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水冬瓜根及茎的质量控制方法研究
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| 篇名： | 水冬瓜根及茎的质量控制方法研究 |
| TITLE： | Study on quality control method of the roots and rhizoma of Toricellia angulata |
| 摘要： | 目的 建立水冬瓜根及茎药材的质量控制方法。方法观察水冬瓜根及茎药材的性状、显微特征；按2020年版《中国药典》（四部）通则方法对水冬瓜根及茎药材中的水分、总灰分、酸不溶性灰分和醇溶性浸出物进行测定；采用《中药色谱指纹图谱相似度评价系统（2012版）》建立11批水冬瓜根及茎药材的高效液相色谱（HPLC）指纹图谱，并进行共有峰的指认及相似度评价；采用HPLC法测定水冬瓜根及茎中松柏苷、紫丁香苷、绿原酸、（+）-syringaresinol-O-β-D-glucopyranoside、丁香脂素的含量。结果水冬瓜根及茎药材性状与显微鉴别特征明显。水分、总灰分、酸不溶性灰分和醇溶性浸出物的含量平均值分别为7.54%、2.18%、0.15%、7.81%。11批水冬瓜根及茎药材的指纹图谱有16个共有峰，相似度为0.856～0.960，共指认出松柏苷、紫丁香苷、绿原酸、（+）-syringaresinol-O-β-D-glucopyranoside、丁香脂素5个共有成分。上述5个成分的含量分别为0.0472～0.4016、0.8368～8.6979、1.2453～10.9500、0.1390～0.4378、0.0164～0.6353mg/g。结论所建方法稳定、准确性好，可用于水冬瓜根及茎药材的质量控制。初步拟定水冬瓜根及茎药材中水分不得过11.0%，总灰分不得过4.0%，醇溶性浸出物不得少于5.0%，松柏苷、紫丁香苷、绿原酸、（+）-syringaresinol-O-β-D-glucopyranoside、丁香脂素含量分别不得少于0.04、0.83、1.24、0.13、0.01mg/g。 |
| ABSTRACT： | OBJECTIVE To establish the quality control method for the roots and rhizoma of Toricellia angulata. METHODS The properties of the roots and rhizoma of T. angulata were observed and microscopic identification was conducted. The moisture， total ash， acid-insoluble ash and ethanol-soluble extract were examined according to the method stated in the 2020 edition of Chinese Pharmacopoeia （part Ⅳ）. HPLC fingerprints of 11 batches of the roots and rhizoma of T. angulata were established， common peaks were identified and the similarity was evaluated by using the Similarity Evaluation System of Chromatographic Fingerprint of TCM （2012 edition）. The contents of coniferin， syringin， chlorogenic acid， （+）-syringaresinol-O-β-D-glucopyranoside and syringaresinol were determined by HPLC. RESULTS The properties and microscopic identification of the roots and rhizoma of T. angulata were obvious. The average contents of moisture， total ash， acid-insoluble ash and ethanol-soluble extract were 7.54%， 2.18%， 0.15% and 7.81%， respectively. There were 16 common peaks marked in the HPLC fingerprints of 11 batches of the roots and rhizoma of T. angulata， with similarities of 0.856-0.960； five of them were identified， such as coniferin， syringin， chlorogenic acid， （+）-syringaresinol-O-β-D-glucopyranoside and syringaresinol. The contents of the above five components were 0.047 2-0.401 6， 0.836 8-8.697 9， 1.245 3-10.950 0， 0.139 0-0.437 8 and 0.016 4-0.635 3 mg/g， respectively. CONCLUSIONS The established method is stable and accurate， which can be used for the quality control of the roots and rhizoma of T. angulata. It is preliminarily proposed that the moisture in the roots and rhizoma of T. angulata is not more than 11.0%， the total ash is not more than 4.0%， the ethanol-soluble extract is not less than 5.0%， the contents of coniferin， syringin， chlorogenic acid， （+）-syringaresinol-O-β-D- glucopyranoside and syringaresinol are not less than 0.04，0.83， 1.24， 0.13， 0.01 mg/g， respectively. |
| 期刊： | 2024年第35卷第01期 |
| 作者： | 黎雪；聂雨杉；马雪；陆苑；杨畅；李勇军；王永林 |
| AUTHORS： | LI Xue，NIE Yushan，MA Xue，LU Yuan，YANG Chang，LI Yongjun，WANG Yonglin |
| 关键字： | 水冬瓜根；水冬瓜茎；性状鉴别；显微鉴别；指纹图谱；含量测定；质量控制 |
| KEYWORDS： | roots of Toricellia angulata； rhizoma of |
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