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基于定性、定量方法的浮小麦与小麦差异成分分析
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| 篇名： | 基于定性、定量方法的浮小麦与小麦差异成分分析 |
| TITLE： | Differential component analysis between Fructus Tritici Levis and Triticum aestivum based on qualitative and quantitative methods |
| 摘要： | 目的 分析浮小麦和小麦的成分差异，为二者的鉴定及质量控制提供参考。方法收集20批浮小麦和3批小麦药材，采用高效液相色谱法，利用《中药色谱指纹图谱相似度评价系统》（2012版）绘制指纹图谱并进行相似度评价，应用聚类分析（CA）、主成分分析（PCA）和正交偏最小二乘判别分析（OPLS-DA）对不同产地浮小麦及小麦样品进行差异分析，筛选差异成分，并测定浮小麦和小麦中6个指认成分的含量。结果浮小麦指纹图谱的相似度为0.928～0.996，小麦与浮小麦的相似度为0.761～0.773；在浮小麦和小麦中共标定19个共有峰，指认出了6个成分，分别为亚麻酸、亚油酸、5-十七烷基间苯二酚、5-十九烷基间苯二酚、5-二十一烷基间苯二酚和5-二十三烷基间苯二酚。CA、PCA结果显示，浮小麦和小麦能明显区分；产地为安徽的浮小麦的分布较为集中。OPLS-DA结果显示，亚麻酸、亚油酸及其他6个未知化合物为浮小麦和小麦的差异成分。浮小麦中上述6个指认成分的平均含量分别为0.1009、1.0940、0.0051、0.0309、0.0982、0.0248mg/g，且浮小麦中亚麻酸、亚油酸的含量均显著高于小麦（P＜0.05）。结论所建定性、定量测定方法操作简单、结果可靠，可用于浮小麦和小麦的鉴别及质量评价。亚麻酸、亚油酸等差异成分可为浮小麦和小麦的鉴别及药效学差异研究提供线索。 |
| ABSTRACT： | OBJECTIVE To analyze the compositional differences between Fructus Tritici Levis and Triticum aestivum， and to provide reference for identification and quality control of both. METHODS Twenty batches of Fructus Tritici Levis and three batches of T. aestivum were collected， and their fingerprints were acquired by high-performance liquid chromatography and the similarities were evaluated by the Evaluation System of Similarity of Chromatographic Fingerprints of Traditional Chinese Medicine （2012 version）. Cluster analysis （CA）， principal component analysis （PCA） and orthogonal partial least squares-discriminant analysis （OPLS-DA） were performed to analyze the difference of Fructus Tritici Levis and T. aestivum from different regions， and the differential components were screened. The contents of the six identified components in Fructus Tritici Levis and T. aestivum were determined. RESULTS The similarities of the fingerprints of Fructus Tritici Levis ranged from 0.928 to 0.996， and the relative similarities of T. aestivum with Fructus Tritici Levis ranged from 0.761 to 0.773. A total of 19 common peaks were calibrated， and six components including linolenic acid， linoleic acid， 5-heptadecylresorcinol， 5-nonadodecylresorcinol， 5- heneicosylresorcinol， and 5-tricosylresorcinol were identified. The results of CA and PCA showed that Fructus Tritici Levis and T. aestivum could be clearly distinguished； the distribution of Fructus Tritici Levis from Anhui province was relatively concentrated. The results of OPLS-DA showed that linolenic acid， linoleic acid， and other six unknown compounds were the differential components between Fructus Tritici Levis and T. aestivum. The average contents of the six identified components in Fructus Tritici Levis were 0.100 9， 1.094 0， 0.005 1， 0.030 9， 0.098 2，and 0.024 8 mg/g， respectively； the contents of linolenic acid and linoleic acid in Fructus Tritici Levis were significantly higher than those in T. aestivum （P＜0.05）.CONCLUSIONS The established qualitative and quantitative methods are simple and reliable， and can be used for the identification and quality evaluation of Fructus Tritici Levis and T. aestivum. The identified differential components， such as linolenic acid and linoleic acid， can also provide clues for the differentiation and pharmacological study of Fructus Tritici Levis and T. aestivum. |
| 期刊： | 2024年第35卷第11期 |
| 作者： | 李雪娇；胡雨；陈赟；商娟；李臻阳；封云铧；邹建东；姚卫峰；卢苏；许美娟 |
| AUTHORS： | LI Xuejiao，HU Yu， CHEN Yun，SHANG Juan，LI Zhenyang，FENG Yunhua，ZOU Jiandong，YAO Weifeng，LU Su，XU Meijuan |
| 关键字： | 浮小麦；小麦；指纹图谱；含量测定；化学计量学分析；差异成分；高效液相色谱法 |
| KEYWORDS： | Fructus Tritici Levis； Triticum aestivum； fingerprint； content determination； chemometrics analysis； differential |
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