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术后通气方对术后肠麻痹小鼠肠道损伤的改善作用及机制
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| 篇名： | 术后通气方对术后肠麻痹小鼠肠道损伤的改善作用及机制 |
| TITLE： | Improvement effect and mechanism of Shuhou tongqi formula on intestinal injury in mice with postoperative ileus |
| 摘要： | 目的 研究术后通气方对术后肠麻痹（POI）小鼠肠道损伤的改善作用及机制。方法将小鼠随机分为假手术组、模型组、阳性对照组（枸橼酸莫沙必利片，1.95mg/kg）、术后通气方组（1.88g/kg），每组6只。除假手术组外，其余各组小鼠使用经典小肠干扰术制备POI模型。各组小鼠灌胃相应药液/水，每天1次，连续2d。末次给药后，检测小鼠肠道炭末推进率；观察小鼠回肠组织病理形态学变化；检测小鼠血清中白细胞介素6（IL-6）、IL-10、肿瘤坏死因子α（TNF-α）、胃动素（MTL）和生长抑素（SS）水平；检测小鼠回肠组织中Toll样受体4（TLR4）、核因子κBp65（NF-κBp65）、p38丝裂原活化蛋白激酶（p38MAPK）表达水平；分析小鼠结肠内容物的肠道菌群结构。结果经术后通气方干预后，小鼠回肠组织肠壁萎缩、黏膜毛细血管充血等病理损伤明显改善；小肠炭末推进率和血清中IL-10、MTL水平均显著升高（P＜0.05）；血清中TNF-α、IL-6、SS水平，回肠组织中TLR4、NF-κBp65、p38MAPK蛋白核内表达水平均显著降低（P＜0.05）。肠道菌群分析结果显示，术后通气方可显著升高ACE、Chao1、Shannon、PD指数以及阿克曼氏菌属（Akkermansia）相对丰度（P＜0.05），显著降低变形菌门（Proteobacteria）、酸鱼联合乳杆菌属（Ligilactobacillus）、大肠埃希菌属-志贺氏菌属（Escherichia-Shigella）相对丰度（P＜0.05）。结论术后通气方可改善POI小鼠肠道损伤及炎症反应，其作用机制可能与抑制TLR4/NF-κB/MAPK信号通路活性，调节胃肠激素水平，改善肠道菌群紊乱有关。 |
| ABSTRACT： | OBJECTIVE To study the improvement effect and mechanism of Shuhou tongqi formula on intestinal injury in mice with postoperative ileus （POI）. METHODS Mice were randomly divided into sham operation group， model group， positive control group （Mosapride citrate tablets， 1.95 mg/kg）， and Shuhou tongqi formula group （1.88 g/kg）， with 6 mice in each group. Except for the sham operation group， POI model was induced in other groups by typical small intestinal interference. Each group was given relevant drug liquid/water， once a day， for consecutive 2 days. After the last medication， the percentage of carbon powder propulsion in small intestine was detected， and pathomorphological changes in ileum tissue of mice were observed. The serum levels of interleukin-6 （IL-6）， IL-10， tumor necrosis factor-α （TNF-α）， motilin （MTL） and somatostatin （SS） were all detected； the expression levels of Toll-like receptor 4 （TLR4）， nuclear factor κB （NF-κB p65） and p38 mitogen-activated protein kinase （p38 MAPK） were determined in ileal tissue of mice； the gut microbiota of colon contents was analyzed in each group of mice. RESULTS After the intervention of Shuhou tongqi formula， pathological damage such as intestinal wall atrophy and mucosal capillary congestion in ileum tissue were improved significantly； the percentage of carbon powder propulsion and the serum level of IL-10 and MTL were increased significantly （P＜0.05）； however， serum levels of TNF-α， IL-6 and SS， the expressions of TLR4， NF-κB p65 and p38 MAPK were decreased significantly （P＜0.05）. The analysis of gut microbiota showed that Shuhou tongqi formula could significantly increase ACE， Chao1， Shannon and PD indexes， and relative abundance of Akkermansia （P＜0.05）， but decreased relative abundance of Proteobacteria， Ligilactobacillus and Escherichia-Shigella significantly （P＜0.05）. CONCLUSIONS Shuhou tongqi formula can improve intestinal injury and inflammatory reaction of POI mice， the mechanism of which may be associated with inhibiting the activity of TLR4/ NF-κB/MAPK signaling pathway， regulating the levels of gastrointestinal hormones and improving the disturbance of intestinal flora in mice. E-mail：1643589936@qq.com |
| 期刊： | 2024年第35卷第17期 |
| 作者： | 卢雨暄；江志伟；谢怡君；王弈竹；哈静文；束雅春 |
| AUTHORS： | LU Yuxuan，JIANG Zhiwei，XIE Yijun，WANG Yizhu，HA Jingwen，SHU Yachun |
| 关键字： | 术后通气方；术后肠麻痹；肠道损伤；炎症因子；肠道菌群 |
| KEYWORDS： | Shuhou tongqi formula； postoperative ileus； |
| 阅读数： | 5 次 |
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