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含羞草根乙酸乙酯部位对急性髓系白血病小鼠的影响
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| 篇名： | 含羞草根乙酸乙酯部位对急性髓系白血病小鼠的影响 |
| TITLE： | Effect of ethyl acetate extract from Mimosa pudica root on acute myeloid leukemia in mice |
| 摘要： | 目的 研究含羞草根乙酸乙酯部位（简称为乙酸乙酯部位）对急性髓系白血病小鼠的影响。方法以不同质量浓度（0.0625、0.125、0.25、0.5mg/mL）乙酸乙酯部位作用于急性粒-单核细胞白血病细胞WEHI-3，考察其对该细胞活力的影响。将50只BALB/C小鼠随机分为空白对照组、模型组、阳性对照组（5-氟尿嘧啶，13mg/kg）和乙酸乙酯部位低、高剂量组（50、200mg/kg），每组10只。除空白对照组外，其余各组小鼠均腹腔注射WEHI-3细胞悬液进行造模，并在造模第2天起灌胃相应药物/水，每天1次，连续14d。末次给药后，检测小鼠肝、脾指数，并进行肝组织病理学形态观察和血液学分析以及白细胞分化检测；检测小鼠血清中细胞因子[白细胞介素2（IL-2）、IL-3、干扰素γ（IFN-γ）、肿瘤坏死因子α（TNF-α）]水平；检测小鼠全血中白细胞表面标志物[分化簇3（CD3）、CD19、CD11b、分化簇107b（即Mac-3）]抗体水平。结果经0.0625～0.5mg/mL的乙酸乙酯部位作用后，WEHI-3细胞增殖抑制率均显著升高（P＜0.05）。经高剂量的乙酸乙酯部位干预后，小鼠的肝、脾指数，血清中TNF-α水平，全血中白细胞表面CD11b、Mac-3抗体水平均显著降低（P＜0.05），血清中IL-2、IL-3、IFN-γ水平和全血中白细胞表面CD3、CD19抗体水平均显著升高（P＜0.05）；肝实质偶有淋巴细胞浸润，几乎无炎症细胞浸润；血液学指标改善且白细胞分化减弱。结论含羞草根乙酸乙酯部位可抑制WEHI-3细胞增殖，改善急性髓系白血病小鼠症状，其作用机制与增强机体免疫功能有关。 |
| ABSTRACT： | OBJECTIVE To study the inhibitory effect of ethyl acetate extract from Mimosa pudica root （ethyl acetate extract for short） on acute myeloid leukemia in mice. METHODS Different concentrations of ethyl acetate extract （0.062 5， 0.125， 0.25， 0.5 mg/mL） were used to treat acute myelomonocytic leukemia cell lines WEHI-3， and their effects on cell viability were investigated. Fifty BALB/C mice were randomly divided into blank control group， model group， positive control group （5- fluorouracil， 13 mg/kg）， and ethyl acetate extract low-dose and high-dose groups （50， 200 mg/kg）， with 10 mice in each group. Except for the blank control group， the leukemia model was constructed by intraperitoneal injection of WEHI-3 cells in other groups， and from the second day of modeling， corresponding drugs/water were orally administered once a day for 14 consecutive days. After the last administration， the liver and spleen indexes of mice were measured， and liver tissue pathological morphology observation， hematological analysis， and white blood cell differentiation detection were performed； the levels of cytokine [interleukin-2 （IL-2）， IL-3， interferon-γ （IFN-γ）， tumor necrosis factor-α （TNF-α）] in serum were determined； the levels of leukocyte surface markers [cluster of differentiation 3 （CD3）， CD19， CD11b， CD107b （Mac-3）] in whole blood were all detected. RESULTS After treated with 0.062 5-0.5 mg/mL ethyl acetate， the inhibition rate of cell proliferation were increased significantly （P＜0.05）. After intervention with high-dose ethyl acetate， the liver and spleen index， serum level of TNF-α， the levels of CD11b and Mac-3 in blood were significantly reduced （P＜0.05）， while serum levels of IL-2， IL-3 and IFN-γ， and the levels of CD3 and CD19 in blood were increased significantly （P＜0.05）. Occasional lymphocyte infiltration was present in the liver parenchyma， with almost no infiltration of inflammatory cells； hematology improvement and weakened white blood cell differentiation were found. CONCLUSIONS The ethyl acetate extract of M. pudica root can inhibit the proliferation of WEHI-cells， and improve symptoms in acute myeloid leukemia mice， the mechanism of which may be associated with enhancing the immune function. |
| 期刊： | 2024年第35卷第11期 |
| 作者： | 邓婉柔；李慧敏；曾元宁；王秋红 |
| AUTHORS： | DENG Wanrou，LI Huimin，ZENG Yuanning，WANG Qiuhong |
| 关键字： | 含羞草根；乙酸乙酯部位；急性髓系白血病；白细胞分化；免疫功能 |
| KEYWORDS： | Mimosa pudica root； ethyl acetate extract； acute myeloid leukemia； white blood cell differentiation； immune |
| 阅读数： | 1 次 |
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