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柿叶提取物对H2O2诱导IEC-6细胞铁死亡的影响及机制研究
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| 篇名： | 柿叶提取物对H2O2诱导IEC-6细胞铁死亡的影响及机制研究 |
| TITLE： | Effects and mechanism of persimmon leaf extract on IEC-6 cell ferroptosis induced by H2O2 |
| 摘要： | 目的 探讨柿叶（PL）提取物对过氧化氢（H2O2）诱导IEC-6细胞铁死亡的影响及潜在机制。方法以IEC-6细胞为对象，考察铁死亡抑制剂ferrostatin-1对H2O2诱导IEC-6细胞活力的影响；将IEC-6细胞分为对照组、H2O2组、H2O2+PL25μg/mL组、H2O2+PL50μg/mL组，检测其氧化应激指标[丙二醛（MDA）含量、超氧化物歧化酶（SOD）活性和活性氧（ROS）水平]、线粒体膜电位（MMP）以及核转录因子红系2相关因子2（Nrf2）、血红素氧合酶1（HO-1）、还原型烟酰胺腺嘌呤二核苷酸磷酸/醌氧化还原酶1（NQO-1）、胱氨酸/谷氨酸反向转运体（xCT）、谷胱甘肽过氧化物酶4（GPX4）、铁蛋白重链（FTH）mRNA及蛋白的表达情况。结果铁死亡抑制剂ferrostatin-1可显著升高H2O2诱导细胞的存活率（P＜0.01）。与对照组比较，H2O2组细胞MDA含量和ROS水平，Nrf2、NQO-1mRNA及Nrf2、HO-1蛋白的表达均显著升高或上调；SOD活性和MMP，xCT、GPX4、FTHmRNA及GPX4、FTH蛋白的表达均显著降低或下调（P＜0.01或P＜0.05）。与H2O2组比较，H2O2+PL25、50μg/mL组细胞的氧化应激指标均有不同程度回调，MMP均显著升高，Nrf2、HO-1、NQO-1，xCT、GPX4、FTHmRNA及蛋白的表达均有不同程度上调，部分指标组间比较差异有统计学意义（P＜0.01或P＜0.05）。结论PL提取物能减轻H2O2所导致的细胞线粒体膜损伤、ROS异常堆积，上述作用可能与激活Nrf2/HO-1信号通路从而抑制铁死亡有关。 |
| ABSTRACT： | OBJECTIVE To investigate the effects and potential mechanism of persimmon leaf （PL） extract against ferroptosis induced by H2O2 in IEC-6 cells. METHODS Using IEC-6 cells as object， the effects of ferroptosis inhibitor ferrostatin-1 on IEC-6 cell viability induced by H2O2 were investigated； IEC-6 cells were divided into control group， H2O2 group， H2O2+PL 25 μg/mL group and H2O2+PL 50 μg/mL group. The levels of oxidant stress indexes [content of malondialdehyde （MDA）， activity of superoxide dismutase （SOD）， and levels of reactive oxygen species （ROS）]， mitochondrial membrane potential （MMP） as well as mRNA and protein expressions of nuclear factor-erythroid-2 related factor 2 （Nrf2）， heme oxygenase-1 （HO-1）， NADPH/quinone oxidoreductase-1 （NQO-1）， cystine/glutamate anti-porter （xCT）， glutathione peroxidase 4 （GPX4） and ferritin heavy chain （FTH） were detected. RESULTS Ferroptosis inhibitor ferrostatin-1 could significantly increase the survival rate of H2O2-induced cells （P＜ 0.01）. Compared with the control group， MDA content， ROS level， mRNA expressions of Nrf2 and NQO-1 as well as protein expressions of Nrf2 and HO-1 were increased or up-regulated significantly， while SOD activity， MMP， mRNA expressions of xCT， GPX4 and FTH as well as protein expressions of GPX4 and FTH were decreased or down-regulated significantly （P＜0.01 or P＜0.05）. Compared with the H2O2 group， oxidative stress Δ indexes of H2O2+PL 25， 50 μg/mL groups were reversed to different extents， MMP level was increased significantly， as well as mRNA and protein expressions of Nrf2， HO-1， NQO-1，xCT， GPX4 and FTH were up-regulated to different extents；there were statistical significances in some indexes between groups （P＜0.01 or P＜0.05）. CONCLUSIONS PL extract can alleviate mitochondrial membrane damage and abnormal accumulation of ROS caused by H2O2， which may be related to the inhibition of ferroptosis by activating the Nrf2/HO-1 signaling pathway. |
| 期刊： | 2025年第36卷第01期 |
| 作者： | 张雪侠；周敏；周红艳；王莉飞；李华妮；刘长河；徐洪德；张明利 |
| AUTHORS： | ZHANG Xuexia，ZHOU Min，ZHOU Hongyan，WANG Lifei，LI Huani，LIU Changhe，XU Hongde，ZHANG Mingli |
| 关键字： | 柿叶提取物；溃疡性结肠炎；铁死亡；Nrf2/HO-1信号通路 |
| KEYWORDS： | persimmon leaf extract； ulcerative colitis； ferroptosis； Nrf2/HO-1 signaling pathway |
| 阅读数： | 9 次 |
| 本月下载数： | 0 次 |
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