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杜仲改善大鼠产后抑郁的作用机制研究
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| 篇名： | 杜仲改善大鼠产后抑郁的作用机制研究 |
| TITLE： | Regulatory effect mechanism of Eucommia ulmoides on postpartum depression in rats |
| 摘要： | 目的 探讨杜仲改善大鼠产后抑郁的作用机制。方法受孕雌鼠随机分为正常组、产后抑郁组和杜仲低、高剂量组（1.34、2.68g/kg，以生药计），每组10只。除正常组外其余各组大鼠运用孕期旁观电击法建立产后抑郁大鼠模型；造模的同时，给药组大鼠灌胃相应药物，正常组及产后抑郁组大鼠灌胃生理盐水，持续21d。观察各组大鼠实验期间的一般情况，通过旷场实验、Morris水迷宫实验和糖水偏好实验进行行为学评价，检测各组大鼠血清中皮质酮（CORT）、下丘脑中促肾上腺皮质激素释放因子（CRF）和尿皮质素（UCN）、垂体中促肾上腺皮质激素（ACTH）水平，以及海马组织中CRF受体1（CRFR1）、CRFR2及电压依赖性阴离子通道1（VDAC1）蛋白表达水平，海马组织凋亡细胞比例及JC-1高电位细胞比例，并观察海马组织形态。结果与产后抑郁组比较，杜仲高剂量组大鼠食欲、精神、毛色均有所改善，体重有所增加；垂直运动、水平运动、自我梳理得分均显著升高（P＜0.05）；第2～4天逃避潜伏期均显著缩短；穿越平台次数显著增加，每次穿越时间显著延长（P＜0.05）；孕20d及产后30d糖水消耗比率显著升高（P＜0.05）；CRF、UCN、ACTH、CORT水平，噬仔率，CRFR2、VDAC1蛋白表达水平，海马组织凋亡细胞比例均显著降低（P＜0.05）；JC-1高电位细胞比例显著升高（P＜0.05）；神经元细胞周围水肿现象明显改善。结论杜仲可能通过抑制下丘脑-垂体-肾上腺轴的过度激活，降低CRFR2的表达水平，进而抑制VDAC1的表达，并减少神经元细胞的凋亡，从而改善产后抑郁症状。 |
| ABSTRACT： | OBJECTIVE To explore the effect mechanism of Eucommia ulmoides on improving postpartum depression in rats. METHODS Pregnant rats were randomly divided into normal group， postpartum depression group， and low-dose and high-dose groups of E. ulmoides （1.34， 2.68 g/kg， calculated by crude drug）， with 10 rats in each group. Except for the normal group， the rats in other groups suffered from fear stress to induce postpartum depression model during pregnancy； at the same time of modeling， the administration groups were given relevant medicine intragastrically， while the normal group and postpartum depression group were given physiological saline intragastrically for 21 days. Postpartum behaviors of rats during the experiment were assessed using the open field test， Morris water maze test and sucrose preference test. Additionally， the levels of corticosterone （CORT） in serum， corticotropin releasing factor （CRF） and urocortin （UCN） in hypothalamus， and adrenocorticotropic hormone （ACTH） in hypophysis were detected； meanwhile， the protein expressions of CRF receptor 1 （CRFR1）， CRFR2， and voltage-dependent anion channel 1 （VDAC1） in hippocampal tissue were measured； the proportions of apoptotic cells and JC-1 high potential cells in hippocampal tissue were determined， and the morphology of hippocampal tissue was observed. RESULTS Compared with postpartum depression group， the high-dose group of E. ulmoides showed improvements in appetite， mental state， and hair color in rats； their body weight had increased； the scores of vertical movement， horizontal movement and self-sorting significantly increased； from the 2ed to 4th day avoidance latency significantly shortened， and the times of crossing the platform and the time of crossing the platform Δ 基金项目国家自然科学基金青年基金项目（No.82204789） significantly increased/prolonged （P＜0.05）； the ratio of glucose and water consumption significantly increased at 20 days of pregnancy and 30 days postpartum （P＜0.05）； the levels of CRF， UCN， ACTH and CORT， phagocytic rate， protein expressions of CRFR2 and VDAC1， and the proportion of apoptosis cells in hippocampal tissue were decreased significantly （P＜0.05）； the proportion of JC-1 high potential cells significantly increased （P＜0.05）， and the phenomenon of edema around neuronal cells was significantly improved. CONCLUSIONS E. ulmoides can improve postpartum depression by inhibiting excessive activation of hypothalamic-pituitary-adrenal axis， decreasing the expression of CRFR2， thereby inhibiting the expression of VDAC1， and decreasing the apoptosis of neuronal cells. |
| 期刊： | 2024年第35卷第11期 |
| 作者： | 李玉洁；陈文静；张岚；吴英真；许永杰；王小兰；侯俊林；杨丽萍 |
| AUTHORS： | LI Yujie，CHEN Wenjing，ZHANG Lan，WU Yingzhen，XU Yongjie，WANG Xiaolan，HOU Junlin，YANG Liping |
| 关键字： | 杜仲；产后抑郁；下丘脑-垂体-肾上腺轴；促肾上腺皮质激素释放因子受体；电压依赖性阴离子通道1；神经元细胞 |
| KEYWORDS： | Eucommia ulmoides； postpartum depression； HPA axis； corticotropin releasing factor receptor； voltage-dependent |
| 阅读数： | 2 次 |
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