麻黄碱对脂多糖诱导的小胶质细胞功能损伤的修复作用及机制
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篇名: 麻黄碱对脂多糖诱导的小胶质细胞功能损伤的修复作用及机制
TITLE: Repair effect of ephedrine on lipopolysaccharide-induced microglia function injury and its mechanism
摘要: 目的 研究麻黄碱对脂多糖(LPS)诱导的小胶质细胞功能损伤的修复作用及机制。方法以人小胶质细胞HMC3为研究对象,考察不同质量浓度麻黄碱(75、150、300、600μg/mL)对HMC3细胞活力和凋亡的影响。然后将HMC3细胞分为对照组(不受药物干预)、LPS组(1μg/mL)、麻黄碱组(1μg/mLLPS+300μg/mL麻黄碱)、BAY11-7082组[1μg/mLLPS+5μmol/L核因子κB(NF-κB)信号通路抑制剂BAY11-7082]、抑制剂组(1μg/mLLPS+300μg/mL麻黄碱+5μmol/LBAY11-7082)和激活剂组(1μg/mLLPS+300μg/mL麻黄碱+1μmol/LNF-κB信号通路激活剂Prostratin),加入相应药物作用24h后,检测细胞迁移能力和细胞中可溶性白细胞介素6(sIL-6)、白细胞介素10(IL-10)、超氧化物歧化酶(SOD)、丙二醛(MDA)水平以及NF-κB信号通路相关蛋白表达水平。结果300μg/mL的麻黄碱可使HMC3细胞活力显著升高、凋亡率显著降低(P<0.05)。与对照组相比,LPS组迁移细胞数显著增多,细胞中sIL-6、MDA水平和NF-κB蛋白磷酸化水平均显著升高,IL-10、SOD水平均显著降低(P<0.05)。与LPS组相比,麻黄碱组和BAY11-7082组上述指标水平均显著逆转(P<0.05)。与麻黄碱组相比,抑制剂组迁移细胞数显著减少,细胞中sIL-6、MDA水平和NF-κB蛋白磷酸化水平均显著降低,IL-10、SOD水平均显著升高(P<0.05);而激活剂组上述指标水平均显著逆转(P<0.05)。结论麻黄碱可通过抑制LPS诱导的HMC3细胞凋亡、迁移、炎症和氧化应激,修复细胞功能损伤,其作用机制可能与抑制NF-κB信号通路活性有关。
ABSTRACT: OBJECTIVE To study the repair effect of ephedrine on lipopolysaccharide (LPS)-induced microglia function injury and its mechanism. METHODS Human microglia cells (HMC3) were used as research objects to investigate the effects of different concentrations of ephedrine (75, 150, 300, 600 μg/mL) on the viability and apoptosis of HMC3 cells. HMC3 cells were divided into control group (without drug intervention), LPS group (1 μg/mL), ephedrine group (1 μg/mL LPS+300 μg/mL ephedrine), BAY11-7082 group [1 μg/mL LPS+5 μmol/L nuclear factor-κB (NF-κB) pathway inhibitor BAY11-7082], inhibitor group (1 μg/mL LPS+300 μg/mL ephedrine+5 μmol/L BAY11-7082) and activator group (1 μg/mL LPS+300 μg/mL ephedrine+1 μmol/L NF-κB pathway activator Prostratin). After 24 hours of drug treatment, cell migration, the levels of soluble interleukin-6(sIL-6), interleukin-10(IL-10), superoxide dismutase(SOD)and malondialdehyde(MDA), and the expressions of NF-κB pathway-related proteins were all detected. RESULTS The viability of HMC3 cells could be increased significantly by 300 μg/mL ephedrine, while the apoptotic rate was decreased significantly (P<0.05). Compared with the control group, the number of migrating cells was increased significantly in the LPS group; the levels of sIL-6 and MDA, the phosphorylation of NF-κB protein were increased significantly, while the levels of IL-10 and SOD were decreased significantly (P<0.05). Compared with the LPS group, the above indexes were reversed significantly in the ephedrine group and BAY11-7082 group (P<0.05). Compared with the ephedrine group, the number of migrating cells was decreased significantly in the inhibitor group; the levels of sIL-6 and MDA, the phosphorylation of NF-κB protein were decreased significantly, while the levels of IL-10 and SOD were increased significantly (P<0.05). The above indexes were reversed significantly in the activator group (P<0.05)can repair cell injury by inhibiting LPS induced apoptosis, migration, inflammation and oxidant stress of HMC3 cells, the mechanism of which may be associated with inhibiting the activity of the NF-κB signaling pathway.
期刊: 2024年第35卷第01期
作者: 尹涛;姜丽真;张盟盟;王睿健;张文超
AUTHORS: YIN Tao,JIANG Lizhen,ZHANG Mengmeng,WANG Ruijian,ZHANG Wenchao
关键字: 小胶质细胞;麻黄碱;脂多糖;核因子κB信号通路;细胞功能损伤
KEYWORDS: microglia cells; ephedrine; lipopolysaccharide; nuclear factor-κB signaling pathway; cell function injury
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