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巴戟天水提液对双酚A染毒小鼠性激素及受体表达的影响
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| 篇名： | 巴戟天水提液对双酚A染毒小鼠性激素及受体表达的影响 |
| TITLE： | Effects of the water extract of Morinda officinalis on the expressions of sex hormones and receptors in bisphenol A-contaminated mice |
| 摘要： | 目的 探讨巴戟天水提液对双酚A（BPA）染毒小鼠性激素及受体表达的影响。方法将60只雄性昆明小鼠随机分为对照组、模型组和巴戟天水提液低、中、高剂量组（20、40、60mg/kg），每组12只。模型组和巴戟天水提液各剂量组小鼠灌胃BPA[50mg/（kg·d），每天1次，连续4周]以建立BPA染毒模型。造模后，各药物组小鼠灌胃相应药液，每天1次，连续4周。末次给药后，称定各组小鼠的体重和睾丸质量，观察其睾丸组织病理改变，检测其血清性激素[黄体生成素（LH）、卵泡刺激素（FSH）]含量和睾丸组织中LH受体（LHR）、FSH受体（FSHR）mRNA及蛋白的表达情况。结果与对照组比较，模型组小鼠睾丸组织存在结构变性，生精细胞与支持细胞之间连接松散、出现明显裂隙，且生精细胞数量减少；睾丸组织中LHR、FSHRmRNA及蛋白的表达均显著下调（P＜0.01），但其体重、睾丸质量和血清LH、FSH含量均无明显变化（P＞0.05）。与模型组比较，巴戟天水提液各剂量组小鼠睾丸组织病理改变均有不同程度改善；睾丸组织中LHR、FSHRmRNA及蛋白的表达亦有不同程度上调（P＜0.05或P＜0.01），且部分指标水平与对照组相当（P＞0.05），但其体重、睾丸质量和血清LH、FSH含量无明显变化（P＞0.05）。结论巴戟天水提液对BPA染毒小鼠睾丸损伤有一定的改善作用，可能与其提高LHR、FSHR蛋白及mRNA的表达有关。 |
| ABSTRACT： | OBJECTIVE To explore the effects of the water extract of Morinda officinalis on the expressions of sex hormones and receptors in bisphenol A （BPA）-contaminated mice. METHODS Totally 60 male Kunming mice were randomly divided into control group， model group， and low-dose， medium-dose and high-dose groups of M. officinalis water extract （20， 40， 60 mg/kg）， with 12 mice in each group. The model group and M. officinalis water extract groups were given BPA intragastrically [50 mg/（kg·d）， once a day， for 4 consecutive weeks] to establish the BPA-contamination model of mice. After modeling， each drug group was gavaged with the corresponding drug solution， once a day， for 4 consecutive weeks. After the last medication， the body weight and testicular weight of the mice in each group were weighed， the histopathological changes in the testis were observed， and the serum sex hormones [luteinizing hormone （LH）， follicle-stimulating hormone （FSH）] contents and the mRNA and protein expressions of LH receptor （LHR） and FSH receptor （FSHR） in the testicular tissues were detected. RESULTS Compared with the control group， the testicular tissues of mice in the model group had structural degeneration， loose connections between spermatogenic cells and Sertoli cells， obvious lacunae and reduced number of spermatogenic cells； the mRNA and protein expressions of LHR and FSHR in testicular tissues were significantly down-regulated （P＜0.01）， but there were no significant changes in their body weights， testicular weights， and serum contents of LH and FSH （P＞0.05）. Compared with the model group， the histopathological changes of testicular tissues of mice in each dose group of M. officinalis water extract were improved to different degrees， and the mRNA and protein expressions of LHR and FSHR in testicular tissues were up-regulated to different degrees （P＜0.05 or P＜ 0.01）， and some indicator levels were similar to those of the control group （P＞0.05）. However， there were no significant changes in their body weights， testicular weights， and serum contents of LH and FSH （P＞0.05）. CONCLUSIONS The water extract of M. officinalis has a certain improvement effect on testicular injury in BPA-contaminated mice， which might be related to its increase in the mRNA and protein expressions of LHR and FSHR. |
| 期刊： | 2024年第35卷第16期 |
| 作者： | 张利；信小兵；胡欢欢；李晓林；董红晓；蒋香菊 |
| AUTHORS： | ZHANG Li，XIN Xiaobing，HU Huanhuan，LI Xiaolin，DONG Hongxiao，JIANG Xiangju |
| 关键字： | 巴戟天；水提液；双酚A；性激素；性激素受体；睾丸 |
| KEYWORDS： | Morinda officinalis； water extract； bisphenol A； |
| 阅读数： | 3 次 |
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