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当归补血汤调控中性粒细胞胞外诱捕网改善卵巢早衰大鼠骨质疏松的机制研究
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| 篇名： | 当归补血汤调控中性粒细胞胞外诱捕网改善卵巢早衰大鼠骨质疏松的机制研究 |
| TITLE： | Study on the mechanism of Danggui buxue decoction regulating neutrophil extracellular traps to improve osteo-porosis in rats with premature ovarian failure |
| 摘要： | 目的 探讨当归补血汤调控中性粒细胞胞外诱捕网（NETs）改善卵巢早衰（POF）大鼠骨质疏松（OP）的机制。方法将雌性SD大鼠随机分成正常组、模型组、骨化三醇组及当归补血汤低、中、高剂量组，每组9只。除正常组外，其余各组大鼠于第1、8天以6mg/kg的剂量腹腔注射顺铂溶液建立POF并发OP模型。从造模第5天开始，各组大鼠灌胃相应药液或生理盐水，每天1次，连续4周。末次给药后，检测各组大鼠血清中雌二醇（E2）、NETs、25-羟维生素D3[25（OH）D3]、核因子κB受体激活蛋白配体（RANKL）、骨钙蛋白（BGP）水平，观察其骨组织病理学改变，并检测其骨组织中维生素D受体（VDR）、髓过氧化物酶（MPO）、中性粒细胞弹性蛋白酶（NE）、瓜氨酸化组蛋白H3（CitH3）、25羟基维生素D-1α-羟化酶（CYP27B1）、1α，25-二羟维生素D3-24-羟化酶（CYP24A1）蛋白的表达情况。结果与正常组相比，模型组大鼠骨组织骨小梁数量减少、变细及断裂且连接不完整，网状结构明显受损，骨髓腔增大；血清中NETs、RANKL水平和骨组织中MPO、NE、CitH3、CYP24A1蛋白的表达均显著升高或上调，血清中E2、25（OH）D3、BGP水平和骨组织中VDR、CYP27B1蛋白的表达均显著降低或下调（P＜0.05）。与模型组相比，各药物组大鼠骨组织病理学改变均有所恢复，多数定量指标显著改善（P＜0.05）。结论当归补血汤能够恢复POF并发OP大鼠的E2水平，改善OP，其机制可能与上调VD水平、抑制NETs形成有关。 |
| ABSTRACT： | OBJECTIVE To investigate the mechanism through which Danggui buxue decoction regulates neutrophil extracellular traps （NETs） to improve osteoporosis （OP） in rats with premature ovarian failure （POF）. METHODS Female SD rats were randomly divided into normal group， model group， calcitriol group， and Danggui buxue decoction low-dose， medium-dose and high-dose groups， with 9 rats in each group. Except for the normal group， all other groups were administered cisplatin via intraperitoneal injection on days 1 and 8 to establish a POF complicated with OP model. Each group received the corresponding drugs or normal saline intragastrically starting from day 5， once a day， for 4 consecutive weeks. After the last medication， serum levels of estradiol （E2）， NETs， 25-hydroxyvitamin D3 [25（OH）D3]， receptor activator of nuclear factor-κB ligand （RANKL）， and osteocalcin （BGP） were measured. The histopathological changes in bone tissue were observed. The expressions of vitamin D receptor （VDR）， myeloperoxidase （MPO）， neutrophil elastase （NE） and citrullinated histone H3 （CitH3） in bone tissue were detected； the protein expressions of 25-hydroxyvitamin D-1α-hydroxylase （CYP27B1） and 1α，25-dihydroxyvitamin D3-24-hydroxylase （CYP24A1） were also determined. RESULTS Compared with the normal group， the bone tissue of rats in the model group showed a significant reduction in the number of trabeculae， which was thinner broken and poorly connected， with significant destruction of the reticular structure， and an enlarged marrow cavity. Serum levels of NETs and RANKL， the protein expressions of MPO， NE， CitH3 and CYP24A1 in bone tissue were significantly increased or upregulated， while serum levels of E2， 25（OH）D3 and BGP as well as protein expressions of VDR and CYP27B1 were significantly decreased or downregulated （P＜0.05）. Compared with the model group， the histopathological changes in the bone tissue of rats in each administration group showed some degree of recovery， with significant improvements observed in most quantitative indicators （P＜0.05）. CONCLUSIONS Danggui buxue decoction can restore the E2 level in POF complicated with OP rats， and improve OP. The mechanism may be related to its ability to upregulate VD level and inhibit the formation of NETs. |
| 期刊： | 2025年第36卷第06期 |
| 作者： | 黄垂巧；陈帅；李倩；管连城；高洁；秦忠；陈云志 |
| AUTHORS： | HUANG Chuiqiao，CHEN Shuai，LI Qian，GUAN Liancheng，GAO Jie，QIN Zhong，CHEN Yunzhi |
| 关键字： | 当归补血汤；卵巢早衰；骨质疏松；中性粒细胞胞外诱捕网；维生素D |
| KEYWORDS： | Danggui buxue decoction； premature ovarian |
| 阅读数： | 4 次 |
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