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灯红补阳还五颗粒指纹图谱及有效成分含量研究
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| 篇名： | 灯红补阳还五颗粒指纹图谱及有效成分含量研究 |
| TITLE： | Study on the fingerprint and active ingredients contents of Denghong buyang huanwu granules |
| 摘要： | 目的 研究灯红补阳还五颗粒（DBHG）的指纹图谱，筛选其质量标志物，并建立测定有效成分含量的方法。方法采用高效液相色谱（HPLC）法。使用《中药色谱指纹图谱相似度评价系统（2012版）》建立10批（S1～S10）DBHG的指纹图谱，评价其相似度，并对共有峰进行药味归属分析、聚类分析（CA）、主成分分析（PCA）以及正交偏最小二乘法-判别分析（OPLS-DA），以变量重要性投影（VIP）值＞1为标准筛选质量标志物；采用同一HPLC法测定10批样品中红景天苷、酪醇、毛蕊异黄酮葡萄糖苷、野黄芩苷、毛蕊异黄酮的含量。结果从10批样品的指纹图谱中，共获得25个共有峰，指认了6个共有峰，分别为红景天苷、酪醇、芍药苷、毛蕊异黄酮葡萄糖苷、野黄芩苷、毛蕊异黄酮；10批样品相似度均大于0.9，其中10个共有峰为灯盏细辛特有成分。CA、PCA均可将DBHG聚为2类，S4～S5、S7样品为一类，其余样品为一类。16（野黄芩苷）、12、15（毛蕊异黄酮葡萄糖苷）、13（芍药苷）、14号峰对应的成分为DBHG的质量标志物。10批样品中红景天苷、酪醇、毛蕊异黄酮葡萄糖苷、野黄芩苷、毛蕊异黄酮的平均含量分别为1.64、0.45、0.31、0.73、0.15mg/g。结论成功建立了DBHG的HPLC指纹图谱和红景天苷等5个有效成分的含量测定方法，筛选得到5个质量标志物，可用于该制剂的质量控制。 |
| ABSTRACT： | OBJECTIVE To study the fingerprint of Denghong buyang huanwu granules （DBHG）， screen the quality markers， and establish the method for content determination of active ingredients. METHODS HPLC method was adopted. The fingerprints of 10 batches of DBHG （S1-S10） were established by using the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine （2012 edition）， and similarity evaluation was also performed. Traditional Chinese medicine pieces attribution analysis， cluster analysis （CA）， principal component analysis （PCA） and orthogonal partial least squares discriminant analysis （OPLS-DA） were conducted for common peaks， and quality biomarkers were screened based on variable importance projection （VIP） values＞1. The contents of 10 batches of samples were determined by the same HPLC method， such as salidroside， tyrosol， calycosin-7-O-beta-D-glucoside， scutellarin and calycosin. RESULTS A total of 25 common peaks were obtained in the fingerprints for 10 batches of samples and 6 common peaks were identified， i.e. salidroside， tyrosol， paeoniflorin， calycosin-7-O-beta-D-glucoside， scutellarin， calycosin. Their similarities were greater than 0.9， and 10 common peaks of them were unique components of Erigeron breviscapus. DBHG could be clustered into 2 categories by using CA and PCA； S4-S5， and S7 could be clustered into one category and other samples could be clustered into one category. The corresponding components of peaks 16 （scutellarin）， 12， 15 （calycosin-7-O-beta-D-glucoside）， 13 （paeoniflorin）， and 14 were quality markers. The average contents of salidroside， tyrosol， calycosin-7-O-beta-D-glucoside， scutellarin and calycosin were 1.64， 0.45， 0.31， 0.73， 0.15 mg/g in 10 batches of samples. CONCLUSIONS HPLC fingerprint for DBHG and a method for determining the contents of five active ingredients including salidroside are successfully established. Five quality markers have been screened. It can be used for the quality control of the preparation. |
| 期刊： | 2024年第35卷第20期 |
| 作者： | 药雅俊；甄会贤；王嘉琛；赵雅清；张虹 |
| AUTHORS： | YAO Yajun，ZHEN Huixian，WANG Jiachen，ZHAO Yaqing，ZHANG Hong |
| 关键字： | 灯红补阳还五颗粒；质量标志物；指纹图谱；多成分定量；化学计量学分析 |
| KEYWORDS： | Denghong buyang huanwu granules； quality marker； fingerprint； multi-component quantification； chemo-metrics |
| 阅读数： | 3 次 |
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