYWORDS： | Compound qinbai granules； ulcerative colitis； short-chain fatty acid； G protein-coupled receptor |
| 阅读数： | 4 次 |
| 本月下载数： | 0 次 |
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