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光皮木瓜特征图谱及3种黄酮类成分含量测定研究
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| 篇名： | 光皮木瓜特征图谱及3种黄酮类成分含量测定研究 |
| TITLE： | Study on characteristic chromatogram of Chaenomeles sinensis and content determination of 3 flavones |
| 摘要： | 目的 建立光皮木瓜的特征图谱，同时测定芦丁、金丝桃苷、槲皮苷3种黄酮类成分的含量，鉴别光皮木瓜和皱皮木瓜。方法采用高效液相色谱（HPLC）法。以Agilent5TC-C18为色谱柱，以乙腈-0.2%磷酸溶液为流动相进行梯度洗脱，流速为1.0mL/min，柱温为30℃，特征图谱检测波长为330nm，含量测定检测波长为350nm。采用《中药色谱指纹图谱相似度评价系统（2012版）》建立光皮木瓜的特征图谱并评价相似度，使用SPSS23.0软件对15批光皮木瓜（S1～S15）进行聚类分析；测定15批光皮木瓜和7批皱皮木瓜（S16～S22）中3种黄酮类成分的含量，比较二者特征图谱。结果15批光皮木瓜样品的特征图谱相似度为0.783～0.969；共确定11个特征峰；指认了4种成分，分别为绿原酸、芦丁、金丝桃苷、槲皮苷。15批光皮木瓜样品聚为2类，S5～S8为一类，其余为一类。光皮木瓜与皱皮木瓜的特征图谱有明显区别。15批光皮木瓜样品中芦丁、金丝桃苷、槲皮苷含量分别为48.99～294.45、3.49～102.55、31.98～149.49μg/g，皱皮木瓜中芦丁含量低于光皮木瓜，且皱皮木瓜中均未检出金丝桃苷（S20样品除外）和槲皮苷。结论所建立的光皮木瓜的特征图谱和3种黄酮类成分的含量测定方法可用于其质量控制及其与皱皮木瓜的鉴别。 |
| ABSTRACT： | OBJECTIVE To establish the characteristic chromatogram of Chaenomeles sinensis， determine the contents of rutin， hyperin and quercitrin， and to identify C. sinensis and C. speciosa. METHODS HPLC method was performed on Agilent 5 TC-C18 column， with acetonitrile-0.2% formic acid solution as the mobile phase for gradient elution， at the flow rate of 1.0 mL/min. The column temperature was 30 ℃ . The detection wavelength was 330 nm in characteristic chromatogram and 350 nm in content determination. The characteristic chromatogram of C. sinensis was established and similarity was evaluated by the Similarity Evaluation System for Chromatographic Fingerprint of TCM （2012 edition）. Hierarchical cluster analysis of 15 batches of C. sinensis （S1-S15） was performed by using SPSS 23.0 software. The contents of 3 flavones in 15 batches of C. sinensis and 7 batches of C. speciosa （S16-S22） were determined， while their characteristic chromatograms were compared. RESULTS The similarities of the characteristic chromatogram for 15 batches of C. sinensis ranged from 0.783 to 0.969， and 11 characteristic peaks were confirmed. Four constituents were identified as chlorogenic acid， rutin， hyperin and quercitrin. The medicinal materials in 15 batches of C. sinensis could be divided into 2 categories： S5-S8 were one category， and the others belonged to one category. The characteristic chromatogram of C. sinensis was obviously different from C. speciosa. The contents of rutin， hyperin and quercitrin in 15 batches of C. sinensis were 48.99-294.45， 3.49-102.55， 31.98-149.49 μg/g， respectively. The content of rutin in C. speciosa was lower than that in C. sinensis. None of hyperin （except for S20） and quercitrin were detected in C. speciosa. CONCLUSIONS The characteristic chromatogram and the method for content determination of 3 flavones in C. sinensis are established successfully and can be used for the quality control of C. sinensis and its identification from C. speciosa. |
| 期刊： | 2024年第35卷第02期 |
| 作者： | 朱田密；陈树和；严劲松；王新桂；段雨晴；杨孝义 |
| AUTHORS： | ZHU Tianmi，CHEN Shuhe，YAN Jingsong，WANG Xingui，DUAN Yuqing，YANG Xiaoyi |
| 关键字： | 光皮木瓜；黄酮；特征图谱；高效液相色谱法 |
| KEYWORDS： | Chaenomeles sinensis； flavones； characteristic chromatogram； HPLC |
| 阅读数： | 164 次 |
| 本月下载数： | 71 次 |
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