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鼠曲草抗慢性阻塞性肺疾病的质量标志物筛选
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| 篇名： | 鼠曲草抗慢性阻塞性肺疾病的质量标志物筛选 |
| TITLE： | Screening of anti-COPD quality biomarkers of Gnaphalium affine |
| 摘要： | 目的 筛选鼠曲草抗慢性阻塞性肺疾病（COPD）的质量标志物并测定其含量。方法通过网络药理学以“鼠曲草”为检索条件预测其抗COPD的有效成分及靶点。采用《中药色谱指纹图谱相似度评价系统（2012年版）》建立10批鼠曲草药材（S1～S10）的高效液相色谱（HPLC）指纹图谱，指认共有峰，进行相似度评价；并进行聚类分析、主成分分析和正交偏最小二乘-判别分析，以变量重要性投影（VIP）＞1为标准筛选影响鼠曲草质量的差异性成分作为质量标志物。采用同一HPLC法测定10批样品中上述差异性成分的含量。结果网络药理学检索得到槲皮素、木犀草素、绿原酸等黄酮类和有机酸类共10种主要活性成分，与抗COPD密切相关的靶点有91个。10批样品中共指认出9个共有峰，相似度均大于0.90，其中绿原酸、咖啡酸、1，3-O-二咖啡酰基奎尼酸、芹菜素7-O-β-D-吡喃葡萄糖苷为差异性成分；S3、S4、S6、S7、S10聚为一类，S2、S5、S8、S9聚为一类，S1聚为一类。10批鼠曲草药材中绿原酸、咖啡酸、1，3-O-二咖啡酰基奎尼酸、芹菜素7-O-β-D-吡喃葡萄糖苷的含量分别为0.070～7.653、0.010～0.097、0.001～0.036、0.508～6.627mg/g。结论绿原酸、咖啡酸、1，3-O-二咖啡酰基奎尼酸、芹菜素7-O-β-D-吡喃葡萄糖苷可作为鼠曲草的潜在质量标志物，其中绿原酸成分含量以江西吉安产鼠曲草最高，咖啡酸成分含量以江西吉安与福建三明产鼠曲草最高，后两种成分含量以广东潮汕产鼠曲草最高。 |
| ABSTRACT： | OBJECTIVE To screen the quality biomarkers of Gnaphalium affine with anti-chronic obstructive pulmonary disease （COPD） effect and determine their contents. METHODS The effective components and targets of “G. affine” with anti- COPD effect were predicted by using network pharmacology as a search criterion. HPLC fingerprints for 10 batches of G. affine were established by using Similarity Evaluation System of TCM Chromatographic Fingerprint （2012 edition）； common peak identification and similarity evaluation were conducted； cluster analysis （CA）， principal component analysis （PCA）， and orthogonal partial least squares-discriminant analysis （OPLS-DA） were performed to screen differential components as quality maker that affected the quality of G. affine using variable importance projection （VIP）＞1 as the standard. The same HPLC method was adopted to determine the contents of the differential components in 10 batches of samples. RESULTS A total of 10 flavonoids （such as quercetin， luteolin， and chlorogenic acid） and organic acid components， were identified through network pharmacology search， with 91 targets closely related to anti-COPD. A total of 9 common peaks were identified in 10 batches of samples， with similarity greater than 0.90. Among them， the differential components included chlorogenic acid， caffeic acid， 1，3-O- dicaffeoylquinic acid and apigenin 7-O-β-D-glucopyranoside； S3， S4， S6， S7 and S10 were clustered into one category， S2， S5， S8 and S9 clustered into one category， and S1 clustered into one category. The contents of chlorogenic acid， caffeic acid， 1，3-O- dicaffeoylquinic acid， and apigenin 7-O-β-D-glucopyranoside in 10 batches of G. affine ranged 0.070-7.653， 0.010-0.097， 0.001- 0.036， 0.508-6.627 mg/g， respectively. CONCLUSIONS Chlorogenic acid， caffeic acid， 1，3-O-dicaffeoylquinic acid， apigenin 7- O-β-D-glucopyranoside can serve as the potential quality marker for the anti-COPD effect of G. affine， with the highest content of chlorogenic acid in G. affine produced in Ji’an， Jiangxi province， and the highest content of caffeic acid in G. affine produced in Ji’an， Jiangxi province and Sanming， Fujian province. The contents of the last two components are highest in G. affine produced in Chaoshan， Guangdong province. |
| 期刊： | 2024年第35卷第06期 |
| 作者： | 刘婉婷；谢榕；林达淮；叶向丽；严国鸿；李煌 |
| AUTHORS： | LIU Wanting，XIE Rong，LIN Dahuai，YE Xiangli，YAN Guohong，LI Huang |
| 关键字： | 鼠曲草；靶点预测；指纹图谱；慢性阻塞性肺疾病；质量标志物 |
| KEYWORDS： | Gnaphalium affine； target prediction； finger- |
| 阅读数： | 36 次 |
| 本月下载数： | 10 次 |
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