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加味芍药甘草汤对慢传输型便秘大鼠肠道传输功能、肠道菌群及代谢产物含量的影响
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| 篇名： | 加味芍药甘草汤对慢传输型便秘大鼠肠道传输功能、肠道菌群及代谢产物含量的影响 |
| TITLE： | Effects of Modified shaoyao gancao decoction on intestinal transit function，intestinal flora and metabolite content in slow transit constipation rat |
| 摘要： | 目的 观察加味芍药甘草汤对慢传输型便秘（STC）大鼠肠道传输功能、肠道菌群及共代谢产物γ氨基丁酸（GABA）和5-羟色胺（5-HT）含量的影响。方法将SD大鼠随机分为空白组（10只）和造模组（30只），雌雄各半。造模组大鼠以复方地芬诺酯灌胃法构建STC模型。将造模成功的大鼠随机分为模型组、加味芍药甘草汤组[56g/（kg·d），以生药量计]、阳性对照药组[乳果糖2.09g/（kg·d）]，每组10只。各药物组大鼠灌胃相应药液，空白组和模型组大鼠灌胃等体积生理盐水，每天1次，连续14d。实验期间，观察各组大鼠的一般情况；末次给药后，称定其体重并对粪便性状进行Bristol评分，检测粪便含水率、肠道推进率和肠内容物中GABA、5-HT含量，考察肠内容物中肠道菌群的多样性，并分析GABA、5-HT含量与菌群相对丰度的关联性。结果与模型组比较，加味芍药甘草汤组大鼠体形瘦小、皮毛稀疏粗糙、行动迟缓等一般情况均有所改善，体重、Bristol评分、粪便含水率、肠道推进率、5-HT含量、Chao1和Shannon指数均显著升高，GABA含量、Simpson指数均显著降低（P＜0.05）。该组大鼠的肠道菌群可与空白组归为一类，但与模型组相距较远；该组脱硫杆菌门、厚壁菌门、拟杆菌门的相对丰度虽有回调，但与模型组比较差异均无统计学意义（P＞0.05）；脱硫杆菌门是组间差异因素（P＜0.05）。GABA含量与拟杆菌门、蓝菌门、髌骨菌门、放线菌门的相对丰度呈负相关（P＜0.01或P＜0.05），5-HT含量与弯曲杆菌门的相对丰度呈正相关（P＜0.05）。结论加味芍药甘草汤可改善STC模型大鼠粪便性状和肠道动力，上述作用可能与改善肠道菌群，进而影响肠内容物中GABA、5-HT含量有关；此外，GABA、5-HT含量可能与拟杆菌门、弯曲杆菌门等特定菌门的相对丰度显著相关。 |
| ABSTRACT： | OBJECTIVE To observe the effects of Modified shaoyao gancao decoction on intestinal transit function， intestinal flora and the contents of metabolites [γ aminobutyric acid （GABA） and 5-hydroxytryptamine （5-HT）] in slow transit constipation （STC） rats. METHODS SD rats were randomly divided into blank group （10 rats） and modeling group （30 rats）， with half male and half female. The STC model was established by intragastric administration of Compound diphenoxylate tablets in the modeling group. The successfully modeled rats were randomly divided into model group， Modified shaoyao gancao decoction group [56 g/（kg·d）， calculated by crude drug] and positive control group [lactulose 2.09 g/（kg·d）]， with 10 rats in each group. Each administration group was given relevant medicine intragastrically， the blank group and model group received an equivalent volume of normal saline， once a day， for 14 consecutive days. During the experiment， the general situation of rats was observed in each group. After the last medication， the body weight was measured， and the Bristol score was used to evaluate the fecal characteristics. The fecal moisture content， intestinal propulsion rate， and the contents of GABA and 5-HT in intestinal content were detected； the diversity of intestinal flora in intestinal contents was investigated， and the correlation between the contents of GABA， 5-HT and relative abundance of microbiota was analyzed. RESULTS Compared with the model group， general conditions such as small body shape， sparse and rough fur， and slow movement were all improved in Modified shaoyao gancao decoction body weight， Bristol score， fecal moisture content，intestinal propulsion rate， 5-HT content， Chao1 index and Shannon index were increased significantly， while GABA content and Simpson index were decreased significantly （P＜0.05）. The intestinal flora of rats in the Modified shaoyao gancao decoction group could be classified as the same as the blank group， but was far from the model group； the relative abundances of Desulfobacterota， Firmicutes and Bacteroidota in this group showed a tendency of pull back， but the differences were not statistically significant compared to model group （P＞0.05）. Desulfobacterota was an intergroup differential factor （P＜0.05）. The content of GABA was negatively correlated with the relative abundance of Bacteroidota， Cyanobacteria， Patescibacteria and Actinobacteriota （P＜0.05）. The content of 5-HT was positively correlated with the relative abundance of Campilobacterota （P＜0.05）. CONCLUSIONS Modified shaoyao gancao decoction can improve the fecal properties and intestinal motility of STC rats. Its mechanism may be related to improving intestinal flora and then affecting the contents of GABA and 5-HT in intestinal contents. In addition， the contents of GABA and 5-HT may be significantly correlated with the relative abundance of specific bacterial phyla such as Bacteroidota and Campilobacterota. |
| 期刊： | 2025年第36卷第02期 |
| 作者： | 张紫麒；周鸿雲；赵琼；邓媛；占煜 |
| AUTHORS： | ZHANG Ziqi，ZHOU Hongyun，ZHAO Qiong，DENG Yuan，ZHAN Yu |
| 关键字： | 加味芍药甘草汤；慢传输型便秘；肠道菌群；γ氨基丁酸；5-羟色胺 |
| KEYWORDS： | Modified shaoyao gancao decoction； slow transit constipation； intestinal flora； γ aminobutyric acid； 5- |
| 阅读数： | 4 次 |
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