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甘草甜素对大鼠幽门螺杆菌相关性胃炎的改善作用及机制
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| 篇名： | 甘草甜素对大鼠幽门螺杆菌相关性胃炎的改善作用及机制 |
| TITLE： | Improvement effects of glycyrrhizin on Helicobacter pylori-associated gastritis in rats and its mechanism |
| 摘要： | 目的 探讨甘草甜素（GL）对大鼠幽门螺杆菌（HP）相关性胃炎的改善作用及机制。方法以接种1×109cfu/mLHP建立HP相关性胃炎大鼠模型，将造模成功大鼠随机分为模型组、阳性对照组（HP标准四联方案）和GL低、中、高剂量组（5、20、50mg/kg），每组12只；另取12只正常大鼠作为正常对照组。除正常对照组和模型组大鼠灌胃等体积生理盐水外，其他各组大鼠灌胃相应药物，每天1次，连续30d。给药结束后大鼠行13C尿素呼气试验，记录超基准值（DOB）；观察大鼠胃黏膜组织病理变化和细胞形态学变化，并进行病理评分；检测大鼠胃黏膜组织中白细胞介素8（IL-8）、IL-1β、肿瘤坏死因子α（TNF-α）、活性氧（ROS）、丙二醛（MDA）水平，高迁移率族蛋白B1（HMGB1）、核因子κB（NF-κB）mRNA相对表达量，一氧化氮合酶（iNOS）、HMGB1蛋白相对表达量以及NF-κBp65磷酸化水平。结果与正常对照组比较，模型组大鼠DOB值，胃黏膜组织病理评分，IL-8、IL-1β、TNF-α、ROS、MDA水平，HMGB1、NF-κBmRNA相对表达量，iNOS、HMGB1蛋白相对表达量和NF-κBp65磷酸化水平均显著升高（P＜0.05）；大鼠胃黏膜上皮细胞结构不完整且数量减少，细胞碎片及空泡增多，细胞固缩明显。与模型组比较，GL各剂量组和阳性对照组上述指标变化均显著逆转（P＜0.05），且GL高剂量组上述指标变化均较GL低、中剂量组更显著（P＜0.05）；大鼠胃黏膜细胞病理变化均改善。结论GL可能通过抑制HMGB1/NF-κB信号通路的激活，抑制炎症和氧化应激反应，从而减轻HP引起的胃黏膜损伤。 |
| ABSTRACT： | OBJECTIVE To investigate the improvement effects of glycyrrhizin （GL） on Helicobacter pylori （HP）-associated gastritis in rats and its mechanism. METHODS HP-associated gastritis rat model was induced by inoculating with 1×109 cfu/mL HP. The model rats were randomly divided into model group， positive control group （HP standard quadruple group）， GL low-dose， medium-dose and high-dose groups （5， 20， 50 mg/kg）， with 12 rats in each group. Another 12 healthy rats were selected as normal control group. Except the normal control group and model group were given constant volume of normal saline intragastrically， the other groups were given corresponding drugs intragastrically， once a day， for 30 consecutive days. After administration， rats received 13C urea breath test， and delta-over-baseline （DOB） was recorded； the pathological and cellular morphological changes of gastric mucosa in rats were observed， and pathological scoring was performed； the levels of interleukin-8 （IL-8）， IL-1β， tumor necrosis factor-α （TNF-α）， reactive oxygen species （ROS） and malondialdehyde （MDA） were detected in gastric mucosa of rats； mRNA expressions of high mobility group box-1 protein （HMGB1） and nuclear factor-κ-B （NF-κB）， relative expressions of nitric oxide synthases （iNOS） and HMGB1， the phosphorylation level of NF- κBp65 were also detected in rats. RESULTS Compared with normal control group， the DOB value， histopathological score of gastric mucosa， the levels of IL-8， IL-1β， TNF-α， ROS and MDA， relative expressions of HMGB1 and NF- κB mRNA， relative expressions of iNOS and HMGB1 protein and the phosphorylation level of NF-κB p65 were all increased significantly in model group （P＜0.05）； the epithelial cells of gastric mucosa in rats were incomplete in structure and decreased in the number， with an increase in cell fragments and vacuoles， and significant cell pyknosis. Compared with model group， the changes of the above indexes in GL groups and positive control group were significantly reversed （P＜0.05）； the changes in the above indicators in the GL high-dose group were more significant than GL low-dose and medium-dose groups （P＜0.05）； the pathological changes of gastric mucosal cells in rats had all improved. CONCLUSIONS GL may inhibit inflammation and oxidative stress by inhibiting the activation of HMGB1/NF-κB pathway， thus relieving HP-induced gastric mucosal injury. |
| 期刊： | 2024年第35卷第02期 |
| 作者： | 刘誉华；刘莲；汪九重；黄丹；周素芳；肖欢智；安祯祥 |
| AUTHORS： | LIU Yuhua，LIU Lian，WANG Jiuchong，HUANG Dan，ZHOU Sufang，XIAO Huanzhi，AN Zhenxiang |
| 关键字： | 甘草甜素；高迁移率族蛋白B1；核因子κB；幽门螺杆菌相关性胃炎；胃黏膜损伤 |
| KEYWORDS： | glycyrrhizin； high mobility group box 1 protein； nuclear transcription factor-κB； Helicobacter pylori-associated |
| 阅读数： | 146 次 |
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