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鳖龙软肝汤对肝癌模型大鼠的影响及作用机制
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| 篇名： | 鳖龙软肝汤对肝癌模型大鼠的影响及作用机制 |
| TITLE： | Effects and mechanism of Bielong ruangan decoction on liver cancer model rats |
| 摘要： | 目的 探讨鳖龙软肝汤对肝癌模型大鼠的影响及作用机制。方法将32只雄性SD大鼠随机分为对照组、模型组和鳖龙软肝汤低、高剂量组[6.84、27.36g/（kg·d），以生药量计]，每组8只。除对照组外，其余各组大鼠均腹腔注射二乙基亚硝胺（DEN）50mg/kg（每周1次，连续16周）以复制肝癌模型。于DEN注射第8周时，鳖龙软肝汤低、高剂量组大鼠灌胃相应药液，每天2次，给药至第16周。观察各组大鼠实验期间的一般状况。末次给药后，称定其体重和肝脏质量，计算肝指数；检测其血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）含量；观察其肝脏外观、病理形态和纤维化程度，并进行肝纤维化Ishak评分；检测肝组织中核苷酸结合结构域富含亮氨酸重复序列和含热蛋白结构域受体3（NLRP3）、胱天蛋白酶1（caspase-1）、消皮素D（GSDMD）、白细胞介素18（IL-18）、IL-1βmRNA及蛋白的表达情况。结果与模型组比较，鳖龙软肝汤低、高剂量组大鼠一般状态（低剂量组除外）和肝脏质地、表面结节和肿瘤块、炎症细胞浸润及异形细胞数量均有所改善；肝指数（低剂量组除外），Ishak评分（低剂量组除外），血清中ALT、AST含量，以及肝组织中NLRP3、caspase-1、GSDMD、IL-18、IL-1βmRNA和蛋白的表达量均显著降低（P＜0.05），其中高剂量组上述部分指标显著低于低剂量组（P＜0.05）。结论鳖龙软肝汤可抑制大鼠肝癌进程，减轻肝损伤，其作用机制可能与抑制NLRP3/caspase-1/GSDMD信号通路、改善炎症反应有关。 |
| ABSTRACT： | OBJECTIVE To explore the effects and mechanism of Bielong ruangan decoction on liver cancer model rats. METHODS Thirty-two male SD rats were randomly divided into control group， model group， and Bielong ruangan decoction low- dose and high-dose groups [6.84， 27.36 g/（kg·d）， by raw material]， with 8 rats in each group. Except for the control group， other groups were intraperitoneally injected with diethylnitrosamine （DEN） 50 mg/kg （once a week， for 16 consecutive weeks） to induce liver cancer model. At the 8th week of DEN injection， Bielong ruangan decoction low-dose and high-dose groups were orally administered with the corresponding medication， twice a day， until the 16th week. The general condition of rats in each group was observed during the experimental period. After the final administration， the body weight and liver mass were weighed， and the liver indexes were calculated； serum contents of alanine transaminase （ALT） and aspartate transaminase （AST） were determined； the appearance， pathological morphology and degree of fibrosis of liver were observed； Ishak scoring for liver fibrosis was performed； the mRNA and protein expressions of nucleotide-binding domain leucine-rich repeat and pyrin domain-containing receptor 3 （NLRP3）， caspase-1， gasdermin D （GSDMD）， interleukin-18 （IL-18）， IL-1β in liver tissue were detected. RESULTS Compared with the model group， the general condition of rats （except for the low-dose group）， liver texture， surface nodules and tumors， inflammatory cell infiltration and abnormal cell amount were all improved in Bielong ruangan decoction low-dose and high-dose groups. Liver index （except for low-dose group）， Ishak score （except for low-dose group）， the serum contents of ALT and AST， as well as the mRNA and protein expressions of NLRP3， caspase-1， GSDMD， IL-18 and IL-1β in liver tissue were reduced significantly （P＜0.05）， with some of above indicators in high-dose group being significantly lower than those in low-dose group （P＜0.05）. CONCLUSIONS Bielong ruangan decoction can inhibit the progression of liver cancer in rats and reduce liver damage. Its mechanism of action may be related to the inhibition of the NLRP3/caspase-1/GSDMD signaling pathway and the improvement of inflammatory response. |
| 期刊： | 2024年第35卷第18期 |
| 作者： | 银思涵；彭憬；罗凌威；周意；胡莉 |
| AUTHORS： | YIN Sihan，PENG Jing，LUO Lingwei，ZHOU Yi，HU Li |
| 关键字： | 鳖龙软肝汤；肝癌；NLRP3/caspase-1/GSDMD信号通路；炎症反应 |
| KEYWORDS： | Bielong ruangan decoction； liver cancer； NLRP3/caspase-1/GSDMD signaling pathway； inflammatory response |
| 阅读数： | 4 次 |
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